Expression of secreted recombinant human insulin-like growth factor-II (IGF-II) in Chinese hamster ovary cells
Chinese hamster ovary (CHO-KI) cells were cotransfected with a plasmid pcDNAI containing the human preproinsulin-like growth factor II cDNA linked downstream to the human cytomegalovirus promoter and with a plasmid containing the neomycin resistance gene (pMAM-neo). CHO neo + were selected by growth...
Gespeichert in:
| Veröffentlicht in: | Journal of biotechnology 1994-07, Vol.36 (1), p.75-83 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 83 |
|---|---|
| container_issue | 1 |
| container_start_page | 75 |
| container_title | Journal of biotechnology |
| container_volume | 36 |
| creator | Bekkari, Hicham Sekkat, Driss Straczek, Jean Hess, Ketsia Belleville-Nabet, Francine Nabet, Pierre |
| description | Chinese hamster ovary (CHO-KI) cells were cotransfected with a plasmid pcDNAI containing the human preproinsulin-like growth factor II cDNA linked downstream to the human cytomegalovirus promoter and with a plasmid containing the neomycin resistance gene (pMAM-neo). CHO neo
+ were selected by growth in medium supplemented with G418 geneticin. After amplification, the neomycin-resistant clones were screened for IGF-II production. IGF-II produced was identified by dot blot and quantified by ELISA. The clones C24, C40 and C94 secreted IGF-II at about 350–400 ng per 10
6 cells per day. DNA analysis of C24 and C40 CHO cells by PCR demonstrated the presence of the IGF-II construct in the transfected cells, presumably integrated into the chromosomal DNA. IGF-II produced by CHO cells and purified by RP-HPLC was a mitogen for MCF-7 stimulating mitosis 2-fold. |
| doi_str_mv | 10.1016/0168-1656(94)90025-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76663537</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0168165694900256</els_id><sourcerecordid>76663537</sourcerecordid><originalsourceid>FETCH-LOGICAL-c402t-74d87b44d596479a0c14806689361d9fbeb87b5bc1e46d4575fc57b12cc9a9e03</originalsourceid><addsrcrecordid>eNqFkU9v1DAQxS0EKtuFbwCSDwi1h4Cd-E98QUKrtqxUiQucLceZsIbEXjxJC98eL7vaIxxGc3i_Gc28R8grzt5xxtX7Um3FlVRXRlwbxmpZqSdkxVvdVKJVzVOyOiPPySXid8aYMJJfkAutleSKr0i8-bXPgBhSpGmgCD7DDD3N4NPUhejiTHfL5CINEZcxxGoMP4B-y-lx3tHB-TnlarulV9u729KvC0Y3uxABge7chDNkmh5c_k09jCO-IM8GNyK8PPU1-Xp782Xzqbr_fLfdfLyvvGD1XGnRt7oTopdGCW0c81y0TKnWNIr3ZuigK7rsPAeheiG1HLzUHa-9N84Aa9bk7XHvPqefC-Bsp4CHC1yEtKDVSqlGNvq_IFeKa1PcXBNxBH1OiBkGu89hKo9ZzuwhD3sw2x7MtkbYv3nYw9jr0_6lm6A_D50CKPqbk-7Qu3HILvqAZ0zUNeemLtiHIwbFtIcA2aIPED30oSQ12z6Ff9_xBzfXpeQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16617986</pqid></control><display><type>article</type><title>Expression of secreted recombinant human insulin-like growth factor-II (IGF-II) in Chinese hamster ovary cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Bekkari, Hicham ; Sekkat, Driss ; Straczek, Jean ; Hess, Ketsia ; Belleville-Nabet, Francine ; Nabet, Pierre</creator><creatorcontrib>Bekkari, Hicham ; Sekkat, Driss ; Straczek, Jean ; Hess, Ketsia ; Belleville-Nabet, Francine ; Nabet, Pierre</creatorcontrib><description>Chinese hamster ovary (CHO-KI) cells were cotransfected with a plasmid pcDNAI containing the human preproinsulin-like growth factor II cDNA linked downstream to the human cytomegalovirus promoter and with a plasmid containing the neomycin resistance gene (pMAM-neo). CHO neo
+ were selected by growth in medium supplemented with G418 geneticin. After amplification, the neomycin-resistant clones were screened for IGF-II production. IGF-II produced was identified by dot blot and quantified by ELISA. The clones C24, C40 and C94 secreted IGF-II at about 350–400 ng per 10
6 cells per day. DNA analysis of C24 and C40 CHO cells by PCR demonstrated the presence of the IGF-II construct in the transfected cells, presumably integrated into the chromosomal DNA. IGF-II produced by CHO cells and purified by RP-HPLC was a mitogen for MCF-7 stimulating mitosis 2-fold.