Immunocytochemical assay of estrogen receptors in endometrial carcinoma with monoclonal antibodies. Comparison with biochemical assay
Specimens of endometrial adenocarcinoma, surgically obtained from 18 women, were analyzed for distribution of estrogen receptors by an immunocytochemical assay, employing monoclonal anti‐estrophilin antibodies and the peroxidase‐antiperoxidase technique. Results were compared with biochemical recept...
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| Veröffentlicht in: | Cancer 1986-03, Vol.57 (5), p.1000-1004 |
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| container_title | Cancer |
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| creator | Pertschuk, Louis P. Beddoe, Anne Marie Gorelic, Lester S. Shain, Sydney A. |
| description | Specimens of endometrial adenocarcinoma, surgically obtained from 18 women, were analyzed for distribution of estrogen receptors by an immunocytochemical assay, employing monoclonal anti‐estrophilin antibodies and the peroxidase‐antiperoxidase technique. Results were compared with biochemical receptor analyses, and were in concordance in 83% of them. Marked tumor cell and tissue receptor heterogeneity were apparent with the immunocytochemical method, and a variety of patterns of nuclear staining in positive tissue samples were revealed. These results indicate that the immunohistologic method will provide a number of entirely new variables that may eventually be correlated with both clinical and pathologic features of this malignancy, and may prove to be of value in the prediction of clinical endocrine response. |
| doi_str_mv | 10.1002/1097-0142(19860301)57:5<1000::AID-CNCR2820570521>3.0.CO;2-9 |
| format | Article |
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These results indicate that the immunohistologic method will provide a number of entirely new variables that may eventually be correlated with both clinical and pathologic features of this malignancy, and may prove to be of value in the prediction of clinical endocrine response.</description><identifier>ISSN: 0008-543X</identifier><identifier>EISSN: 1097-0142</identifier><identifier>DOI: 10.1002/1097-0142(19860301)57:5<1000::AID-CNCR2820570521>3.0.CO;2-9</identifier><identifier>PMID: 3510707</identifier><identifier>CODEN: CANCAR</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adenocarcinoma - metabolism ; Adenocarcinoma - surgery ; Aged ; Antibodies, Monoclonal ; Biological and medical sciences ; Cell Nucleus - metabolism ; Cytosol - metabolism ; False Positive Reactions ; Female ; Female genital diseases ; Frozen Sections ; Gynecology. Andrology. Obstetrics ; Histocytochemistry ; Humans ; Hysterectomy ; Immunoenzyme Techniques ; Medical sciences ; Middle Aged ; Receptors, Estrogen - analysis ; Tumors ; Uterine Neoplasms - metabolism ; Uterine Neoplasms - surgery</subject><ispartof>Cancer, 1986-03, Vol.57 (5), p.1000-1004</ispartof><rights>Copyright © 1986 American Cancer Society</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4141-fde62bfe35f4f0c481219e633441c943c00df72f7f27b8d6869a67c004baa97e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8604042$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3510707$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pertschuk, Louis P.</creatorcontrib><creatorcontrib>Beddoe, Anne Marie</creatorcontrib><creatorcontrib>Gorelic, Lester S.</creatorcontrib><creatorcontrib>Shain, Sydney A.</creatorcontrib><title>Immunocytochemical assay of estrogen receptors in endometrial carcinoma with monoclonal antibodies. Comparison with biochemical assay</title><title>Cancer</title><addtitle>Cancer</addtitle><description>Specimens of endometrial adenocarcinoma, surgically obtained from 18 women, were analyzed for distribution of estrogen receptors by an immunocytochemical assay, employing monoclonal anti‐estrophilin antibodies and the peroxidase‐antiperoxidase technique. Results were compared with biochemical receptor analyses, and were in concordance in 83% of them. Marked tumor cell and tissue receptor heterogeneity were apparent with the immunocytochemical method, and a variety of patterns of nuclear staining in positive tissue samples were revealed. These results indicate that the immunohistologic method will provide a number of entirely new variables that may eventually be correlated with both clinical and pathologic features of this malignancy, and may prove to be of value in the prediction of clinical endocrine response.