Growth, morphology, and morphometry of human hypertrophic prostate cells treated with suramin in vitro
This work studies the effects of suramin on the growth and morphology of cell strain U285, obtained from human prostate hypertrophic tissue and cultured in vitro. The FRAME cytotoxicity test was performed to evaluate the inhibition of growth induced by suramin. Cells were exposed to suramin at the t...
Gespeichert in:
| Veröffentlicht in: | The Prostate 1994-09, Vol.25 (3), p.117-124 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 124 |
|---|---|
| container_issue | 3 |
| container_start_page | 117 |
| container_title | The Prostate |
| container_volume | 25 |
| creator | De Angeli, Sergio Fandella, Andrea Conconi, Maria Teresa Anselmo, Giuseppe Parnigotto, Pier Paolo |
| description | This work studies the effects of suramin on the growth and morphology of cell strain U285, obtained from human prostate hypertrophic tissue and cultured in vitro. The FRAME cytotoxicity test was performed to evaluate the inhibition of growth induced by suramin. Cells were exposed to suramin at the time of seeding and 24 hours later; neutral red was added with and without suramin. An optical microscope connected to a computer‐aided system and a scanning electron microscope were used to study morphological changes induced by suramin. Growth inhibition depends on drug concentration and exposure period. Moreover, the effect of suramin on neutral red uptake is reversible. Suramin 1,000 μM causes the cells to become spheroid, and they fail to form a monolayer. Our data indicate that the addition of suramin during the lag phase decreases the rate of cell proliferation. © 1994 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/pros.2990250302 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76672849</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76672849</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4112-4171216e00049f0e2870be05c49f1844594e74ff18089fdbabd92280b43dc97b3</originalsourceid><addsrcrecordid>eNqFUE1r3DAQFaEl3aQ591TQofQUJyNZsix6KqHZBEJT-kEgFyHbcq3WX5XkbPzvo2XNlpwCgtFj3pt5bxB6R-CMANDz0Q3-jEoJlEMK9ACtCEiRADD-Cq2ACkgYScUbdOT9H4CoAXqIDgWnwAVfoXrthk1oTnE3uLEZ2uH3fIp1Xy24M8HNeKhxM3W6x808GhfcMDa2xNvVQQeDS9O2HgdnIqjwxoYG-8npzvY4vgcbBW_R61q33pws9Rj9uvzy8-IqubldX198vklKRgiNVgWhJDPRJZM1GJoLKAzwMiKSM8YlM4LV8Q-5rKtCF5WkNIeCpVUpRZEeo4-7udHcv8n4oDrrt_50b4bJK5FlguZMRuL5jljGFN6ZWo3OdtrNioDaXlZt46n_l42K98voqehMtecvp4z9D0tf-1K3tdN9af2exijNYoRI-7SjbWxr5pe2qm_fb388M5Hs1NYH87hXa_dXZSIVXN19Xau7-5yv5f2VgvQJwkei5Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76672849</pqid></control><display><type>article</type><title>Growth, morphology, and morphometry of human hypertrophic prostate cells treated with suramin in vitro</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>De Angeli, Sergio ; Fandella, Andrea ; Conconi, Maria Teresa ; Anselmo, Giuseppe ; Parnigotto, Pier Paolo</creator><creatorcontrib>De Angeli, Sergio ; Fandella, Andrea ; Conconi, Maria Teresa ; Anselmo, Giuseppe ; Parnigotto, Pier Paolo</creatorcontrib><description>This work studies the effects of suramin on the growth and morphology of cell strain U285, obtained from human prostate hypertrophic tissue and cultured in vitro. The FRAME cytotoxicity test was performed to evaluate the inhibition of growth induced by suramin. Cells were exposed to suramin at the time of seeding and 24 hours later; neutral red was added with and without suramin. An optical microscope connected to a computer‐aided system and a scanning electron microscope were used to study morphological changes induced by suramin. Growth inhibition depends on drug concentration and exposure period. Moreover, the effect of suramin on neutral red uptake is reversible. Suramin 1,000 μM causes the cells to become spheroid, and they fail to form a monolayer. Our data indicate that the addition of suramin during the lag phase decreases the rate of cell proliferation. © 1994 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/pros.2990250302</identifier><identifier>PMID: 7520575</identifier><identifier>CODEN: PRSTDS</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Biological and medical sciences ; Cell