Significance and Clinical Relevance of the Detection of Herpes Simplex Virus DNA by the Polymerase Chain Reaction in Cerebrospinal Fluid from Patients with Presumed Encephalitis
The polymerase chain reaction (PCR) was used to detect DNA of herpes simplex virus (HSV) in 38 samples of cerebrospinal fluid (CSF) obtained from 22 patients (7 children and 15 adults) 1–20 days after the onset of encephalitis. The results were best with amplification on the pellet of the CSF-extrac...
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| Veröffentlicht in: | Clinical infectious diseases 1994-05, Vol.18 (5), p.744-749 |
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| creator | Guffond, Thierry Dewilde, Anny Lobert, Pierre-Emmanuel Caparros-Lefebvre, Dominique Hober, Didier Wattre, Pierre |
| description | The polymerase chain reaction (PCR) was used to detect DNA of herpes simplex virus (HSV) in 38 samples of cerebrospinal fluid (CSF) obtained from 22 patients (7 children and 15 adults) 1–20 days after the onset of encephalitis. The results were best with amplification on the pellet of the CSF-extracted DNA and with analysis of the amplified products by dot-blotting (sensitivity, 100%). A highly significant difference was evident in the χ2 test when PCR was compared with specific antigen detection or antibody evaluation (n = 19; χ2 = 7; sensitivity = 100% vs. 63%) or with interferon α determination (n = 20; χ2 = 11; sensitivity = 95% vs. 42%). PCR was positive as early as 1 day after onset of disease and was often the first test to become positive. The detection of HSV DNA by PCR is the most specific, rapid, and sensitive tool for early diagnosis and therapeutic management of acute HSV encephalitis. |
| doi_str_mv | 10.1093/clinids/18.5.744 |
| format | Article |
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The results were best with amplification on the pellet of the CSF-extracted DNA and with analysis of the amplified products by dot-blotting (sensitivity, 100%). A highly significant difference was evident in the χ2 test when PCR was compared with specific antigen detection or antibody evaluation (n = 19; χ2 = 7; sensitivity = 100% vs. 63%) or with interferon α determination (n = 20; χ2 = 11; sensitivity = 95% vs. 42%). PCR was positive as early as 1 day after onset of disease and was often the first test to become positive. The detection of HSV DNA by PCR is the most specific, rapid, and sensitive tool for early diagnosis and therapeutic management of acute HSV encephalitis.</description><identifier>ISSN: 1058-4838</identifier><identifier>EISSN: 1537-6591</identifier><identifier>DOI: 10.1093/clinids/18.5.744</identifier><identifier>PMID: 8075263</identifier><identifier>CODEN: CIDIEL</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Adult ; Aged ; Animals ; Antibodies ; Antibodies, Viral - blood ; Antibodies, Viral - cerebrospinal fluid ; Antigens ; Antigens, Viral - cerebrospinal fluid ; Base Sequence ; Biological and medical sciences ; Cattle ; Cell Line ; Central Nervous System Diseases - cerebrospinal fluid ; Central nervous system viral diseases ; Cerebrospinal fluid ; Cerebrospinal Fluid - chemistry ; Cerebrospinal Fluid - microbiology ; Child ; Child, Preschool ; Clinical Infectious Disease Articles ; DNA ; DNA Probes ; DNA, Viral - cerebrospinal fluid ; Encephalitis ; Encephalitis - cerebrospinal fluid ; Encephalitis - diagnosis ; Encephalitis - microbiology ; Genes, pol ; Herpes Simplex - cerebrospinal fluid ; Herpes Simplex - diagnosis ; Herpes Simplex - microbiology ; Herpes simplex encephalitis ; herpes simplex virus ; Human viral diseases ; Humans ; Infant ; Infectious diseases ; Interferon-alpha - biosynthesis ; Interferon-alpha - blood ; Interferon-alpha - cerebrospinal fluid ; Medical sciences ; Middle Aged ; Molecular Sequence Data ; Polymerase Chain Reaction ; Sensitivity and Specificity ; Simplexvirus ; Simplexvirus - genetics ; Simplexvirus - immunology ; Simplexvirus - isolation & purification ; Time Factors ; Viral diseases ; Viral diseases of the nervous system ; Viruses</subject><ispartof>Clinical infectious diseases, 1994-05, Vol.18 (5), p.744-749</ispartof><rights>Copyright 1994 The University of Chicago</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-7c66e933993f4e47a04481f5af9e63953f68e46030ac86db5e784a73f0ccb53c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4457796$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4457796$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3306218$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8075263$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guffond, Thierry</creatorcontrib><creatorcontrib>Dewilde, Anny</creatorcontrib><creatorcontrib>Lobert, Pierre-Emmanuel</creatorcontrib><creatorcontrib>Caparros-Lefebvre, Dominique</creatorcontrib><creatorcontrib>Hober, Didier</creatorcontrib><creatorcontrib>Wattre, Pierre</creatorcontrib><title>Significance and Clinical Relevance of the Detection of Herpes Simplex Virus DNA by the Polymerase Chain Reaction in Cerebrospinal Fluid from Patients with Presumed Encephalitis</title><title>Clinical infectious diseases</title><addtitle>Clinical Infectious Diseases</addtitle><description>The polymerase chain reaction (PCR) was used to detect DNA of herpes simplex virus (HSV) in 38 samples of cerebrospinal fluid (CSF) obtained from 22 patients (7 children and 15 adults) 1–20 days after the onset of encephalitis. The results were best with amplification on the pellet of the CSF-extracted DNA and with analysis of the amplified products by dot-blotting (sensitivity, 100%). A highly significant difference was evident in the χ2 test when PCR was compared with specific antigen detection or antibody evaluation (n = 19; χ2 = 7; sensitivity = 100% vs. 63%) or with interferon α determination (n = 20; χ2 = 11; sensitivity = 95% vs. 42%). PCR was positive as early as 1 day after onset of disease and was often the first test to become positive. The detection of HSV DNA by PCR is the most specific, rapid, and sensitive tool for early diagnosis and therapeutic management of acute HSV encephalitis.</description><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Antibodies, Viral - cerebrospinal fluid</subject><subject>Antigens</subject><subject>Antigens, Viral - cerebrospinal fluid</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell Line</subject><subject>Central Nervous System Diseases - cerebrospinal fluid</subject><subject>Central nervous system viral diseases</subject><subject>Cerebrospinal fluid</subject><subject>Cerebrospinal Fluid - chemistry</subject><subject>Cerebrospinal Fluid - microbiology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Clinical Infectious Disease Articles</subject><subject>DNA</subject><subject>DNA Probes</subject><subject>DNA, Viral - cerebrospinal fluid</subject><subject>Encephalitis</subject><subject>Encephalitis - cerebrospinal fluid</subject><subject>Encephalitis - diagnosis</subject><subject>Encephalitis - microbiology</subject><subject>Genes, pol</subject><subject>Herpes Simplex - cerebrospinal fluid</subject><subject>Herpes Simplex - diagnosis</subject><subject>Herpes Simplex - microbiology</subject><subject>Herpes simplex encephalitis</subject><subject>herpes simplex virus</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infant</subject><subject>Infectious diseases</subject><subject>Interferon-alpha - biosynthesis</subject><subject>Interferon-alpha - blood</subject><subject>Interferon-alpha - cerebrospinal fluid</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Sensitivity and Specificity</subject><subject>Simplexvirus</subject><subject>Simplexvirus - genetics</subject><subject>Simplexvirus - immunology</subject><subject>Simplexvirus - isolation & purification</subject><subject>Time Factors</subject><subject>Viral diseases</subject><subject>Viral diseases of the nervous system</subject><subject>Viruses</subject><issn>1058-4838</issn><issn>1537-6591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEUhUcIVEphzwIkLxC7Se34Octq2jSgCtIWEGJjOc41cZkXtqc0P4t_iNNE2bLylc93j-_1KYrXBE8IruipbXznV_GUqAmfSMaeFMeEU1kKXpGnucZclUxR9bx4EeMdxoQozI-KI4Ulnwp6XPy99T8777w1nQVkuhWqt5bWNOgGGrh_vO4dSmtA55DAJt9324s5hAEiuvXt0MAD-ubDGNH5pzO03DzCi77ZtBBMBFSvje-yndk157qGAMvQx8F3-aFZM_oVcqFv0cIkD12K6I9Pa7QIEMcWVugiTzGsTeOTjy-LZ840EV7tz5Pi6-ziSz0vrz5ffqjPrkpLpUyltEJARWlVUceASYMZU8Rx4yoQtOLUCQVMYIqNVWK15CAVM5I6bO2SU0tPivc73yH0v0eISbc-Wmga00E_Ri2FUIwK-V-QiEoqJUUG8Q60efUYwOkh-NaEjSZYb-PU-zg1UZrrHGduebv3Hpf5Iw4N-_yy_m6vm5gzcyHn5eMBoxSLKVEZe7PD7mLqw0FmjEtZbQcrd7KPCR4Osgm_dN5Pcj3__kNjfn39Uc2m-ob-A4NNxL4</recordid><startdate>19940501</startdate><enddate>19940501</enddate><creator>Guffond, Thierry</creator><creator>Dewilde, Anny</creator><creator>Lobert, Pierre-Emmanuel</creator><creator>Caparros-Lefebvre, Dominique</creator><creator>Hober, Didier</creator><creator>Wattre, Pierre</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19940501</creationdate><title>Significance and Clinical Relevance of the Detection of Herpes Simplex Virus DNA by the Polymerase Chain Reaction in Cerebrospinal Fluid from Patients with Presumed Encephalitis</title><author>Guffond, Thierry ; Dewilde, Anny ; Lobert, Pierre-Emmanuel ; Caparros-Lefebvre, Dominique ; Hober, Didier ; Wattre, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-7c66e933993f4e47a04481f5af9e63953f68e46030ac86db5e784a73f0ccb53c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Viral - blood</topic><topic>Antibodies, Viral - cerebrospinal fluid</topic><topic>Antigens</topic><topic>Antigens, Viral - cerebrospinal fluid</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cell Line</topic><topic>Central Nervous System Diseases - cerebrospinal fluid</topic><topic>Central nervous system viral diseases</topic><topic>Cerebrospinal fluid</topic><topic>Cerebrospinal Fluid - chemistry</topic><topic>Cerebrospinal Fluid - microbiology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Clinical Infectious Disease Articles</topic><topic>DNA</topic><topic>DNA Probes</topic><topic>DNA, Viral - cerebrospinal fluid</topic><topic>Encephalitis</topic><topic>Encephalitis - cerebrospinal fluid</topic><topic>Encephalitis - diagnosis</topic><topic>Encephalitis - microbiology</topic><topic>Genes, pol</topic><topic>Herpes Simplex - cerebrospinal fluid</topic><topic>Herpes Simplex - diagnosis</topic><topic>Herpes Simplex - microbiology</topic><topic>Herpes simplex encephalitis</topic><topic>herpes simplex virus</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infant</topic><topic>Infectious diseases</topic><topic>Interferon-alpha - biosynthesis</topic><topic>Interferon-alpha - blood</topic><topic>Interferon-alpha - cerebrospinal fluid</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>Sensitivity and Specificity</topic><topic>Simplexvirus</topic><topic>Simplexvirus - genetics</topic><topic>Simplexvirus - immunology</topic><topic>Simplexvirus - isolation & purification</topic><topic>Time Factors</topic><topic>Viral diseases</topic><topic>Viral diseases of the nervous