Intermediates in synaptic vesicle recycling revealed by optical imaging of Drosophila neuromuscular junctions
We show that uptake and release of the styryl dye FM-143 may be used to monitor synaptic vesicle exocytosis and recycling at Drosophila larval neuromuscular junctions. At Drosophila nerve terminals, FM1–43 specifically labels subsynaptic domains enriched in synaptotagmin, in a manner that requires C...
Gespeichert in:
| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 1994-08, Vol.13 (2), p.363-375 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 375 |
|---|---|
| container_issue | 2 |
| container_start_page | 363 |
| container_title | Neuron (Cambridge, Mass.) |
| container_volume | 13 |
| creator | Ramaswami, Mani Krishnan, K.S. Kelly, Regis B. |
| description | We show that uptake and release of the styryl dye FM-143 may be used to monitor synaptic vesicle exocytosis and recycling at Drosophila larval neuromuscular junctions. At Drosophila nerve terminals, FM1–43 specifically labels subsynaptic domains enriched in synaptotagmin, in a manner that requires Ca
2+, membrane depolarization, and
shibire (
shi) function. Endocytosis rates, very low in unstimulated synapses, are induced severalfold by the exocytosis of synaptic vesicles. Using
shi
ts1
mutant synapses to separate synaptic vesicle fusion and recycling temporally, we show that recycling events subsequent to the
shi block do not require extracellular Ca
2+. We suggest that two distinct intermediate stages in vesicle recycling may be trapped and analyzed at Drosophila neuromuscular junctions. |
| doi_str_mv | 10.1016/0896-6273(94)90353-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76661648</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0896627394903530</els_id><sourcerecordid>76661648</sourcerecordid><originalsourceid>FETCH-LOGICAL-c454t-4cdad0e90c9758460f14be7680d6196845a031f62751e006494562be7c5e75d73</originalsourceid><addsrcrecordid>eNqFkUtPHDEQhC2UCDaQf0Akn6LkMNBm_BhfIiHyAAkpFzhbXk8PMfLYiz2z0v57PNkVx-TklurrtqqKkHMGFwyYvIROy0ZeqfaL5l81tKJt4IisGGjVcKb1O7J6Q07Ih1KeARgXmh2T4w4kSKZWZLyLE-YRe28nLNRHWnbRbibv6BaLdwFpRrdzwcenOm3RBuzpekfTwthA_WifFi0N9HtOJW3--GBpxDmncS5uDjbT5zm6yadYzsj7wYaCHw_vKXn8-ePh5ra5__3r7ub6vnFc8Knhrrc9oAanlei4hIHxNSrZQS-Zlh0XFlo2VGOCIYDkmgt5VQknUIletafk8_7uJqeXGctkRl8chmAjprkYJaVkknf_BZkUqoMWKsj3oKsmS8bBbHK1nneGgVnqMEvWZsnaaG7-1mGWtU-H-_O6hvy2dMi_6t_2OtY0th6zKc5jdLWPGvtk-uT__cEryF2apw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16578030</pqid></control><display><type>article</type><title>Intermediates in synaptic vesicle recycling revealed by optical imaging of Drosophila neuromuscular junctions</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Ramaswami, Mani ; Krishnan, K.S. ; Kelly, Regis B.</creator><creatorcontrib>Ramaswami, Mani ; Krishnan, K.S. ; Kelly, Regis B.</creatorcontrib><description>We show that uptake and release of the styryl dye FM-143 may be used to monitor synaptic vesicle exocytosis and recycling at Drosophila larval neuromuscular junctions. At Drosophila nerve terminals, FM1–43 specifically labels subsynaptic domains enriched in synaptotagmin, in a manner that requires Ca
2+, membrane depolarization, and
shibire (
shi) function. Endocytosis rates, very low in unstimulated synapses, are induced severalfold by the exocytosis of synaptic vesicles. Using
shi
ts1
mutant synapses to separate synaptic vesicle fusion and recycling temporally, we show that recycling events subsequent to the
shi block do not require extracellular Ca
