Preparation and antigenic properties of digitoxin-bovine serum albumin conjugates linked at the digitoxose C-3' and C-3 positions
In order to obtain specific antisera to digitoxin, four new types of hapten-bovine serum albumin (BSA) conjugates were synthesized from digitoxin. The haptens were linked to the carrier protein through hemisuccinate and hemisuccinylglycine bridges at the C-3' and C-3" positions in the digi...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 1994, Vol.17 (4), p.467-471 |
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| creator | FUJII, Y IKEDA, Y FUJII, M YAMAZAKI, M |
| description | In order to obtain specific antisera to digitoxin, four new types of hapten-bovine serum albumin (BSA) conjugates were synthesized from digitoxin. The haptens were linked to the carrier protein through hemisuccinate and hemisuccinylglycine bridges at the C-3' and C-3" positions in the digitoxose chain. The antisera were prepared by immunizing rabbits with each digitoxin-BSA conjugate and the properties of the antisera were investigated by RIA with 3H-labeled digitoxin. Among these antisera, the antiserum raised against digitoxin 3'-hemisuccinate-BSA conjugate possessed high specificity for digitoxin, exhibiting only minor cross-reactions with digitoxigenin bisdigitoxoside (4.0%), dihydrodigitoxin (2.8%), digoxin (2.4%), digitoxigenin monodigitoxoside (0.23%) and digitoxigenin (< 0.05%). |
| doi_str_mv | 10.1248/bpb.17.467 |
| format | Article |
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The haptens were linked to the carrier protein through hemisuccinate and hemisuccinylglycine bridges at the C-3' and C-3" positions in the digitoxose chain. The antisera were prepared by immunizing rabbits with each digitoxin-BSA conjugate and the properties of the antisera were investigated by RIA with 3H-labeled digitoxin. Among these antisera, the antiserum raised against digitoxin 3'-hemisuccinate-BSA conjugate possessed high specificity for digitoxin, exhibiting only minor cross-reactions with digitoxigenin bisdigitoxoside (4.0%), dihydrodigitoxin (2.8%), digoxin (2.4%), digitoxigenin monodigitoxoside (0.23%) and digitoxigenin (< 0.05%).</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.17.467</identifier><identifier>PMID: 8069249</identifier><language>eng</language><publisher>Tokyo: Maruzen</publisher><subject>Analysis ; Animals ; Biological and medical sciences ; Cattle ; Cross Reactions ; Cross-Linking Reagents ; Digitoxin - chemistry ; Digitoxin - immunology ; General pharmacology ; Haptens ; Hexoses - chemistry ; Immune Sera - immunology ; Medical sciences ; Pharmacology. Drug treatments ; Rabbits ; Radioimmunoassay ; Serum Albumin, Bovine - immunology ; Spectrometry, Mass, Fast Atom Bombardment</subject><ispartof>Biological & pharmaceutical bulletin, 1994, Vol.17 (4), p.467-471</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-39fe79867092d9f74de54b97d81a9e2e44da5e4b02ea59bfd2c6117a722600343</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,4025,27928,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4179041$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8069249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FUJII, Y</creatorcontrib><creatorcontrib>IKEDA, Y</creatorcontrib><creatorcontrib>FUJII, M</creatorcontrib><creatorcontrib>YAMAZAKI, M</creatorcontrib><title>Preparation and antigenic properties of digitoxin-bovine serum albumin conjugates linked at the digitoxose C-3' and C-3 positions</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>In order to obtain specific antisera to digitoxin, four new types of hapten-bovine serum albumin (BSA) conjugates were synthesized from digitoxin. The haptens were linked to the carrier protein through hemisuccinate and hemisuccinylglycine bridges at the C-3' and C-3" positions in the digitoxose chain. The antisera were prepared by immunizing rabbits with each digitoxin-BSA conjugate and the properties of the antisera were investigated by RIA with 3H-labeled digitoxin. Among these antisera, the antiserum raised against digitoxin 3'-hemisuccinate-BSA conjugate possessed high specificity for digitoxin, exhibiting only minor cross-reactions with digitoxigenin bisdigitoxoside (4.0%), dihydrodigitoxin (2.8%), digoxin (2.4%), digitoxigenin monodigitoxoside (0.23%) and digitoxigenin (< 0.05%).