Division - drug effects ; Cell Size - drug effects ; Cells, Cultured - drug effects ; FRAME cytotoxicity test ; human hypertrophic prostate cell strain U285 ; Humans ; image analysis ; lag phase ; log phase ; Male ; Medical sciences ; Microscopy, Electron, Scanning ; Nephrology. Urinary tract diseases ; Prostatic Hyperplasia - drug therapy ; Prostatic Hyperplasia - pathology ; scanning electron microscopy ; Suramin - pharmacology ; Tumors of the urinary system ; Urinary tract. Prostate gland</subject><ispartof>The Prostate, 1994-09, Vol.25 (3), p.117-124</ispartof><rights>Copyright © 1994 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4112-4171216e00049f0e2870be05c49f1844594e74ff18089fdbabd92280b43dc97b3</citedby><cites>FETCH-LOGICAL-c4112-4171216e00049f0e2870be05c49f1844594e74ff18089fdbabd92280b43dc97b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpros.2990250302$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpros.2990250302$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4226184$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7520575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Angeli, Sergio</creatorcontrib><creatorcontrib>Fandella, Andrea</creatorcontrib><creatorcontrib>Conconi, Maria Teresa</creatorcontrib><creatorcontrib>Anselmo, Giuseppe</creatorcontrib><creatorcontrib>Parnigotto, Pier Paolo</creatorcontrib><title>Growth, morphology, and morphometry of human hypertrophic prostate cells treated with suramin in vitro</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>This work studies the effects of suramin on the growth and morphology of cell strain U285, obtained from human prostate hypertrophic tissue and cultured in vitro. The FRAME cytotoxicity test was performed to evaluate the inhibition of growth induced by suramin. Cells were exposed to suramin at the time of seeding and 24 hours later; neutral red was added with and without suramin. An optical microscope connected to a computer‐aided system and a scanning electron microscope were used to study morphological changes induced by suramin. Growth inhibition depends on drug concentration and exposure period. Moreover, the effect of suramin on neutral red uptake is reversible. Suramin 1,000 μM causes the cells to become spheroid, and they fail to form a monolayer. Our data indicate that the addition of suramin during the lag phase decreases the rate of cell proliferation. © 1994 Wiley‐Liss, Inc.</description><subject>Biological and medical sciences</subject><subject>Cell Division - drug effects</subject><subject>Cell Size - drug effects</subject><subject>Cells, Cultured - drug effects</subject><subject>FRAME cytotoxicity test</subject><subject>human hypertrophic prostate cell strain U285</subject><subject>Humans</subject><subject>image analysis</subject><subject>lag phase</subject><subject>log phase</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Prostatic Hyperplasia - drug therapy</subject><subject>Prostatic Hyperplasia - pathology</subject><subject>scanning electron microscopy</subject><subject>Suramin - pharmacology</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. Prostate gland</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUE1r3DAQFaEl3aQ591TQofQUJyNZsix6KqHZBEJT-kEgFyHbcq3WX5XkbPzvo2XNlpwCgtFj3pt5bxB6R-CMANDz0Q3-jEoJlEMK9ACtCEiRADD-Cq2ACkgYScUbdOT9H4CoAXqIDgWnwAVfoXrthk1oTnE3uLEZ2uH3fIp1Xy24M8HNeKhxM3W6x808GhfcMDa2xNvVQQeDS9O2HgdnIqjwxoYG-8npzvY4vgcbBW_R61q33pws9Rj9uvzy8-IqubldX198vklKRgiNVgWhJDPRJZM1GJoLKAzwMiKSM8YlM4LV8Q-5rKtCF5WkNIeCpVUpRZEeo4-7udHcv8n4oDrrt_50b4bJK5FlguZMRuL5jljGFN6ZWo3OdtrNioDaXlZt46n_l42K98voqehMtecvp4z9D0tf-1K3tdN9af2exijNYoRI-7SjbWxr5pe2qm_fb388M5Hs1NYH87hXa_dXZSIVXN19Xau7-5yv5f2VgvQJwkei5Q</recordid><startdate>199409</startdate><enddate>199409</enddate><creator>De Angeli, Sergio</creator><creator>Fandella, Andrea</creator><creator>Conconi, Maria Teresa</creator><creator>Anselmo, Giuseppe</creator><creator>Parnigotto, Pier Paolo</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199409</creationdate><title>Growth, morphology, and morphometry of human hypertrophic prostate cells treated with suramin in vitro</title><author>De Angeli, Sergio ; Fandella, Andrea ; Conconi, Maria