</description><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - surgery</subject><subject>Aged</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytosol - metabolism</subject><subject>False Positive Reactions</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Frozen Sections</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Hysterectomy</subject><subject>Immunoenzyme Techniques</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Receptors, Estrogen - analysis</subject><subject>Tumors</subject><subject>Uterine Neoplasms - metabolism</subject><subject>Uterine Neoplasms - surgery</subject><issn>0008-543X</issn><issn>1097-0142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkV2L1DAUhoso6-zqTxB6IaIXHU--mmYUYalfA4sDoiB4EdI0cSNNMyYdlvkB_u9NmXHAvRC8Csn7nPecnLcoWgRLBIBfIhC8AkTxcySaGgigF4yv2Osswmp1uX5btZ_az7jBwDgwjN6QJSzbzStciXvF4lR9v1jkgqZilHx7WJyn9DNfOWbkrDgjDAEHvih-r73fjUHvp6CvjXdaDaVKSe3LYEuTphh-mLGMRpvtFGIq3ViasQ_eTNFlVKuo3Ri8Km_cdF36kK2GMM4m4-S60DuTlmUb_FZFl8J4wDp3p9mj4oFVQzKPj-dF8fX9uy_tx-pq82HdXl5VmiKKKtubGnfWEGapBU0bhJEwNSGUIi0o0QC95dhyi3nX9HVTC1Xz_Eo7pQQ35KJ4dvDdxvBrl78nvUvaDIMaTdglyeu6EQKaDH4_gDqGlKKxchudV3EvEcg5JDmvWc5rln9CkoxLNosgZQ5J_h2SJBJku5FYiuz-5DjGrvOmP3kfU8n606OuUt6RjWrULp2w3I4CxRmzB-zGDWb_fxP-c8A7CrkF0x--wQ</recordid><startdate>19860301</startdate><enddate>19860301</enddate><creator>Pertschuk, Louis P.</creator><creator>Beddoe, Anne Marie</creator><creator>Gorelic, Lester S.</creator><creator>Shain, Sydney A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860301</creationdate><title>Immunocytochemical assay of estrogen receptors in endometrial carcinoma with monoclonal antibodies. 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Obstetrics</topic><topic>Histocytochemistry</topic><topic>Humans</topic><topic>Hysterectomy</topic><topic>Immunoenzyme Techniques</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Receptors, Estrogen - analysis</topic><topic>Tumors</topic><topic>Uterine Neoplasms - metabolism</topic><topic>Uterine Neoplasms - surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pertschuk, Louis P.</creatorcontrib><creatorcontrib>Beddoe, Anne Marie</creatorcontrib><creatorcontrib>Gorelic, Lester S.</creatorcontrib><creatorcontrib>Shain, Sydney A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pertschuk, Louis P.</au><au>Beddoe, Anne Marie</au><au>Gorelic, Lester S.</au><au>Shain, Sydney A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunocytochemical assay of estrogen receptors in endometrial carcinoma with monoclonal antibodies. Comparison with biochemical assay</atitle><jtitle>Cancer</jtitle><addtitle>Cancer</addtitle><date>1986-03-01</date><risdate>1986</risdate><volume>57</volume><issue>5</issue><spage>1000</spage><epage>1004</epage><pages>1000-1004</pages><issn>0008-543X</issn><eissn>1097-0142</eissn><coden>CANCAR</coden><abstract>Specimens of endometrial adenocarcinoma, surgically obtained from 18 women, were analyzed for distribution of estrogen receptors by an immunocytochemical assay, employing monoclonal anti‐estrophilin antibodies and the peroxidase‐antiperoxidase technique. Results were compared with biochemical receptor analyses, and were in concordance in 83% of them. Marked tumor cell and tissue receptor heterogeneity were apparent with the immunocytochemical method, and a variety of patterns of nuclear staining in positive tissue samples were revealed. These results indicate that the immunohistologic method will provide a number of entirely new variables that may eventually be correlated with both clinical and pathologic features of this malignancy, and may prove to be of value in the prediction of clinical endocrine response.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>3510707</pmid><doi>10.1002/1097-0142(19860301)57:5<1000::AID-CNCR2820570521>3.0.CO;2-9</doi><tpages>5</tpages></addata></record> |
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| ispartof | Cancer, 1986-03, Vol.57 (5), p.1000-1004 |
| issn | 0008-543X 1097-0142 |
| language | eng |
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| subjects | Adenocarcinoma - metabolism Adenocarcinoma - surgery Aged Antibodies, Monoclonal Biological and medical sciences Cell Nucleus - metabolism Cytosol - metabolism False Positive Reactions Female Female genital diseases Frozen Sections Gynecology. Andrology. Obstetrics Histocytochemistry Humans Hysterectomy Immunoenzyme Techniques Medical sciences Middle Aged Receptors, Estrogen - analysis Tumors Uterine Neoplasms - metabolism Uterine Neoplasms - surgery |
| title | Immunocytochemical assay of estrogen receptors in endometrial carcinoma with monoclonal antibodies. Comparison with biochemical assay |
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