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/0168-1656(94)90025-6</identifier><identifier>PMID: 7765161</identifier><identifier>CODEN: JBITD4</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Chinese hamster ovary cell ; CHO Cells - metabolism ; Cotransfection ; Cricetinae ; DNA, Complementary - analysis ; DNA, Complementary - genetics ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Human insulin-like growth factor II ; Humans ; Insulin-Like Growth Factor II - biosynthesis ; Insulin-Like Growth Factor II - genetics ; Insulin-Like Growth Factor II - isolation & purification ; Methods. Procedures. Technologies ; Mitogenic bioassay ; Molecular Sequence Data ; Polymerase chain reaction ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - isolation & purification ; Reversed-phase high-performance liquid chromatography ; Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><ispartof>Journal of biotechnology, 1994-07, Vol.36 (1), p.75-83</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c402t-74d87b44d596479a0c14806689361d9fbeb87b5bc1e46d4575fc57b12cc9a9e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-1656(94)90025-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4221192$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7765161$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bekkari, Hicham</creatorcontrib><creatorcontrib>Sekkat, Driss</creatorcontrib><creatorcontrib>Straczek, Jean</creatorcontrib><creatorcontrib>Hess, Ketsia</creatorcontrib><creatorcontrib>Belleville-Nabet, Francine</creatorcontrib><creatorcontrib>Nabet, Pierre</creatorcontrib><title>Expression of secreted recombinant human insulin-like growth factor-II (IGF-II) in Chinese hamster ovary cells</title><title>Journal of biotechnology</title><addtitle>J Biotechnol</addtitle><description>Chinese hamster ovary (CHO-KI) cells were cotransfected with a plasmid pcDNAI containing the human preproinsulin-like growth factor II cDNA linked downstream to the human cytomegalovirus promoter and with a plasmid containing the neomycin resistance gene (pMAM-neo). CHO neo
+ were selected by growth in medium supplemented with G418 geneticin. After amplification, the neomycin-resistant clones were screened for IGF-II production. IGF-II produced was identified by dot blot and quantified by ELISA. The clones C24, C40 and C94 secreted IGF-II at about 350–400 ng per 10
6 cells per day. DNA analysis of C24 and C40 CHO cells by PCR demonstrated the presence of the IGF-II construct in the transfected cells, presumably integrated into the chromosomal DNA. IGF-II produced by CHO cells and purified by RP-HPLC was a mitogen for MCF-7 stimulating mitosis 2-fold.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chinese hamster ovary cell</subject><subject>CHO Cells - metabolism</subject><subject>Cotransfection</subject><subject>Cricetinae</subject><subject>DNA, Complementary - analysis</subject><subject>DNA, Complementary - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Human insulin-like growth factor II</subject><subject>Humans</subject><subject>Insulin-Like Growth Factor II - biosynthesis</subject><subject>Insulin-Like Growth Factor II - genetics</subject><subject>Insulin-Like Growth Factor II - isolation & purification</subject><subject>Methods. Procedures. Technologies</subject><subject>Mitogenic bioassay</subject><subject>Molecular Sequence Data</subject><subject>Polymerase chain reaction</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Reversed-phase high-performance liquid chromatography</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EKtuFbwCSDwi1h4Cd-E98QUKrtqxUiQucLceZsIbEXjxJC98eL7vaIxxGc3i_Gc28R8grzt5xxtX7Um3FlVRXRlwbxmpZqSdkxVvdVKJVzVOyOiPPySXid8aYMJJfkAutleSKr0i8-bXPgBhSpGmgCD7DDD3N4NPUhejiTHfL5CINEZcxxGoMP4B-y-lx3tHB-TnlarulV9u729KvC0Y3uxABge7chDNkmh5c_k09jCO-IM8GNyK8PPU1-Xp782Xzqbr_fLfdfLyvvGD1XGnRt7oTopdGCW0c81y0TKnWNIr3ZuigK7rsPAeheiG1HLzUHa-9N84Aa9bk7XHvPqefC-Bsp4CHC1yEtKDVSqlGNvq_IFeKa1PcXBNxBH1OiBkGu89hKo9ZzuwhD3sw2x7MtkbYv3nYw9jr0_6lm6A_D50CKPqbk-7Qu3HILvqAZ0zUNeemLtiHIwbFtIcA2aIPED30oSQ12z6Ff9_xBzfXpeQ</recordid><startdate>19940729</startdate><enddate>19940729</enddate><creator>Bekkari, Hicham</creator><creator>Sekkat, Driss</creator><creator>Straczek, Jean</creator><creator>Hess, Ketsia</creator><creator>Belleville-Nabet, Francine</creator><creator>Nabet, Pierre</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19940729</creationdate><title>Expression of secreted recombinant human insulin-like growth factor-II (IGF-II) in Chinese hamster ovary cells</title><author>Bekkari, Hicham ; Sekkat, Driss ; Straczek, Jean ; Hess, Ketsia ; Belleville-Nabet, Francine ; Nabet, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-74d87b44d596479a0c14806689361d9fbeb87b5bc1e46d4575fc57b12cc9a9e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chinese hamster ovary cell</topic><topic>CHO