Teresa ; Anselmo, Giuseppe ; Parnigotto, Pier Paolo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4112-4171216e00049f0e2870be05c49f1844594e74ff18089fdbabd92280b43dc97b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Cell Size - drug effects</topic><topic>Cells, Cultured - drug effects</topic><topic>FRAME cytotoxicity test</topic><topic>human hypertrophic prostate cell strain U285</topic><topic>Humans</topic><topic>image analysis</topic><topic>lag phase</topic><topic>log phase</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microscopy, Electron, Scanning</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Prostatic Hyperplasia - drug therapy</topic><topic>Prostatic Hyperplasia - pathology</topic><topic>scanning electron microscopy</topic><topic>Suramin - pharmacology</topic><topic>Tumors of the urinary system</topic><topic>Urinary tract. Prostate gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Angeli, Sergio</creatorcontrib><creatorcontrib>Fandella, Andrea</creatorcontrib><creatorcontrib>Conconi, Maria Teresa</creatorcontrib><creatorcontrib>Anselmo, Giuseppe</creatorcontrib><creatorcontrib>Parnigotto, Pier Paolo</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Angeli, Sergio</au><au>Fandella, Andrea</au><au>Conconi, Maria Teresa</au><au>Anselmo, Giuseppe</au><au>Parnigotto, Pier Paolo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth, morphology, and morphometry of human hypertrophic prostate cells treated with suramin in vitro</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>1994-09</date><risdate>1994</risdate><volume>25</volume><issue>3</issue><spage>117</spage><epage>124</epage><pages>117-124</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><coden>PRSTDS</coden><abstract>This work studies the effects of suramin on the growth and morphology of cell strain U285, obtained from human prostate hypertrophic tissue and cultured in vitro. The FRAME cytotoxicity test was performed to evaluate the inhibition of growth induced by suramin. Cells were exposed to suramin at the time of seeding and 24 hours later; neutral red was added with and without suramin. An optical microscope connected to a computer‐aided system and a scanning electron microscope were used to study morphological changes induced by suramin. Growth inhibition depends on drug concentration and exposure period. Moreover, the effect of suramin on neutral red uptake is reversible. Suramin 1,000 μM causes the cells to become spheroid, and they fail to form a monolayer. Our data indicate that the addition of suramin during the lag phase decreases the rate of cell proliferation. © 1994 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7520575</pmid><doi>10.1002/pros.2990250302</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0270-4137 |
| ispartof | The Prostate, 1994-09, Vol.25 (3), p.117-124 |
| issn | 0270-4137 1097-0045 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76672849 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences Cell Division - drug effects Cell Size - drug effects Cells, Cultured - drug effects FRAME cytotoxicity test human hypertrophic prostate cell strain U285 Humans image analysis lag phase log phase Male Medical sciences Microscopy, Electron, Scanning Nephrology. Urinary tract diseases Prostatic Hyperplasia - drug therapy Prostatic Hyperplasia - pathology scanning electron microscopy Suramin - pharmacology Tumors of the urinary system Urinary tract. Prostate gland |
| title | Growth, morphology, and morphometry of human hypertrophic prostate cells treated with suramin in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T08%3A24%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Growth,%20morphology,%20and%20morphometry%20of%20human%20hypertrophic%20prostate%20cells%20treated%20with%20suramin%20in%20vitro&rft.jtitle=The%20Prostate&rft.au=De%20Angeli,%20Sergio&rft.date=1994-09&rft.volume=25&rft.issue=3&rft.spage=117&rft.epage=124&rft.pages=117-124&rft.issn=0270-4137&rft.eissn=1097-0045&rft.coden=PRSTDS&rft_id=info:doi/10.1002/pros.2990250302&rft_dat=%3Cproquest_cross%3E76672849%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76672849&rft_id=info:pmid/7520575&rfr_iscdi=true |