Expression of secreted recombinant human insulin-like growth factor-II (IGF-II) in Chinese hamster ovary cells

Chinese hamster ovary (CHO-KI) cells were cotransfected with a plasmid pcDNAI containing the human preproinsulin-like growth factor II cDNA linked downstream to the human cytomegalovirus promoter and with a plasmid containing the neomycin resistance gene (pMAM-neo). CHO neo + were selected by growth...

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Veröffentlicht in:	Journal of biotechnology 1994-07, Vol.36 (1), p.75-83
Hauptverfasser:	Bekkari, Hicham, Sekkat, Driss, Straczek, Jean, Hess, Ketsia, Belleville-Nabet, Francine, Nabet, Pierre
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Biotechnology Chinese hamster ovary cell CHO Cells - metabolism Cotransfection Cricetinae DNA, Complementary - analysis DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Human insulin-like growth factor II Humans Insulin-Like Growth Factor II - biosynthesis Insulin-Like Growth Factor II - genetics Insulin-Like Growth Factor II - isolation & purification Methods. Procedures. Technologies Mitogenic bioassay Molecular Sequence Data Polymerase chain reaction Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Reversed-phase high-performance liquid chromatography Vectors (cloning, transfer, expression). Insertion sequences and transposons
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