Construction and Properties of Tn917-lac, a Transposon Derivative That Mediates Transcriptional Gene Fusions in Bacillus subtilis

A derivative of Tn917 was constructed, referred to as Tn917-lac, which is capable of generating fusions that connect the transcripts of Bacillus subtilis chromosomal genes to the coding sequence of the lacZ gene of Escherichia coli. Two independent insertions of Tn917-lac into the gltA gene and one...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1986-01, Vol.83 (1), p.140-144
Hauptverfasser:	Perkins, John B., Youngman, Philip J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus subtilis - genetics Bacteria beta-Galactosidase - genetics Biological and medical sciences Biotechnology Diploidy DNA DNA Transposable Elements DNA, Bacterial - genetics DNA, Recombinant Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene fusion Genes Genes, Bacterial Genetic engineering Genetic technics Genetic transposition Lac Operon Methods. Procedures. Technologies Nucleic Acid Hybridization Operons Plasmids Promoter Regions, Genetic Transcription, Genetic Transposons
Online-Zugang:	Volltext
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