Dynamic aspects of affinity labelling as revealed by alkylation and phosphorylation of pancreatic ribonuclease with reactive deoxyribodinucleotide derivatives
Affinity labelling of pancreatic RNase with 4-( N-2-chloroethyl- N-methylamino)benzylamide and (N → P) N-methylimidazolide of d(pTpA) results in the formation of monomodified enzyme derivatives retaining partially enzymatic activity. These data together with some cases described in the literature ar...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 1986, Vol.194 (1), p.64-68 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 68 |
|---|---|
| container_issue | 1 |
| container_start_page | 64 |
| container_title | FEBS letters |
| container_volume | 194 |
| creator | Knorre, D.G. Buneva, V.N. Baram, G.I. Godovikova, T.S. Zarytova, V.F. |
| description | Affinity labelling of pancreatic RNase with 4-(
N-2-chloroethyl-
N-methylamino)benzylamide and (N → P)
N-methylimidazolide of
d(pTpA) results in the formation of monomodified enzyme derivatives retaining partially enzymatic activity. These data together with some cases described in the literature are considered as suggesting the dynamic nature of the enzyme-reagent complex represented by a set of states differing in the probability of intra-complex reaction. In particular, modification may proceed in a low probability state with an especially favorable mutual orientation of reagent and some protein residue remote from the active site of the enzyme resulting in the removal of the covalently attached reagent moiety from the active center. |
| doi_str_mv | 10.1016/0014-5793(86)80052-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76688169</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0014579386800527</els_id><sourcerecordid>76688169</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5557-6b1f0d5a149deb68237d0ad98aec616cfd0308ba22774b7a6c1a0fa9e470502c3</originalsourceid><addsrcrecordid>eNqNks2OFCEUhYnRjO3oG2jCwhhdlEL9ALWZRMdpNZnEja7JLbjloNVFCdU91sv4rEJ320tHEkK45-PwcyDkKWevOePiDWO8LhrZVi-VeKUYa8pC3iMrrmRVVLVQ98nqhDwkj2L8ztJc8faMnFVtzVTLVuT3-2WEjTMU4oRmjtT3FPrejW5e6AAdDoMbvyWVBtwhDGhpt1AYfiwDzM6PFEZLpxsfUw9_a8ljgtEETFNDg-v8uDUDQkR66-abZAVmdjukFv2vJevW7Qk_O5urwe0gA_ExedDDEPHJcTwnX9dXXy4_FtefP3y6fHtdmKZpZCE63jPbAK9bi51QZSUtA9sqQCO4ML1lFVMdlKWUdSdBGA6shxZryRpWmuqcvDj4TsH_3GKc9cZFky4PI_pt1FIIpbho7wR5w6pKcJXA-gCa4GMM2OspuA2ERXOmc346h6NzOFoJvc9Py7Ts2dF_223QnhYdA0v686MO0cDQh_TOLp4wxUrFZXMXJluRWt5tfcBu3YDLf51Qr6_elVnIdSX21Wx0cTDCFNLOYdDROBwNWhfSt9LWu3_f-w87Hdsu</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15033618</pqid></control><display><type>article</type><title>Dynamic aspects of affinity labelling as revealed by alkylation and phosphorylation of pancreatic ribonuclease with reactive deoxyribodinucleotide derivatives</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Knorre, D.G. ; Buneva, V.N. ; Baram, G.I. ; Godovikova, T.S. ; Zarytova, V.F.</creator><creatorcontrib>Knorre, D.G. ; Buneva, V.N. ; Baram, G.I. ; Godovikova, T.S. ; Zarytova, V.F.</creatorcontrib><description>Affinity labelling of pancreatic RNase with 4-(
N-2-chloroethyl-
N-methylamino)benzylamide and (N → P)
N-methylimidazolide of
d(pTpA) results in the formation of monomodified enzyme derivatives retaining partially enzymatic activity. These data together with some cases described in the literature are considered as suggesting the dynamic nature of the enzyme-reagent complex represented by a set of states differing in the probability of intra-complex reaction. In particular, modification may proceed in a low probability state with an especially favorable mutual orientation of reagent and some protein residue remote from the active site of the enzyme resulting in the removal of the covalently attached reagent moiety from the active center.