In vitro metabolism of mevalonic acid in the bovine retina
: Bovine retinas were incubated with 3RS‐[5‐3H]‐ mevalonic acid under conditions similar to those previously shown to support opsin biosynthesis in vitro. TLC of the total lipids indicated the formation of numerous radiolabeled components, including sterols, hydrocarbons, and “fatty acid‐like materi...
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| Veröffentlicht in: | Journal of neurochemistry 1986-02, Vol.46 (2), p.448-460 |
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| container_title | Journal of neurochemistry |
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| creator | Fliesler, S.J Schroepfer, G.J. Jr |
| description | : Bovine retinas were incubated with 3RS‐[5‐3H]‐ mevalonic acid under conditions similar to those previously shown to support opsin biosynthesis in vitro. TLC of the total lipids indicated the formation of numerous radiolabeled components, including sterols, hydrocarbons, and “fatty acid‐like material.” The nonsaponifiable lipids were analyzed by TLC, GLC, and chromatography on columns of silicic acid‐Super Cel, silica gel G‐Super Cel‐silver nitrate, and alumina‐Super Cel‐silver nitrate. The major nonsaponifiable components had the Chromatographic properties of squalene and “methylated sterols” (i.e., C30, C29, and C28 monohydroxy sterols). Cholesterol represented no more than 1% of the total radioactivity in the nonsaponifiable lipid fraction. The “fatty acid‐like material” was derivatized with diazomethane, and the resulting methyl esters were analyzed by GLC before and after catalytic hydrogenation. The radioactivity did not correspond to the normal fatty acids endogenous to the retina, but rather had the Chromatographic properties of C15 and C20 isoprenoid acids. These results obtained with intact retinas are consistent with our previous observations concerning mevalonic acid metabolism in cell‐free homogenates of bovine retinas. |
| doi_str_mv | 10.1111/j.1471-4159.1986.tb12989.x |
| format | Article |
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The “fatty acid‐like material” was derivatized with diazomethane, and the resulting methyl esters were analyzed by GLC before and after catalytic hydrogenation. The radioactivity did not correspond to the normal fatty acids endogenous to the retina, but rather had the Chromatographic properties of C15 and C20 isoprenoid acids. These results obtained with intact retinas are consistent with our previous observations concerning mevalonic acid metabolism in cell‐free homogenates of bovine retinas.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1986.tb12989.x</identifier><identifier>PMID: 3941316</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; Cholesterol ; Chromatography, Gas ; Chromatography, Thin Layer ; Eye and associated structures. 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Jr</creatorcontrib><title>In vitro metabolism of mevalonic acid in the bovine retina</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: Bovine retinas were incubated with 3RS‐[5‐3H]‐ mevalonic acid under conditions similar to those previously shown to support opsin biosynthesis in vitro. TLC of the total lipids indicated the formation of numerous radiolabeled components, including sterols, hydrocarbons, and “fatty acid‐like material.” The nonsaponifiable lipids were analyzed by TLC, GLC, and chromatography on columns of silicic acid‐Super Cel, silica gel G‐Super Cel‐silver nitrate, and alumina‐Super Cel‐silver nitrate. The major nonsaponifiable components had the Chromatographic properties of squalene and “methylated sterols” (i.e., C30, C29, and C28 monohydroxy sterols). Cholesterol represented no more than 1% of the total radioactivity in the nonsaponifiable lipid fraction. The “fatty acid‐like material” was derivatized with diazomethane, and the resulting methyl esters were analyzed by GLC before and after catalytic hydrogenation. The radioactivity did not correspond to the normal fatty acids endogenous to the retina, but rather had the Chromatographic properties of C15 and C20 isoprenoid acids. These results obtained with intact retinas are consistent with our previous observations concerning mevalonic acid metabolism in cell‐free homogenates of bovine retinas.