Major core protein VP7 of Australian bluetongue virus serotype 15: sequence and antigenicity divergence from other BTV serotypes
CSIRO Australian Animal Health Laboratory, P.O. Bag 24, Geelong, Victoria 3220, Australia Full-length cDNA of the RNA genome segment coding for the major core protein VP7 of Australian bluetongue virus serotype 15 (BTV-15) has been isolated by reverse transcription-PCR cloning. Comparative analysis...
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| Veröffentlicht in: | Journal of general virology 1994-09, Vol.75 (9), p.2421-2425 |
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| creator | Wang, Lin-Fa Kattenbelt, Jacki A Gould, Allan R Pritchard, Lindsay I Crameri, Gary S Eaton, Bryan T |
| description | CSIRO Australian Animal Health Laboratory, P.O. Bag 24, Geelong, Victoria 3220, Australia
Full-length cDNA of the RNA genome segment coding for the major core protein VP7 of Australian bluetongue virus serotype 15 (BTV-15) has been isolated by reverse transcription-PCR cloning. Comparative analysis indicated that the BTV-15 VP7 sequence had diverged significantly from that of other members of the BTV serogroup. At the amino acid level, BTV-15 VP7 exhibited sequence identities of 80 to 84% with VP7 molecules of other serotypes, significantly lower than the sequence identities of between 93 and 100% observed among other serotypes characterized to date. This was consistent with previous observations that there were significant immunological differences between BTV-15 and other BTV serotypes and that monoclonal antibodies raised against BTV-1 VP7 failed to react with BTV-15 VP7. Recombinant BTV-15 VP7 protein produced from Escherichia coli was largely insoluble, but maintained its immunogenicity. Polyclonal mouse sera raised against the recombinant VP7 protein reacted strongly with VP7 of BTV-15, but weakly with that of BTV-1.
Received 21 January 1994;
accepted 19 April 1994. |
| doi_str_mv | 10.1099/0022-1317-75-9-2421 |
| format | Article |
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Full-length cDNA of the RNA genome segment coding for the major core protein VP7 of Australian bluetongue virus serotype 15 (BTV-15) has been isolated by reverse transcription-PCR cloning. Comparative analysis indicated that the BTV-15 VP7 sequence had diverged significantly from that of other members of the BTV serogroup. At the amino acid level, BTV-15 VP7 exhibited sequence identities of 80 to 84% with VP7 molecules of other serotypes, significantly lower than the sequence identities of between 93 and 100% observed among other serotypes characterized to date. This was consistent with previous observations that there were significant immunological differences between BTV-15 and other BTV serotypes and that monoclonal antibodies raised against BTV-1 VP7 failed to react with BTV-15 VP7. Recombinant BTV-15 VP7 protein produced from Escherichia coli was largely insoluble, but maintained its immunogenicity. Polyclonal mouse sera raised against the recombinant VP7 protein reacted strongly with VP7 of BTV-15, but weakly with that of BTV-1.