Cells - metabolism</topic><topic>Cotransfection</topic><topic>Cricetinae</topic><topic>DNA, Complementary - analysis</topic><topic>DNA, Complementary - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Human insulin-like growth factor II</topic><topic>Humans</topic><topic>Insulin-Like Growth Factor II - biosynthesis</topic><topic>Insulin-Like Growth Factor II - genetics</topic><topic>Insulin-Like Growth Factor II - isolation & purification</topic><topic>Methods. Procedures. Technologies</topic><topic>Mitogenic bioassay</topic><topic>Molecular Sequence Data</topic><topic>Polymerase chain reaction</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Reversed-phase high-performance liquid chromatography</topic><topic>Vectors (cloning, transfer, expression). Insertion sequences and transposons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bekkari, Hicham</creatorcontrib><creatorcontrib>Sekkat, Driss</creatorcontrib><creatorcontrib>Straczek, Jean</creatorcontrib><creatorcontrib>Hess, Ketsia</creatorcontrib><creatorcontrib>Belleville-Nabet, Francine</creatorcontrib><creatorcontrib>Nabet, Pierre</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bekkari, Hicham</au><au>Sekkat, Driss</au><au>Straczek, Jean</au><au>Hess, Ketsia</au><au>Belleville-Nabet, Francine</au><au>Nabet, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of secreted recombinant human insulin-like growth factor-II (IGF-II) in Chinese hamster ovary cells</atitle><jtitle>Journal of biotechnology</jtitle><addtitle>J Biotechnol</addtitle><date>1994-07-29</date><risdate>1994</risdate><volume>36</volume><issue>1</issue><spage>75</spage><epage>83</epage><pages>75-83</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>Chinese hamster ovary (CHO-KI) cells were cotransfected with a plasmid pcDNAI containing the human preproinsulin-like growth factor II cDNA linked downstream to the human cytomegalovirus promoter and with a plasmid containing the neomycin resistance gene (pMAM-neo). CHO neo
+ were selected by growth in medium supplemented with G418 geneticin. After amplification, the neomycin-resistant clones were screened for IGF-II production. IGF-II produced was identified by dot blot and quantified by ELISA. The clones C24, C40 and C94 secreted IGF-II at about 350–400 ng per 10
6 cells per day. DNA analysis of C24 and C40 CHO cells by PCR demonstrated the presence of the IGF-II construct in the transfected cells, presumably integrated into the chromosomal DNA. IGF-II produced by CHO cells and purified by RP-HPLC was a mitogen for MCF-7 stimulating mitosis 2-fold.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>7765161</pmid><doi>10.1016/0168-1656(94)90025-6</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1656 |
| ispartof | Journal of biotechnology, 1994-07, Vol.36 (1), p.75-83 |
| issn | 0168-1656 1873-4863 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76663537 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Base Sequence Biological and medical sciences Biotechnology Chinese hamster ovary cell CHO Cells - metabolism Cotransfection Cricetinae DNA, Complementary - analysis DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Human insulin-like growth factor II Humans Insulin-Like Growth Factor II - biosynthesis Insulin-Like Growth Factor II - genetics Insulin-Like Growth Factor II - isolation & purification Methods. Procedures. Technologies Mitogenic bioassay Molecular Sequence Data Polymerase chain reaction Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Reversed-phase high-performance liquid chromatography Vectors (cloning, transfer, expression). Insertion sequences and transposons |
| title | Expression of secreted recombinant human insulin-like growth factor-II (IGF-II) in Chinese hamster ovary cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T15%3A38%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20secreted%20recombinant%20human%20insulin-like%20growth%20factor-II%20(IGF-II)%20in%20Chinese%20hamster%20ovary%20cells&rft.jtitle=Journal%20of%20biotechnology&rft.au=Bekkari,%20Hicham&rft.date=1994-07-29&rft.volume=36&rft.issue=1&rft.spage=75&rft.epage=83&rft.pages=75-83&rft.issn=0168-1656&rft.eissn=1873-4863&rft.coden=JBITD4&rft_id=info:doi/10.1016/0168-1656(94)90025-6&rft_dat=%3Cproquest_cross%3E76663537%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16617986&rft_id=info:pmid/7765161&rft_els_id=0168165694900256&rfr_iscdi=true |