Nitric oxide synthase: MRNA expression of different isoforms in human monocytes/macrophages

To detect mRNA expression of nitric oxide synthase (NOS) isoforms in human monocytes/macrophages reverse transcription polymerase chain reaction (RT‐PCR) was used. mRNA was isolated from stimulated or unstimulated monocytes/macrophages and RT‐PCR was performed using oligonucleotide primers derived f...

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