Received 21 January 1994;
accepted 19 April 1994.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-75-9-2421</identifier><identifier>PMID: 8077943</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Viral ; Antigens, Viral - chemistry ; Antigens, Viral - genetics ; Antigens, Viral - immunology ; Australia ; Base Sequence ; Biological and medical sciences ; bluetongue virus ; Bluetongue virus - classification ; Bluetongue virus - genetics ; Capsid - chemistry ; Capsid - genetics ; Capsid - immunology ; Capsid Proteins ; DNA Primers ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; Genome, Viral ; Mice ; Microbiology ; Molecular Sequence Data ; Phylogeny ; Polymerase Chain Reaction - methods ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Sequence Homology, Amino Acid ; Serotyping ; South Africa ; Virology</subject><ispartof>Journal of general virology, 1994-09, Vol.75 (9), p.2421-2425</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-f42439e030dbfb3f38c5fab366d0d522aff3cb9bbc7896f3b5ea2e3d6d8557223</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4224782$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8077943$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Lin-Fa</creatorcontrib><creatorcontrib>Kattenbelt, Jacki A</creatorcontrib><creatorcontrib>Gould, Allan R</creatorcontrib><creatorcontrib>Pritchard, Lindsay I</creatorcontrib><creatorcontrib>Crameri, Gary S</creatorcontrib><creatorcontrib>Eaton, Bryan T</creatorcontrib><title>Major core protein VP7 of Australian bluetongue virus serotype 15: sequence and antigenicity divergence from other BTV serotypes</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>CSIRO Australian Animal Health Laboratory, P.O. Bag 24, Geelong, Victoria 3220, Australia
Full-length cDNA of the RNA genome segment coding for the major core protein VP7 of Australian bluetongue virus serotype 15 (BTV-15) has been isolated by reverse transcription-PCR cloning. Comparative analysis indicated that the BTV-15 VP7 sequence had diverged significantly from that of other members of the BTV serogroup. At the amino acid level, BTV-15 VP7 exhibited sequence identities of 80 to 84% with VP7 molecules of other serotypes, significantly lower than the sequence identities of between 93 and 100% observed among other serotypes characterized to date. This was consistent with previous observations that there were significant immunological differences between BTV-15 and other BTV serotypes and that monoclonal antibodies raised against BTV-1 VP7 failed to react with BTV-15 VP7. Recombinant BTV-15 VP7 protein produced from Escherichia coli was largely insoluble, but maintained its immunogenicity. Polyclonal mouse sera raised against the recombinant VP7 protein reacted strongly with VP7 of BTV-15, but weakly with that of BTV-1.
Received 21 January 1994;
accepted 19 April 1994.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Viral</subject><subject>Antigens, Viral - chemistry</subject><subject>Antigens, Viral - genetics</subject><subject>Antigens, Viral - immunology</subject><subject>Australia</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>bluetongue virus</subject><subject>Bluetongue virus - classification</subject><subject>Bluetongue virus - genetics</subject><subject>Capsid - chemistry</subject><subject>Capsid - genetics</subject><subject>Capsid - immunology</subject><subject>Capsid Proteins</subject><subject>DNA Primers</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>Genome, Viral</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Sequence Homology, Amino Acid</subject><subject>Serotyping</subject><subject>South Africa</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKtPCL0BIXiDUTcBvJ-xKxUsqgkXp1rKd6xlXmXiwk6LZ8dNxmNGwZGFZ1vnu9b3nIPSCkjeUdN1bQhhrKKe60bLpGiYYfYRWVCjZsKo_RqsT8RSdl3JPCBVC6jN01hKtO8FX6PdXe58y9ikD3uU0QRzx3XeNU8BXc5myHaIdsRtmmNK4ngE_xDwXXKCy-x1gKt_Vx88ZRg_Yjn09U1zDGH2c9riPD5DXf7WQ0xanaQMZv7-9OzUoz9CTYIcCz4_3Bfrx8cPt9efm5tunL9dXN40XvJ2aIJjgHRBOehccD7z1MljHlepJLxmzIXDvOue8bjsVuJNgGfBe9a2UmjF-gV4f-tYt67hlMttYPAyDHSHNxWilWsW4_i9IlWKSt7KC_AD6nErJEMwux63Ne0OJWQIyi_1msd9oaTqzBFSrXh7bz24L_anmmEjVXx11W7wdQrajj-WECcaEbpd1Lg_YJq43v2IGU23exjqKi8nUkP79-AezF6hX</recordid><startdate>19940901</startdate><enddate>19940901</enddate><creator>Wang, Lin-Fa</creator><creator>Kattenbelt, Jacki A</creator><creator>Gould, Allan R</creator><creator>Pritchard, Lindsay I</creator><creator>Crameri, Gary