Rapid screening and microbiologic processing of pediatric urine specimens
Urinary nitrite and leukocyte esterase dipstick tests were evaluated as rapid screening procedures to select probable culture-positive urines for direct identification (AutoMicrobic System urine cards) and modified Kirby-Bauer susceptibility testing. Approximately 73% of significant culture-positive...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 1986, Vol.4 (1), p.11-17 |
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| creator | Cannon, Harold J. Goetz, Edward S. Hamoudi, Ayser C. Marcon, Mario J. |
| description | Urinary nitrite and leukocyte esterase dipstick tests were evaluated as rapid screening procedures to select probable culture-positive urines for direct identification (AutoMicrobic System urine cards) and modified Kirby-Bauer susceptibility testing. Approximately 73% of significant culture-positive (〉 10
5 organisms per milliliter, pure culture) urine specimens could be selected by nitrite testing alone with very high specificity (∼99%). The leukocyte esterase test detected 85% of culture-positive urines when used alone and ∼91% when used in combination with nitrite testing (if either test was positive it was considered a positive screening); however, the esterase test was significantly less specific for bacteriuria than the nitrite test. Based on these results, the nitrite test was selected for use as the screening test. Rapid, direct identification and susceptibility tests on screen-positive urines showed 97% correlation with standard testing methods. Significant positive urines processed in this manner could be reported with quantitation, identification, and susceptibility results within 24 hr. |
| doi_str_mv | 10.1016/0732-8893(86)90051-9 |
| format | Article |
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5 organisms per milliliter, pure culture) urine specimens could be selected by nitrite testing alone with very high specificity (∼99%). The leukocyte esterase test detected 85% of culture-positive urines when used alone and ∼91% when used in combination with nitrite testing (if either test was positive it was considered a positive screening); however, the esterase test was significantly less specific for bacteriuria than the nitrite test. Based on these results, the nitrite test was selected for use as the screening test. Rapid, direct identification and susceptibility tests on screen-positive urines showed 97% correlation with standard testing methods. Significant positive urines processed in this manner could be reported with quantitation, identification, and susceptibility results within 24 hr.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/0732-8893(86)90051-9</identifier><identifier>PMID: 3510805</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Anti-Bacterial Agents - pharmacology ; Bacteria - drug effects ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Bacteriuria - diagnosis ; Bacteriuria - microbiology ; Biological and medical sciences ; Child ; Escherichia coli - isolation & purification ; Esterases - analysis ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; In Vitro Techniques ; Leukocytes - enzymology ; Microbial Sensitivity Tests ; Microbiology ; Nitrites - urine ; Reagent Kits, Diagnostic ; Staphylococcus - isolation & purification ; Streptococcus - isolation & purification</subject><ispartof>Diagnostic microbiology and infectious disease, 1986, Vol.4 (1), p.11-17</ispartof><rights>1986</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-e5b3127d8314345af658ec6297ee2d87fae103f770c8ffbd8521ad691af868053</citedby><cites>FETCH-LOGICAL-c386t-e5b3127d8314345af658ec6297ee2d87fae103f770c8ffbd8521ad691af868053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0732889386900519$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,4009,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8814186$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3510805$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cannon, Harold J.</creatorcontrib><creatorcontrib>Goetz, Edward S.</creatorcontrib><creatorcontrib>Hamoudi, Ayser C.</creatorcontrib><creatorcontrib>Marcon, Mario J.</creatorcontrib><title>Rapid screening and microbiologic processing of pediatric urine specimens</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Urinary nitrite and leukocyte esterase dipstick tests were evaluated as rapid screening procedures to select probable culture-positive urines for direct identification (AutoMicrobic System urine cards) and modified Kirby-Bauer susceptibility testing. Approximately 73% of significant culture-positive (〉 10