</description><subject>Analysis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cross Reactions</subject><subject>Cross-Linking Reagents</subject><subject>Digitoxin - chemistry</subject><subject>Digitoxin - immunology</subject><subject>General pharmacology</subject><subject>Haptens</subject><subject>Hexoses - chemistry</subject><subject>Immune Sera - immunology</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Rabbits</subject><subject>Radioimmunoassay</subject><subject>Serum Albumin, Bovine - immunology</subject><subject>Spectrometry, Mass, Fast Atom Bombardment</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1LxDAQxYMouq5evAs5iILQNUmTpjnK4hcIetBzSdvpGm2TmqSiR_9zo64ehhmY37zHPIQOKFlQxsuzeqwXVC54ITfQjOZcZoJRsYlmRNEyK6god9BuCM-EEElYvo22S1IoxtUMfd57GLXX0TiLtW1TRbMCaxo8ejeCjwYCdh1uzcpE925sVrs3YwEH8NOAdV9Pg7G4cfZ5WumY4N7YF0hCEccn-LtzAfAyy09-PNKARxfMt2nYQ1ud7gPsr_scPV5ePCyvs9u7q5vl-W3WCMpjlqsOpCoLSRRrVSd5C4LXSrYl1QoYcN5qAbwmDLRQddeypqBUaslYQUjO8zk6_tVNf71OEGI1mNBA32sLbgqVLApJSyESePoLNt6F4KGrRm8G7T8qSqrvvKuUd0VllfJO8OFadaoHaP_RdcBpf7Te69DovvPaNib8Y5xKRTjNvwDhnYlU</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>FUJII, Y</creator><creator>IKEDA, Y</creator><creator>FUJII, M</creator><creator>YAMAZAKI, M</creator><general>Maruzen</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>Preparation and antigenic properties of digitoxin-bovine serum albumin conjugates linked at the digitoxose C-3' and C-3 positions</title><author>FUJII, Y ; IKEDA, Y ; FUJII, M ; YAMAZAKI, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-39fe79867092d9f74de54b97d81a9e2e44da5e4b02ea59bfd2c6117a722600343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cross Reactions</topic><topic>Cross-Linking Reagents</topic><topic>Digitoxin - chemistry</topic><topic>Digitoxin - immunology</topic><topic>General pharmacology</topic><topic>Haptens</topic><topic>Hexoses - chemistry</topic><topic>Immune Sera - immunology</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Rabbits</topic><topic>Radioimmunoassay</topic><topic>Serum Albumin, Bovine - immunology</topic><topic>Spectrometry, Mass, Fast Atom Bombardment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FUJII, Y</creatorcontrib><creatorcontrib>IKEDA, Y</creatorcontrib><creatorcontrib>FUJII, M</creatorcontrib><creatorcontrib>YAMAZAKI, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FUJII, Y</au><au>IKEDA, Y</au><au>FUJII, M</au><au>YAMAZAKI, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and antigenic properties of digitoxin-bovine serum albumin conjugates linked at the digitoxose C-3' and C-3 positions</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1994</date><risdate>1994</risdate><volume>17</volume><issue>4</issue><spage>467</spage><epage>471</epage><pages>467-471</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>In order to obtain specific antisera to digitoxin, four new types of hapten-bovine serum albumin (BSA) conjugates were synthesized from digitoxin. The haptens were linked to the carrier protein through hemisuccinate and hemisuccinylglycine bridges at the C-3' and C-3" positions in the digitoxose chain. The antisera were prepared by immunizing rabbits with each digitoxin-BSA conjugate and the properties of the antisera were investigated by RIA with 3H-labeled digitoxin. Among these antisera, the antiserum raised against digitoxin 3'-hemisuccinate-BSA conjugate possessed high specificity for digitoxin, exhibiting only minor cross-reactions with digitoxigenin bisdigitoxoside (4.0%), dihydrodigitoxin (2.8%), digoxin (2.4%), digitoxigenin monodigitoxoside (0.23%) and digitoxigenin (< 0.05%).</abstract><cop>Tokyo</cop><pub>Maruzen</pub><pmid>8069249</pmid><doi>10.1248/bpb.17.467</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0918-6158 |
| ispartof | Biological & pharmaceutical bulletin, 1994, Vol.17 (4), p.467-471 |
| issn | 0918-6158 1347-5215 |
| language | eng |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Biological and medical sciences Cattle Cross Reactions Cross-Linking Reagents Digitoxin - chemistry Digitoxin - immunology General pharmacology Haptens Hexoses - chemistry Immune Sera - immunology Medical sciences Pharmacology. Drug treatments Rabbits Radioimmunoassay Serum Albumin, Bovine - immunology Spectrometry, Mass, Fast Atom Bombardment |
| title | Preparation and antigenic properties of digitoxin-bovine serum albumin conjugates linked at the digitoxose C-3' and C-3 positions |
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