2+. We suggest that two distinct intermediate stages in vesicle recycling may be trapped and analyzed at Drosophila neuromuscular junctions.</description><identifier>ISSN: 0896-6273</identifier><identifier>EISSN: 1097-4199</identifier><identifier>DOI: 10.1016/0896-6273(94)90353-0</identifier><identifier>PMID: 8060617</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Calcium - physiology ; Calcium-Binding Proteins ; Drosophila ; Drosophila melanogaster ; Drosophila Proteins ; Dynamins ; Endocytosis ; Exocytosis ; GTP Phosphohydrolases - physiology ; Membrane Fusion ; Membrane Glycoproteins - metabolism ; Membrane Potentials ; Nerve Tissue Proteins - metabolism ; Neuromuscular Junction - physiology ; Neuromuscular Junction - ultrastructure ; Potassium - physiology ; Spider Venoms - pharmacology ; Synaptic Transmission ; Synaptic Vesicles - physiology ; Synaptotagmins</subject><ispartof>Neuron (Cambridge, Mass.), 1994-08, Vol.13 (2), p.363-375</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-4cdad0e90c9758460f14be7680d6196845a031f62751e006494562be7c5e75d73</citedby><cites>FETCH-LOGICAL-c454t-4cdad0e90c9758460f14be7680d6196845a031f62751e006494562be7c5e75d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0896-6273(94)90353-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8060617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramaswami, Mani</creatorcontrib><creatorcontrib>Krishnan, K.S.</creatorcontrib><creatorcontrib>Kelly, Regis B.</creatorcontrib><title>Intermediates in synaptic vesicle recycling revealed by optical imaging of Drosophila neuromuscular junctions</title><title>Neuron (Cambridge, Mass.)</title><addtitle>Neuron</addtitle><description>We show that uptake and release of the styryl dye FM-143 may be used to monitor synaptic vesicle exocytosis and recycling at Drosophila larval neuromuscular junctions. At Drosophila nerve terminals, FM1–43 specifically labels subsynaptic domains enriched in synaptotagmin, in a manner that requires Ca
2+, membrane depolarization, and
shibire (
shi) function. Endocytosis rates, very low in unstimulated synapses, are induced severalfold by the exocytosis of synaptic vesicles. Using
shi
ts1
mutant synapses to separate synaptic vesicle fusion and recycling temporally, we show that recycling events subsequent to the
shi block do not require extracellular Ca
2+. We suggest that two distinct intermediate stages in vesicle recycling may be trapped and analyzed at Drosophila neuromuscular junctions.</description><subject>Animals</subject><subject>Calcium - physiology</subject><subject>Calcium-Binding Proteins</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Drosophila Proteins</subject><subject>Dynamins</subject><subject>Endocytosis</subject><subject>Exocytosis</subject><subject>GTP Phosphohydrolases - physiology</subject><subject>Membrane Fusion</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Membrane Potentials</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neuromuscular Junction - physiology</subject><subject>Neuromuscular Junction - ultrastructure</subject><subject>Potassium - physiology</subject><subject>Spider Venoms - pharmacology</subject><subject>Synaptic Transmission</subject><subject>Synaptic Vesicles - physiology</subject><subject>Synaptotagmins</subject><issn>0896-6273</issn><issn>1097-4199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtPHDEQhC2UCDaQf0Akn6LkMNBm_BhfIiHyAAkpFzhbXk8PMfLYiz2z0v57PNkVx-TklurrtqqKkHMGFwyYvIROy0ZeqfaL5l81tKJt4IisGGjVcKb1O7J6Q07Ih1KeARgXmh2T4w4kSKZWZLyLE-YRe28nLNRHWnbRbibv6BaLdwFpRrdzwcenOm3RBuzpekfTwthA_WifFi0N9HtOJW3--GBpxDmncS5uDjbT5zm6yadYzsj7wYaCHw_vKXn8-ePh5ra5__3r7ub6vnFc8Knhrrc9oAanlei4hIHxNSrZQS-Zlh0XFlo2VGOCIYDkmgt5VQknUIletafk8_7uJqeXGctkRl8chmAjprkYJaVkknf_BZkUqoMWKsj3oKsmS8bBbHK1nneGgVnqMEvWZsnaaG7-1mGWtU-H-_O6hvy2dMi_6t_2OtY0th6zKc5jdLWPGvtk-uT__cEryF2apw</recordid><startdate>19940801</startdate><enddate>19940801</enddate><creator>Ramaswami, Mani</creator><creator>Krishnan, K.S.</creator><creator>Kelly, Regis B.