5 organisms per milliliter, pure culture) urine specimens could be selected by nitrite testing alone with very high specificity (∼99%). The leukocyte esterase test detected 85% of culture-positive urines when used alone and ∼91% when used in combination with nitrite testing (if either test was positive it was considered a positive screening); however, the esterase test was significantly less specific for bacteriuria than the nitrite test. Based on these results, the nitrite test was selected for use as the screening test. Rapid, direct identification and susceptibility tests on screen-positive urines showed 97% correlation with standard testing methods. Significant positive urines processed in this manner could be reported with quantitation, identification, and susceptibility results within 24 hr.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacteria - drug effects</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Bacteriuria - diagnosis</subject><subject>Bacteriuria - microbiology</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Escherichia coli - isolation & purification</subject><subject>Esterases - analysis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Leukocytes - enzymology</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Nitrites - urine</subject><subject>Reagent Kits, Diagnostic</subject><subject>Staphylococcus - isolation & purification</subject><subject>Streptococcus - isolation & purification</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMotVbfQGEWIroYTSYzuWwEKV4KBUF0HTLJSYnMzaQVfHtTO3TpKpD_O4f_fAidE3xLMGF3mNMiF0LSa8FuJMYVyeUBmhLBZY4xx4doukeO0UmMnxiTQpZ4gia0IljgaooWb3rwNosmAHS-W2W6s1nrTehr3zf9yptsCL2BGLdh77IBrNfrkP43wXeQxQGMb6GLp-jI6SbC2fjO0MfT4_v8JV--Pi_mD8vcUMHWOVQ1JQW3gpKSlpV2rBJgWCE5QGEFdxoIpo5zbIRztRVVQbRlkmgnWKpMZ-hqtzf1-tpAXKvWRwNNozvoN1FxxpIBWSSw3IHpmBgDODUE3-rwowhWW4Nqq0dt9SjB1J9BJdPYxbh_U7dg90OjspRfjrmORjcu6M74uMeEICURLGH3OwySi28PQUXjoTNJXwCzVrb3__f4BVeLjKY</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Cannon, Harold J.</creator><creator>Goetz, Edward S.</creator><creator>Hamoudi, Ayser C.</creator><creator>Marcon, Mario J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1986</creationdate><title>Rapid screening and microbiologic processing of pediatric urine specimens</title><author>Cannon, Harold J. ; Goetz, Edward S. ; Hamoudi, Ayser C. ; Marcon, Mario J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-e5b3127d8314345af658ec6297ee2d87fae103f770c8ffbd8521ad691af868053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacteria - drug effects</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Bacteriuria - diagnosis</topic><topic>Bacteriuria - microbiology</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Escherichia coli - isolation & purification</topic><topic>Esterases - analysis</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Leukocytes - enzymology</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Nitrites - urine</topic><topic>Reagent Kits, Diagnostic</topic><topic>Staphylococcus - isolation & purification</topic><topic>Streptococcus - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cannon, Harold J.</creatorcontrib><creatorcontrib>Goetz, Edward S.</creatorcontrib><creatorcontrib>Hamoudi, Ayser C.</creatorcontrib><creatorcontrib>Marcon, Mario J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cannon, Harold J.</au><au>Goetz, Edward S.</au><au>Hamoudi, Ayser C.</au><au>Marcon, Mario J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid screening and microbiologic processing of pediatric urine specimens</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>1986</date><risdate>1986</risdate><volume>4</volume><issue>1</issue><spage>11</spage><epage>17</epage><pages>11-17</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>Urinary nitrite and leukocyte esterase dipstick tests were evaluated as rapid screening procedures to select probable culture-positive urines for direct identification (AutoMicrobic System urine cards) and modified Kirby-Bauer susceptibility testing. Approximately 73% of significant culture-positive (〉 10
5 organisms per milliliter, pure culture) urine specimens could be selected by nitrite testing alone with very high specificity (∼99%). The leukocyte esterase test detected 85% of culture-positive urines when used alone and ∼91% when used in combination with nitrite testing (if either test was positive it was considered a positive screening); however, the esterase test was significantly less specific for bacteriuria than the nitrite test. Based on these results, the nitrite test was selected for use as the screening test. Rapid, direct identification and susceptibility tests on screen-positive urines showed 97% correlation with standard testing methods. Significant positive urines processed in this manner could be reported with quantitation, identification, and susceptibility results within 24 hr.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>3510805</pmid><doi>10.1016/0732-8893(86)90051-9</doi><tpages>7</tpages></addata></record> |
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| ispartof | Diagnostic microbiology and infectious disease, 1986, Vol.4 (1), p.11-17 |
| issn | 0732-8893 1879-0070 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Anti-Bacterial Agents - pharmacology Bacteria - drug effects Bacteriological methods and techniques used in bacteriology Bacteriology Bacteriuria - diagnosis Bacteriuria - microbiology Biological and medical sciences Child Escherichia coli - isolation & purification Esterases - analysis Female Fundamental and applied biological sciences. Psychology Humans In Vitro Techniques Leukocytes - enzymology Microbial Sensitivity Tests Microbiology Nitrites - urine Reagent Kits, Diagnostic Staphylococcus - isolation & purification Streptococcus - isolation & purification |
| title | Rapid screening and microbiologic processing of pediatric urine specimens |
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