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cholesterol</subject><subject>Chromatography, Gas</subject><subject>Chromatography, Thin Layer</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>in vitro</subject><subject>In Vitro Techniques</subject><subject>Isopren‐oids</subject><subject>Lipids</subject><subject>metabolism</subject><subject>Mevalonic acid</subject><subject>Mevalonic Acid - metabolism</subject><subject>Retina</subject><subject>Retina - metabolism</subject><subject>Sterol biosynthesis</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkF1LwzAYhYMoc05_glhEvGvNV9NmF4IMPxl6obsOaZpqRtvMpJvu35uysntz8xLOec95eQC4RDBB4d0sE0QzFFOU8gTxnCVdgTDPefJ7AMZ76RCMIcQ4JpDiY3Di_RJCxChDIzAinCKC2BhMn9toYzpno0Z3srC18U1kq_DbyNq2RkVSmTIybdR96aiwG9PqyOnOtPIUHFWy9vpsmBOweLj_mD3F87fH59ndPFYUkjwmUjNFCM0zlYZWphgvYEkJzPojdckKxgksEVdVgTPIMqhzhAmueMpIqisyAde73JWz32vtO9EYr3Rdy1bbtRcZYznBOQ3G6c6onPXe6UqsnGmk2woERQ9OLEVPR_TFogcnBnDiNyyfDy3rotHlfnUgFfSrQZdeybpyslXG720ZzwJrHmy3O9uPqfX2HweIl9cZpXkIuNgFVNIK-elCx-IdQ0QgopRzkpI_5fuRVQ</recordid><startdate>198602</startdate><enddate>198602</enddate><creator>Fliesler, S.J</creator><creator>Schroepfer, G.J. Jr</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198602</creationdate><title>In vitro metabolism of mevalonic acid in the bovine retina</title><author>Fliesler, S.J ; Schroepfer, G.J. Jr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4038-3ae6c33487c59416c69b0d43074159ed6b6930d19cfb270670e81232f95635ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cholesterol</topic><topic>Chromatography, Gas</topic><topic>Chromatography, Thin Layer</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>in vitro</topic><topic>In Vitro Techniques</topic><topic>Isopren‐oids</topic><topic>Lipids</topic><topic>metabolism</topic><topic>Mevalonic acid</topic><topic>Mevalonic Acid - metabolism</topic><topic>Retina</topic><topic>Retina - metabolism</topic><topic>Sterol biosynthesis</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fliesler, S.J</creatorcontrib><creatorcontrib>Schroepfer, G.J. Jr</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fliesler, S.J</au><au>Schroepfer, G.J. Jr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro metabolism of mevalonic acid in the bovine retina</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1986-02</date><risdate>1986</risdate><volume>46</volume><issue>2</issue><spage>448</spage><epage>460</epage><pages>448-460</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: Bovine retinas were incubated with 3RS‐[5‐3H]‐ mevalonic acid under conditions similar to those previously shown to support opsin biosynthesis in vitro. TLC of the total lipids indicated the formation of numerous radiolabeled components, including sterols, hydrocarbons, and “fatty acid‐like material.” The nonsaponifiable lipids were analyzed by TLC, GLC, and chromatography on columns of silicic acid‐Super Cel, silica gel G‐Super Cel‐silver nitrate, and alumina‐Super Cel‐silver nitrate. The major nonsaponifiable components had the Chromatographic properties of squalene and “methylated sterols” (i.e., C30, C29, and C28 monohydroxy sterols). Cholesterol represented no more than 1% of the total radioactivity in the nonsaponifiable lipid fraction. The “fatty acid‐like material” was derivatized with diazomethane, and the resulting methyl esters were analyzed by GLC before and after catalytic hydrogenation. The radioactivity did not correspond to the normal fatty acids endogenous to the retina, but rather had the Chromatographic properties of C15 and C20 isoprenoid acids. These results obtained with intact retinas are consistent with our previous observations concerning mevalonic acid metabolism in cell‐free homogenates of bovine retinas.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>3941316</pmid><doi>10.1111/j.1471-4159.1986.tb12989.x</doi><tpages>13</tpages></addata></record> |
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| ispartof | Journal of neurochemistry, 1986-02, Vol.46 (2), p.448-460 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biological and medical sciences Cattle Cholesterol Chromatography, Gas Chromatography, Thin Layer Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology in vitro In Vitro Techniques Isopren‐oids Lipids metabolism Mevalonic acid Mevalonic Acid - metabolism Retina Retina - metabolism Sterol biosynthesis Vertebrates: nervous system and sense organs |
| title | In vitro metabolism of mevalonic acid in the bovine retina |
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