Chromatographic methods for quantitative analysis of native, denatured, and aggregated basic fibroblast growth factor in solution formulations

High-performance liquid chromatography (HPLC) methods were developed for evaluating stability of human recombinant basic fibroblast growth factor (bFGF) against denaturation and aggregation in solution formulations. Reversed-phase chromatography (RP-HPLC)-insensitive to bFGF tertiary structure--was...

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Veröffentlicht in:	Pharmaceutical research 1994-04, Vol.11 (4), p.485-490
Hauptverfasser:	SLUZKY, V, SHAKROKH, Z, STRATTON, P, EBERLEIN, G, WANG, Y. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Chemical Phenomena Chemistry, Physical Chromatography, Affinity Chromatography, Gel Chromatography, High Pressure Liquid Circular Dichroism Fibroblast Growth Factor 2 - administration & dosage Fibroblast Growth Factor 2 - analysis General pharmacology Heparin - chemistry Humans Indicators and Reagents Medical sciences Molecular Weight Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Protein Denaturation Protein Structure, Tertiary Recombinant Proteins - administration & dosage Recombinant Proteins - analysis Solutions Spectrometry, Fluorescence Spectrophotometry, Ultraviolet
Online-Zugang:	Volltext
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