S</creator><creator>Eaton, Bryan T</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19940901</creationdate><title>Major core protein VP7 of Australian bluetongue virus serotype 15: sequence and antigenicity divergence from other BTV serotypes</title><author>Wang, Lin-Fa ; Kattenbelt, Jacki A ; Gould, Allan R ; Pritchard, Lindsay I ; Crameri, Gary S ; Eaton, Bryan T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-f42439e030dbfb3f38c5fab366d0d522aff3cb9bbc7896f3b5ea2e3d6d8557223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Viral</topic><topic>Antigens, Viral - chemistry</topic><topic>Antigens, Viral - genetics</topic><topic>Antigens, Viral - immunology</topic><topic>Australia</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>bluetongue virus</topic><topic>Bluetongue virus - classification</topic><topic>Bluetongue virus - genetics</topic><topic>Capsid - chemistry</topic><topic>Capsid - genetics</topic><topic>Capsid - immunology</topic><topic>Capsid Proteins</topic><topic>DNA Primers</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>Genome, Viral</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Sequence Homology, Amino Acid</topic><topic>Serotyping</topic><topic>South Africa</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Lin-Fa</creatorcontrib><creatorcontrib>Kattenbelt, Jacki A</creatorcontrib><creatorcontrib>Gould, Allan R</creatorcontrib><creatorcontrib>Pritchard, Lindsay I</creatorcontrib><creatorcontrib>Crameri, Gary S</creatorcontrib><creatorcontrib>Eaton, Bryan T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Lin-Fa</au><au>Kattenbelt, Jacki A</au><au>Gould, Allan R</au><au>Pritchard, Lindsay I</au><au>Crameri, Gary S</au><au>Eaton, Bryan T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Major core protein VP7 of Australian bluetongue virus serotype 15: sequence and antigenicity divergence from other BTV serotypes</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1994-09-01</date><risdate>1994</risdate><volume>75</volume><issue>9</issue><spage>2421</spage><epage>2425</epage><pages>2421-2425</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>CSIRO Australian Animal Health Laboratory, P.O. Bag 24, Geelong, Victoria 3220, Australia
Full-length cDNA of the RNA genome segment coding for the major core protein VP7 of Australian bluetongue virus serotype 15 (BTV-15) has been isolated by reverse transcription-PCR cloning. Comparative analysis indicated that the BTV-15 VP7 sequence had diverged significantly from that of other members of the BTV serogroup. At the amino acid level, BTV-15 VP7 exhibited sequence identities of 80 to 84% with VP7 molecules of other serotypes, significantly lower than the sequence identities of between 93 and 100% observed among other serotypes characterized to date. This was consistent with previous observations that there were significant immunological differences between BTV-15 and other BTV serotypes and that monoclonal antibodies raised against BTV-1 VP7 failed to react with BTV-15 VP7. Recombinant BTV-15 VP7 protein produced from Escherichia coli was largely insoluble, but maintained its immunogenicity. Polyclonal mouse sera raised against the recombinant VP7 protein reacted strongly with VP7 of BTV-15, but weakly with that of BTV-1.
Received 21 January 1994;
accepted 19 April 1994.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>8077943</pmid><doi>10.1099/0022-1317-75-9-2421</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1994-09, Vol.75 (9), p.2421-2425 |
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| language | eng |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Antibodies, Viral Antigens, Viral - chemistry Antigens, Viral - genetics Antigens, Viral - immunology Australia Base Sequence Biological and medical sciences bluetongue virus Bluetongue virus - classification Bluetongue virus - genetics Capsid - chemistry Capsid - genetics Capsid - immunology Capsid Proteins DNA Primers Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Genetic Variation Genome, Viral Mice Microbiology Molecular Sequence Data Phylogeny Polymerase Chain Reaction - methods Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Sequence Homology, Amino Acid Serotyping South Africa Virology |
| title | Major core protein VP7 of Australian bluetongue virus serotype 15: sequence and antigenicity divergence from other BTV serotypes |
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