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(86)80052-7</identifier><identifier>PMID: 3940890</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Affinity labeling Biopolymer dynamics Reactive dinucleotide derivative Pancreatic RNase ; Affinity Labels ; Alkylation ; Analytical, structural and metabolic biochemistry ; Applied sciences ; Binding Sites ; Biological and medical sciences ; Chromatography - methods ; Deoxyribonucleotides - pharmacology ; Enzymes and enzyme inhibitors ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Hydrolases ; Kinetics ; Microchemistry ; Other techniques and industries ; Pancreas - enzymology ; Phosphorylation ; Ribonucleases - antagonists & inhibitors ; Ribonucleases - metabolism</subject><ispartof>FEBS letters, 1986, Vol.194 (1), p.64-68</ispartof><rights>1986</rights><rights>FEBS Letters 194 (1986) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5557-6b1f0d5a149deb68237d0ad98aec616cfd0308ba22774b7a6c1a0fa9e470502c3</citedby><cites>FETCH-LOGICAL-c5557-6b1f0d5a149deb68237d0ad98aec616cfd0308ba22774b7a6c1a0fa9e470502c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579386800527$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7966667$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8028175$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3940890$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Knorre, D.G.</creatorcontrib><creatorcontrib>Buneva, V.N.</creatorcontrib><creatorcontrib>Baram, G.I.</creatorcontrib><creatorcontrib>Godovikova, T.S.</creatorcontrib><creatorcontrib>Zarytova, V.F.</creatorcontrib><title>Dynamic aspects of affinity labelling as revealed by alkylation and phosphorylation of pancreatic ribonuclease with reactive deoxyribodinucleotide derivatives</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Affinity labelling of pancreatic RNase with 4-(
N-2-chloroethyl-
N-methylamino)benzylamide and (N → P)
N-methylimidazolide of
d(pTpA) results in the formation of monomodified enzyme derivatives retaining partially enzymatic activity. These data together with some cases described in the literature are considered as suggesting the dynamic nature of the enzyme-reagent complex represented by a set of states differing in the probability of intra-complex reaction. In particular, modification may proceed in a low probability state with an especially favorable mutual orientation of reagent and some protein residue remote from the active site of the enzyme resulting in the removal of the covalently attached reagent moiety from the active center.</description><subject>Affinity labeling Biopolymer dynamics Reactive dinucleotide derivative Pancreatic RNase</subject><subject>Affinity Labels</subject><subject>Alkylation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Applied sciences</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Chromatography - methods</subject><subject>Deoxyribonucleotides - pharmacology</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolases</subject><subject>Kinetics</subject><subject>Microchemistry</subject><subject>Other techniques and industries</subject><subject>Pancreas - enzymology</subject><subject>Phosphorylation</subject><subject>Ribonucleases - antagonists & inhibitors</subject><subject>Ribonucleases - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks2OFCEUhYnRjO3oG2jCwhhdlEL9ALWZRMdpNZnEja7JLbjloNVFCdU91sv4rEJ320tHEkK45-PwcyDkKWevOePiDWO8LhrZVi-VeKUYa8pC3iMrrmRVVLVQ98nqhDwkj2L8ztJc8faMnFVtzVTLVuT3-2WEjTMU4oRmjtT3FPrejW5e6AAdDoMbvyWVBtwhDGhpt1AYfiwDzM6PFEZLpxsfUw9_a8ljgtEETFNDg-v8uDUDQkR66-abZAVmdjukFv2vJevW7Qk_O5urwe0gA_ExedDDEPHJcTwnX9dXXy4_FtefP3y6fHtdmKZpZCE63jPbAK9bi51QZSUtA9sqQCO4ML1lFVMdlKWUdSdBGA6shxZryRpWmuqcvDj4TsH_3GKc9cZFky4PI_pt1FIIpbho7wR5w6pKcJXA-gCa4GMM2OspuA2ERXOmc346h6NzOFoJvc9Py7Ts2dF_223QnhYdA0v686MO0cDQh_TOLp4wxUrFZXMXJluRWt5tfcBu3YDLf51Qr6_elVnIdSX21Wx0cTDCFNLOYdDROBwNWhfSt9LWu3_f-w87Hdsu</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Knorre, D.G.</creator><creator>Buneva, V.N.</creator><creator>Baram, G.I.</creator><creator>Godovikova, T.S.</creator><creator>Zarytova, V.F.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>1986</creationdate><title>Dynamic aspects of affinity labelling as revealed by alkylation and phosphorylation of pancreatic ribonuclease with reactive deoxyribodinucleotide derivatives</title><author>Knorre, D.G. ; Buneva, V.N. ; Baram, G.I. ; Godovikova, T.S. ; Zarytova, V.