IR and Raman spectroscopic studies of the interaction of trehalose with hen egg white lysozyme
Infrared and Fourier transform Raman spectra are reported for dried mixtures of trehalose and lysozyme. The Raman spectra show effects on the protein amide I band and some sugar bands that are not present when the components are dried separately. Comparison of ir spectra with those published previou...
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| Veröffentlicht in: | Biopolymers 1994-07, Vol.34 (7), p.957-961 |
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| container_end_page | 961 |
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| container_title | Biopolymers |
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| creator | Belton, P. S. Gil, A. M. |
| description | Infrared and Fourier transform Raman spectra are reported for dried mixtures of trehalose and lysozyme. The Raman spectra show effects on the protein amide I band and some sugar bands that are not present when the components are dried separately. Comparison of ir spectra with those published previously show significant differences. It is concluded that these arise because of differences in the extent of drying of the moisture, and that, contrary to some claims, vibrational spectroscopy does not so far show any clear evidence of specific trehalose/protein interactions and that results may be interpreted in terms of entrapment of water within the mixture. © 1994 John Wiley & Sons, Inc. |
| doi_str_mv | 10.1002/bip.360340713 |
| format | Article |
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S. ; Gil, A. M.</creator><creatorcontrib>Belton, P. S. ; Gil, A. M.</creatorcontrib><description>Infrared and Fourier transform Raman spectra are reported for dried mixtures of trehalose and lysozyme. The Raman spectra show effects on the protein amide I band and some sugar bands that are not present when the components are dried separately. Comparison of ir spectra with those published previously show significant differences. It is concluded that these arise because of differences in the extent of drying of the moisture, and that, contrary to some claims, vibrational spectroscopy does not so far show any clear evidence of specific trehalose/protein interactions and that results may be interpreted in terms of entrapment of water within the mixture. © 1994 John Wiley & Sons, Inc.</description><identifier>ISSN: 0006-3525</identifier><identifier>EISSN: 1097-0282</identifier><identifier>DOI: 10.1002/bip.360340713</identifier><identifier>PMID: 8054473</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Chickens ; Egg White - analysis ; Female ; Lysosomes - chemistry ; Spectrophotometry, Infrared ; Spectrum Analysis, Raman ; Trehalose - chemistry</subject><ispartof>Biopolymers, 1994-07, Vol.34 (7), p.957-961</ispartof><rights>Copyright © 1994 John Wiley & Sons, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3743-e096d535f3bc53b897c85acf091b565a9f1c8f489a15efb391c148ac8467042e3</citedby><cites>FETCH-LOGICAL-c3743-e096d535f3bc53b897c85acf091b565a9f1c8f489a15efb391c148ac8467042e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbip.360340713$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbip.360340713$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8054473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Belton, P. 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It is concluded that these arise because of differences in the extent of drying of the moisture, and that, contrary to some claims, vibrational spectroscopy does not so far show any clear evidence of specific trehalose/protein interactions and that results may be interpreted in terms of entrapment of water within the mixture. © 1994 John Wiley & Sons, Inc.</description><subject>Animals</subject><subject>Chickens</subject><subject>Egg White - analysis</subject><subject>Female</subject><subject>Lysosomes - chemistry</subject><subject>Spectrophotometry, Infrared</subject><subject>Spectrum Analysis, Raman</subject><subject>Trehalose - chemistry</subject><issn>0006-3525</issn><issn>1097-0282</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PGzEQhq2qiAbokSOST70tjNf22ntsEUmDEFQU6K2W15ll3e5X145C-PUsJIp64jTSzDOPZl5CjhmcMoD0rPD9Kc-AC1CMfyATBrlKINXpRzIBgCzhMpWfyEEIfwCE4Az2yb4GKYTiE_J7fkttu6C3trEtDT26OHTBdb13NMTlwmOgXUljhdS3EQfrou_at9aAla27gHTlY0UrbCk-PtJV5SPSeh2653WDR2SvtHXAz9t6SO6nF3fn35Orm9n8_OtV4rgSPEHIs4XksuSFk7zQuXJaWldCzgqZSZuXzOlS6NwyiWXBc-aY0NZpkSkQKfJD8mXj7Yfu3xJDNI0PDuvattgtg1FZJrQUMILJBnTjm2HA0vSDb-ywNgzMa55mzNPs8hz5k614WTS42NHbAMe52sxXvsb1-zLzbf7jf_P2Eh8iPu027fDXZIoraX5dz8wlU_AgplPzk78AXX6QLA</recordid><startdate>199407</startdate><enddate>199407</enddate><creator>Belton, P. 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It is concluded that these arise because of differences in the extent of drying of the moisture, and that, contrary to some claims, vibrational spectroscopy does not so far show any clear evidence of specific trehalose/protein interactions and that results may be interpreted in terms of entrapment of water within the mixture. © 1994 John Wiley & Sons, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8054473</pmid><doi>10.1002/bip.360340713</doi><tpages>5</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Animals Chickens Egg White - analysis Female Lysosomes - chemistry Spectrophotometry, Infrared Spectrum Analysis, Raman Trehalose - chemistry |
| title | IR and Raman spectroscopic studies of the interaction of trehalose with hen egg white lysozyme |
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