A Microtiter Plate Assay for Factor XIII A-Chain-Fibrin Interactions

Factor XIII A-chain-fibrin interactions regulate factor XIIIa formation and fibrin cross-linking. A microtiter plate assay was developed for studying these interactions. Microtiter plate wells were coated with fibrinogen and converted to fibrin by thrombin. After blocking the wells with bovine serum...

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Veröffentlicht in:	Analytical biochemistry 1994-05, Vol.219 (1), p.43-48
Hauptverfasser:	Achyuthan, K.E., Santiago, M.A., Greenberg, C.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Boron Compounds - pharmacology Electrophoresis, Polyacrylamide Gel - methods Factor XIII - analysis Factor XIII - isolation & purification Factor XIII - metabolism Fibrin - analysis Fibrin - isolation & purification Fibrin - metabolism Fibrinogen - metabolism Fundamental and applied biological sciences. Psychology Goats - immunology Humans Immunoblotting - methods Kinetics Luminescent Measurements Microchemistry Molecular Weight Oligopeptides - pharmacology Protein Binding Sensitivity and Specificity Serum Albumin, Bovine Sodium Chloride - pharmacology Thrombin - antagonists & inhibitors Thrombin - metabolism
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