Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel
(125I)-ovine prolactin (oPRL) binding was found in several brain areas of the toad, Bufo arenarum Hensel. The olfactory bulb, cerebral hemispheres, and both dorsal and ventral mesencephalic regions showed saturable, high affinity, (125I)-oPRL binding, ranging between 5.6 to 29.9 fmol/mg protein, whi...
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| Veröffentlicht in: | Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology Biochemical, systemic, and environmental physiology, 1985-01, Vol.155 (5), p.611-614 |
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| container_end_page | 614 |
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| container_issue | 5 |
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| container_title | Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology |
| container_volume | 155 |
| creator | LUTHY, I. A SEGURA, E. T LUTHY, V. I CHARREAU, E. H CALANDRA, R. S |
| description | (125I)-ovine prolactin (oPRL) binding was found in several brain areas of the toad, Bufo arenarum Hensel. The olfactory bulb, cerebral hemispheres, and both dorsal and ventral mesencephalic regions showed saturable, high affinity, (125I)-oPRL binding, ranging between 5.6 to 29.9 fmol/mg protein, while the association constant (Ka) by Scatchard analysis was between 4.0 to 8.7 x 10(9) M-1. This binding was compared with the Scatchard plot of the kidney, which has been already described by other groups, and gave 41.7 fmol/mg protein and Ka 2.5 x 10(9) M-1. Liver showed no binding and in the cerebral hemispheres (125I)-oPRL was not displaced by non-lactogenic hormones, indicating that binding was hormone and tissue specific. |
| doi_str_mv | 10.1007/BF00694452 |
| format | Article |
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A ; SEGURA, E. T ; LUTHY, V. I ; CHARREAU, E. H ; CALANDRA, R. S</creator><creatorcontrib>LUTHY, I. A ; SEGURA, E. T ; LUTHY, V. I ; CHARREAU, E. H ; CALANDRA, R. S</creatorcontrib><description>(125I)-ovine prolactin (oPRL) binding was found in several brain areas of the toad, Bufo arenarum Hensel. The olfactory bulb, cerebral hemispheres, and both dorsal and ventral mesencephalic regions showed saturable, high affinity, (125I)-oPRL binding, ranging between 5.6 to 29.9 fmol/mg protein, while the association constant (Ka) by Scatchard analysis was between 4.0 to 8.7 x 10(9) M-1. This binding was compared with the Scatchard plot of the kidney, which has been already described by other groups, and gave 41.7 fmol/mg protein and Ka 2.5 x 10(9) M-1. Liver showed no binding and in the cerebral hemispheres (125I)-oPRL was not displaced by non-lactogenic hormones, indicating that binding was hormone and tissue specific.</description><identifier>ISSN: 0174-1578</identifier><identifier>EISSN: 1432-136X</identifier><identifier>DOI: 10.1007/BF00694452</identifier><identifier>PMID: 3016041</identifier><identifier>CODEN: JPBPDL</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Binding, Competitive ; Biological and medical sciences ; brain ; Brain - metabolism ; Bufo arenarum ; Fundamental and applied biological sciences. Psychology ; Hormones and neuropeptides. Regulation ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; Kidney - metabolism ; Kinetics ; Male ; prolactin ; Prolactin - metabolism ; Receptors, Cell Surface - metabolism ; Receptors, Prolactin ; Sheep ; Tissue Distribution ; Vertebrates: endocrinology</subject><ispartof>Journal of comparative physiology. 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T</creatorcontrib><creatorcontrib>LUTHY, V. I</creatorcontrib><creatorcontrib>CHARREAU, E. H</creatorcontrib><creatorcontrib>CALANDRA, R. S</creatorcontrib><title>Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel</title><title>Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology</title><addtitle>J Comp Physiol B</addtitle><description>(125I)-ovine prolactin (oPRL) binding was found in several brain areas of the toad, Bufo arenarum Hensel. The olfactory bulb, cerebral hemispheres, and both dorsal and ventral mesencephalic regions showed saturable, high affinity, (125I)-oPRL binding, ranging between 5.6 to 29.9 fmol/mg protein, while the association constant (Ka) by Scatchard analysis was between 4.0 to 8.7 x 10(9) M-1. This binding was compared with the Scatchard plot of the kidney, which has been already described by other groups, and gave 41.7 fmol/mg protein and Ka 2.5 x 10(9) M-1. Liver showed no binding and in the cerebral hemispheres (125I)-oPRL was not displaced by non-lactogenic hormones, indicating that binding was hormone and tissue specific.