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5557-6b1f0d5a149deb68237d0ad98aec616cfd0308ba22774b7a6c1a0fa9e470502c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Affinity labeling Biopolymer dynamics Reactive dinucleotide derivative Pancreatic RNase</topic><topic>Affinity Labels</topic><topic>Alkylation</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Applied sciences</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Chromatography - methods</topic><topic>Deoxyribonucleotides - pharmacology</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolases</topic><topic>Kinetics</topic><topic>Microchemistry</topic><topic>Other techniques and industries</topic><topic>Pancreas - enzymology</topic><topic>Phosphorylation</topic><topic>Ribonucleases - antagonists & inhibitors</topic><topic>Ribonucleases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Knorre, D.G.</creatorcontrib><creatorcontrib>Buneva, V.N.</creatorcontrib><creatorcontrib>Baram, G.I.</creatorcontrib><creatorcontrib>Godovikova, T.S.</creatorcontrib><creatorcontrib>Zarytova, V.F.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Knorre, D.G.</au><au>Buneva, V.N.</au><au>Baram, G.I.</au><au>Godovikova, T.S.</au><au>Zarytova, V.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic aspects of affinity labelling as revealed by alkylation and phosphorylation of pancreatic ribonuclease with reactive deoxyribodinucleotide derivatives</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1986</date><risdate>1986</risdate><volume>194</volume><issue>1</issue><spage>64</spage><epage>68</epage><pages>64-68</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Affinity labelling of pancreatic RNase with 4-(
N-2-chloroethyl-
N-methylamino)benzylamide and (N → P)
N-methylimidazolide of
d(pTpA) results in the formation of monomodified enzyme derivatives retaining partially enzymatic activity. These data together with some cases described in the literature are considered as suggesting the dynamic nature of the enzyme-reagent complex represented by a set of states differing in the probability of intra-complex reaction. In particular, modification may proceed in a low probability state with an especially favorable mutual orientation of reagent and some protein residue remote from the active site of the enzyme resulting in the removal of the covalently attached reagent moiety from the active center.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3940890</pmid><doi>10.1016/0014-5793(86)80052-7</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1986, Vol.194 (1), p.64-68 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76688169 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Affinity labeling Biopolymer dynamics Reactive dinucleotide derivative Pancreatic RNase Affinity Labels Alkylation Analytical, structural and metabolic biochemistry Applied sciences Binding Sites Biological and medical sciences Chromatography - methods Deoxyribonucleotides - pharmacology Enzymes and enzyme inhibitors Exact sciences and technology Fundamental and applied biological sciences. Psychology Hydrolases Kinetics Microchemistry Other techniques and industries Pancreas - enzymology Phosphorylation Ribonucleases - antagonists & inhibitors Ribonucleases - metabolism |
| title | Dynamic aspects of affinity labelling as revealed by alkylation and phosphorylation of pancreatic ribonuclease with reactive deoxyribodinucleotide derivatives |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T03%3A44%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dynamic%20aspects%20of%20affinity%20labelling%20as%20revealed%20by%20alkylation%20and%20phosphorylation%20of%20pancreatic%20ribonuclease%20with%20reactive%20deoxyribodinucleotide%20derivatives&rft.jtitle=FEBS%20letters&rft.au=Knorre,%20D.G.&rft.date=1986&rft.volume=194&rft.issue=1&rft.spage=64&rft.epage=68&rft.pages=64-68&rft.issn=0014-5793&rft.eissn=1873-3468&rft.coden=FEBLAL&rft_id=info:doi/10.1016/0014-5793(86)80052-7&rft_dat=%3Cproquest_cross%3E76688169%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15033618&rft_id=info:pmid/3940890&rft_els_id=0014579386800527&rfr_iscdi=true |