system</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guffond, Thierry</creatorcontrib><creatorcontrib>Dewilde, Anny</creatorcontrib><creatorcontrib>Lobert, Pierre-Emmanuel</creatorcontrib><creatorcontrib>Caparros-Lefebvre, Dominique</creatorcontrib><creatorcontrib>Hober, Didier</creatorcontrib><creatorcontrib>Wattre, Pierre</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guffond, Thierry</au><au>Dewilde, Anny</au><au>Lobert, Pierre-Emmanuel</au><au>Caparros-Lefebvre, Dominique</au><au>Hober, Didier</au><au>Wattre, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Significance and Clinical Relevance of the Detection of Herpes Simplex Virus DNA by the Polymerase Chain Reaction in Cerebrospinal Fluid from Patients with Presumed Encephalitis</atitle><jtitle>Clinical infectious diseases</jtitle><addtitle>Clinical Infectious Diseases</addtitle><date>1994-05-01</date><risdate>1994</risdate><volume>18</volume><issue>5</issue><spage>744</spage><epage>749</epage><pages>744-749</pages><issn>1058-4838</issn><eissn>1537-6591</eissn><coden>CIDIEL</coden><abstract>The polymerase chain reaction (PCR) was used to detect DNA of herpes simplex virus (HSV) in 38 samples of cerebrospinal fluid (CSF) obtained from 22 patients (7 children and 15 adults) 1–20 days after the onset of encephalitis. The results were best with amplification on the pellet of the CSF-extracted DNA and with analysis of the amplified products by dot-blotting (sensitivity, 100%). A highly significant difference was evident in the χ2 test when PCR was compared with specific antigen detection or antibody evaluation (n = 19; χ2 = 7; sensitivity = 100% vs. 63%) or with interferon α determination (n = 20; χ2 = 11; sensitivity = 95% vs. 42%). PCR was positive as early as 1 day after onset of disease and was often the first test to become positive. The detection of HSV DNA by PCR is the most specific, rapid, and sensitive tool for early diagnosis and therapeutic management of acute HSV encephalitis.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>8075263</pmid><doi>10.1093/clinids/18.5.744</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Clinical infectious diseases, 1994-05, Vol.18 (5), p.744-749 |
| issn | 1058-4838 1537-6591 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; JSTOR Archive Collection A-Z Listing |
| subjects | Adult Aged Animals Antibodies Antibodies, Viral - blood Antibodies, Viral - cerebrospinal fluid Antigens Antigens, Viral - cerebrospinal fluid Base Sequence Biological and medical sciences Cattle Cell Line Central Nervous System Diseases - cerebrospinal fluid Central nervous system viral diseases Cerebrospinal fluid Cerebrospinal Fluid - chemistry Cerebrospinal Fluid - microbiology Child Child, Preschool Clinical Infectious Disease Articles DNA DNA Probes DNA, Viral - cerebrospinal fluid Encephalitis Encephalitis - cerebrospinal fluid Encephalitis - diagnosis Encephalitis - microbiology Genes, pol Herpes Simplex - cerebrospinal fluid Herpes Simplex - diagnosis Herpes Simplex - microbiology Herpes simplex encephalitis herpes simplex virus Human viral diseases Humans Infant Infectious diseases Interferon-alpha - biosynthesis Interferon-alpha - blood Interferon-alpha - cerebrospinal fluid Medical sciences Middle Aged Molecular Sequence Data Polymerase Chain Reaction Sensitivity and Specificity Simplexvirus Simplexvirus - genetics Simplexvirus - immunology Simplexvirus - isolation & purification Time Factors Viral diseases Viral diseases of the nervous system Viruses |
| title | Significance and Clinical Relevance of the Detection of Herpes Simplex Virus DNA by the Polymerase Chain Reaction in Cerebrospinal Fluid from Patients with Presumed Encephalitis |
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