</description><subject>Animals</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>brain</subject><subject>Brain - metabolism</subject><subject>Bufo arenarum</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Kidney - metabolism</subject><subject>Kinetics</subject><subject>Male</subject><subject>prolactin</subject><subject>Prolactin - metabolism</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Receptors, Prolactin</subject><subject>Sheep</subject><subject>Tissue Distribution</subject><subject>Vertebrates: endocrinology</subject><issn>0174-1578</issn><issn>1432-136X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAUhS0EgvJY2JE8IAZEwK84zkgroEhIIAQSW3RrX0MgdcBOhv57Aq3KyHSP7vfpDIeQQ87OOWPFxfiaMV0qlYsNMuJKioxL_bJJRowXKuN5YXbIbkrvjDHFjdom25JxPeQReXyIbQO2qwOd1cHV4ZWmusNEh0f3hnQWYUgQHP2oXcBFoq3_BV0L7oyOe99SiBgg9nM6xZCw2SdbHpqEB6u7R56vr54m0-zu_uZ2cnmXWalEl7ncGq4ls8wrp1HnzgiBhZeunAlECwilRQOgrBBeMGsNOmDeOy21KAu5R06WvZ-x_eoxddW8ThabBgK2faoKrZVRvPxX5ErkujB8EE-Xoo1tShF99RnrOcRFxVn1s3T1t_QgH61a-9kc3VpdTTvw4xWHZKHxEYKt01ozmuWm5PIbVyGE4g</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>LUTHY, I. A</creator><creator>SEGURA, E. T</creator><creator>LUTHY, V. I</creator><creator>CHARREAU, E. H</creator><creator>CALANDRA, R. S</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SQ</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19850101</creationdate><title>Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel</title><author>LUTHY, I. A ; SEGURA, E. T ; LUTHY, V. I ; CHARREAU, E. H ; CALANDRA, R. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-d5c81630c0f4d6e65d822e7f3d9b2eecaea9ce8aa4c22f20cc8eda0ffd6362973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>brain</topic><topic>Brain - metabolism</topic><topic>Bufo arenarum</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Kidney - metabolism</topic><topic>Kinetics</topic><topic>Male</topic><topic>prolactin</topic><topic>Prolactin - metabolism</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Receptors, Prolactin</topic><topic>Sheep</topic><topic>Tissue Distribution</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LUTHY, I. A</creatorcontrib><creatorcontrib>SEGURA, E. T</creatorcontrib><creatorcontrib>LUTHY, V. I</creatorcontrib><creatorcontrib>CHARREAU, E. H</creatorcontrib><creatorcontrib>CALANDRA, R. 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S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel</atitle><jtitle>Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology</jtitle><addtitle>J Comp Physiol B</addtitle><date>1985-01-01</date><risdate>1985</risdate><volume>155</volume><issue>5</issue><spage>611</spage><epage>614</epage><pages>611-614</pages><issn>0174-1578</issn><eissn>1432-136X</eissn><coden>JPBPDL</coden><abstract>(125I)-ovine prolactin (oPRL) binding was found in several brain areas of the toad, Bufo arenarum Hensel. The olfactory bulb, cerebral hemispheres, and both dorsal and ventral mesencephalic regions showed saturable, high affinity, (125I)-oPRL binding, ranging between 5.6 to 29.9 fmol/mg protein, while the association constant (Ka) by Scatchard analysis was between 4.0 to 8.7 x 10(9) M-1. This binding was compared with the Scatchard plot of the kidney, which has been already described by other groups, and gave 41.7 fmol/mg protein and Ka 2.5 x 10(9) M-1. Liver showed no binding and in the cerebral hemispheres (125I)-oPRL was not displaced by non-lactogenic hormones, indicating that binding was hormone and tissue specific.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>3016041</pmid><doi>10.1007/BF00694452</doi><tpages>4</tpages></addata></record> |
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| issn | 0174-1578 1432-136X |
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| subjects | Animals Binding, Competitive Biological and medical sciences brain Brain - metabolism Bufo arenarum Fundamental and applied biological sciences. Psychology Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis Kidney - metabolism Kinetics Male prolactin Prolactin - metabolism Receptors, Cell Surface - metabolism Receptors, Prolactin Sheep Tissue Distribution Vertebrates: endocrinology |
| title | Prolactin binding sites in the brain and kidneys of the toad, Bufo arenarum Hensel |
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