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19940801</creationdate><title>Intermediates in synaptic vesicle recycling revealed by optical imaging of Drosophila neuromuscular junctions</title><author>Ramaswami, Mani ; Krishnan, K.S. ; Kelly, Regis B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-4cdad0e90c9758460f14be7680d6196845a031f62751e006494562be7c5e75d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Calcium - physiology</topic><topic>Calcium-Binding Proteins</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Drosophila Proteins</topic><topic>Dynamins</topic><topic>Endocytosis</topic><topic>Exocytosis</topic><topic>GTP Phosphohydrolases - physiology</topic><topic>Membrane Fusion</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Membrane Potentials</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neuromuscular Junction - physiology</topic><topic>Neuromuscular Junction - ultrastructure</topic><topic>Potassium - physiology</topic><topic>Spider Venoms - pharmacology</topic><topic>Synaptic Transmission</topic><topic>Synaptic Vesicles - physiology</topic><topic>Synaptotagmins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramaswami, Mani</creatorcontrib><creatorcontrib>Krishnan, K.S.</creatorcontrib><creatorcontrib>Kelly, Regis B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuron (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramaswami, Mani</au><au>Krishnan, K.S.</au><au>Kelly, Regis B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intermediates in synaptic vesicle recycling revealed by optical imaging of Drosophila neuromuscular junctions</atitle><jtitle>Neuron (Cambridge, Mass.)</jtitle><addtitle>Neuron</addtitle><date>1994-08-01</date><risdate>1994</risdate><volume>13</volume><issue>2</issue><spage>363</spage><epage>375</epage><pages>363-375</pages><issn>0896-6273</issn><eissn>1097-4199</eissn><abstract>We show that uptake and release of the styryl dye FM-143 may be used to monitor synaptic vesicle exocytosis and recycling at Drosophila larval neuromuscular junctions. At Drosophila nerve terminals, FM1–43 specifically labels subsynaptic domains enriched in synaptotagmin, in a manner that requires Ca
2+, membrane depolarization, and
shibire (
shi) function. Endocytosis rates, very low in unstimulated synapses, are induced severalfold by the exocytosis of synaptic vesicles. Using
shi
ts1
mutant synapses to separate synaptic vesicle fusion and recycling temporally, we show that recycling events subsequent to the
shi block do not require extracellular Ca
2+. We suggest that two distinct intermediate stages in vesicle recycling may be trapped and analyzed at Drosophila neuromuscular junctions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8060617</pmid><doi>10.1016/0896-6273(94)90353-0</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0896-6273 |
| ispartof | Neuron (Cambridge, Mass.), 1994-08, Vol.13 (2), p.363-375 |
| issn | 0896-6273 1097-4199 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76661648 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Calcium - physiology Calcium-Binding Proteins Drosophila Drosophila melanogaster Drosophila Proteins Dynamins Endocytosis Exocytosis GTP Phosphohydrolases - physiology Membrane Fusion Membrane Glycoproteins - metabolism Membrane Potentials Nerve Tissue Proteins - metabolism Neuromuscular Junction - physiology Neuromuscular Junction - ultrastructure Potassium - physiology Spider Venoms - pharmacology Synaptic Transmission Synaptic Vesicles - physiology Synaptotagmins |
| title | Intermediates in synaptic vesicle recycling revealed by optical imaging of Drosophila neuromuscular junctions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T13%3A19%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Intermediates%20in%20synaptic%20vesicle%20recycling%20revealed%20by%20optical%20imaging%20of%20Drosophila%20neuromuscular%20junctions&rft.jtitle=Neuron%20(Cambridge,%20Mass.)&rft.au=Ramaswami,%20Mani&rft.date=1994-08-01&rft.volume=13&rft.issue=2&rft.spage=363&rft.epage=375&rft.pages=363-375&rft.issn=0896-6273&rft.eissn=1097-4199&rft_id=info:doi/10.1016/0896-6273(94)90353-0&rft_dat=%3Cproquest_cross%3E76661648%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16578030&rft_id=info:pmid/8060617&rft_els_id=0896627394903530&rfr_iscdi=true |