Spacer promoters are essential for efficient enhancement of X. laevis ribosomal transcription
The X. laevis ribosomal DNA spacer contains duplicated RNA polymerase I ”spacer promoters“ and an array of repeated 60 81 bp promoter-related sequences. The latter have been shown to enhance transcription from a 40S preribosomal RNA promoter in cis. Here we present evidence that at least one spacer...
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| Veröffentlicht in: | Cell 1986-01, Vol.44 (2), p.313-318 |
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| container_title | Cell |
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| creator | De Winter, Ronald F.J. Moss, Tom |
| description | The X. laevis ribosomal DNA spacer contains duplicated RNA polymerase I ”spacer promoters“ and an array of repeated
60
81
bp promoter-related sequences. The latter have been shown to enhance transcription from a 40S preribosomal RNA promoter in
cis. Here we present evidence that at least one spacer promoter is also necessary for efficient enhancement. Deletion of the spacer promoter sequences in a construct carrying only one such promoter reduces 40S RNA transcription to approximately 10% of wild type. This effect apparently is caused by inactivation of the spacer promoter, since mutants in which 4–177 bp of the spacer promoter and adjacent sequences are deleted are functionally equivalent. Spacer promoters and
60
80
bp arrays therefore probably act together to enhance 40S pre-RNA transcription in X. laevis. |
| doi_str_mv | 10.1016/0092-8674(86)90765-8 |
| format | Article |
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60
81
bp promoter-related sequences. The latter have been shown to enhance transcription from a 40S preribosomal RNA promoter in
cis. Here we present evidence that at least one spacer promoter is also necessary for efficient enhancement. Deletion of the spacer promoter sequences in a construct carrying only one such promoter reduces 40S RNA transcription to approximately 10% of wild type. This effect apparently is caused by inactivation of the spacer promoter, since mutants in which 4–177 bp of the spacer promoter and adjacent sequences are deleted are functionally equivalent. Spacer promoters and
60
80
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60
81
bp promoter-related sequences. The latter have been shown to enhance transcription from a 40S preribosomal RNA promoter in
cis. Here we present evidence that at least one spacer promoter is also necessary for efficient enhancement. Deletion of the spacer promoter sequences in a construct carrying only one such promoter reduces 40S RNA transcription to approximately 10% of wild type. This effect apparently is caused by inactivation of the spacer promoter, since mutants in which 4–177 bp of the spacer promoter and adjacent sequences are deleted are functionally equivalent. Spacer promoters and
60
80
bp arrays therefore probably act together to enhance 40S pre-RNA transcription in X. laevis.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>DNA, Ribosomal - genetics</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutation</subject><subject>Other techniques and industries</subject><subject>Promoter Regions, Genetic</subject><subject>RNA, Ribosomal - genetics</subject><subject>Transcription Factors - genetics</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Xenopus laevis</subject><subject>Xenopus laevis - genetics</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9LHDEYh4O06Lr6DRRyKNIeRpPJ_0uhSK2C0EM99FJCJvOGpsxMtsms0G9vxl32WC9JyPu8P948QeiCkmtKqLwhxLSNlop_1PKTIUqKRh-hFSVGNZyq9h1aHZATdFrKH0KIFkIco2NmOKOtXKFfPzbOQ8abnMY0Qy7YZcBQCkxzdAMOKWMIIfpYLzBMv93kYVzOKeCf13hw8BwLzrFLJY21Yc5uKj7HzRzTdIbeBzcUON_va_R09_Xp9r55_P7t4fbLY-M5l3PjWedoG7QOwtNAQDPDfCe40oYRZTroe84CGAOdC7RV3AgvOuq97rWs9Bpd7WLrK_5uocx2jMXDMLgJ0rZYJaUWnLE3QcolFUSoCvId6HMqJUOwmxxHl_9ZSuxi3y5q7aK2LvbVvl0Gudznb7sR-kPTXnetf9jXXfFuCNWVj-WAacKI5uYtTBkpeU1co887DKra5wjZluWbPPQxg59tn-L_x30BRY-udQ</recordid><startdate>19860131</startdate><enddate>19860131</enddate><creator>De Winter, Ronald F.J.</creator><creator>Moss, Tom</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19860131</creationdate><title>Spacer promoters are essential for efficient enhancement of X. laevis ribosomal transcription</title><author>De Winter, Ronald F.J. ; Moss, Tom</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-c3ba12f88f5c1f0e8393cb547893079bedd43fe99ebaf127495c5b1cc8d86e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>DNA, Ribosomal - genetics</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutation</topic><topic>Other techniques and industries</topic><topic>Promoter Regions, Genetic</topic><topic>RNA, Ribosomal - genetics</topic><topic>Transcription Factors - genetics</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Xenopus laevis</topic><topic>Xenopus laevis - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Winter, Ronald F.J.</creatorcontrib><creatorcontrib>Moss, Tom</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Winter, Ronald F.J.</au><au>Moss, Tom</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spacer promoters are essential for efficient enhancement of X. laevis ribosomal transcription</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1986-01-31</date><risdate>1986</risdate><volume>44</volume><issue>2</issue><spage>313</spage><epage>318</epage><pages>313-318</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>The X. laevis ribosomal DNA spacer contains duplicated RNA polymerase I ”spacer promoters“ and an array of repeated
60
81
bp promoter-related sequences. The latter have been shown to enhance transcription from a 40S preribosomal RNA promoter in
cis. Here we present evidence that at least one spacer promoter is also necessary for efficient enhancement. Deletion of the spacer promoter sequences in a construct carrying only one such promoter reduces 40S RNA transcription to approximately 10% of wild type. This effect apparently is caused by inactivation of the spacer promoter, since mutants in which 4–177 bp of the spacer promoter and adjacent sequences are deleted are functionally equivalent. Spacer promoters and
60
80
bp arrays therefore probably act together to enhance 40S pre-RNA transcription in X. laevis.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>3943126</pmid><doi>10.1016/0092-8674(86)90765-8</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Cell, 1986-01, Vol.44 (2), p.313-318 |
| issn | 0092-8674 1097-4172 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76685433 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Applied sciences Biological and medical sciences Chromosome Mapping DNA, Ribosomal - genetics Exact sciences and technology Fundamental and applied biological sciences. Psychology Gene Expression Regulation Molecular and cellular biology Molecular genetics Mutation Other techniques and industries Promoter Regions, Genetic RNA, Ribosomal - genetics Transcription Factors - genetics Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Xenopus laevis Xenopus laevis - genetics |
| title | Spacer promoters are essential for efficient enhancement of X. laevis ribosomal transcription |
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