Generation and characterization of novel antibodies highly selective for phosphorylated linker histone H1 in Tetrahymena and HeLa cells

Phosphorylated forms of Tetrahymena macronuclear histone H1 were separated from each other and from dephosphorylated H1 by cation-exchange HPLC. A homogeneous fraction of hyperphosphorylated macronuclear H1 was then used to generate novel polyclonal antibodies highly selective for phosphorylated H1...

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Veröffentlicht in:	Chromosoma 1994-04, Vol.103 (2), p.111-121
Hauptverfasser:	LU, M. J, DADD, C. A, MIZZEN, C. A, PERRY, C. A, MCLACHLAN, D. R, ANNUNZIATO, A. T, ALLIS, C. D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Protozoan - immunology Antibody Specificity Biological and medical sciences Cell Cycle Chromatography, High Pressure Liquid Cytogenetics Epitopes - analysis Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution HeLa Cells Histones - analysis Histones - immunology Histones - isolation & purification Histones - metabolism Human Humans Immunization Phosphorylation Rabbits Tetrahymena - chemistry Tetrahymena - immunology Tetrahymena pigmentosa
Online-Zugang:	Volltext
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