Secretion of Sucrase by Leishmania donovani
Leishmania donovani promastigotes were collected, washed, resuspended in buffer, and assayed for sucrase activity. No activity was observed in the intact washed cells, but activity was measurable when the cells were permeabilized with Triton X‐100. Intracellular sucrase activity was highest in proma...
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| Veröffentlicht in: | The Journal of eukaryotic microbiology 1994-05, Vol.41 (3), p.228-231 |
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| container_title | The Journal of eukaryotic microbiology |
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| creator | BLUM, J. JOSEPH OPPERDOES, FRED R. |
| description | Leishmania donovani promastigotes were collected, washed, resuspended in buffer, and assayed for sucrase activity. No activity was observed in the intact washed cells, but activity was measurable when the cells were permeabilized with Triton X‐100. Intracellular sucrase activity was highest in promastigotes grown at pH 7.4, somewhat lower in promastigotes grown at pH 5.5, and significantly lower in “amastigotes” grown at pH 5.5. No trehalase, lactase, or maltase activities were observed. Assay of the medium in which the cells had grown showed that most the sucrase activity was extracellular, i.e. was secreted into the medium during growth. |
| doi_str_mv | 10.1111/j.1550-7408.1994.tb01502.x |
| format | Article |
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Assay of the medium in which the cells had grown showed that most the sucrase activity was extracellular, i.e. was secreted into the medium during growth.</description><identifier>ISSN: 1066-5234</identifier><identifier>EISSN: 1550-7408</identifier><identifier>DOI: 10.1111/j.1550-7408.1994.tb01502.x</identifier><identifier>PMID: 8049686</identifier><identifier>CODEN: JEMIED</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Biological and medical sciences ; Culture Media ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Invertebrates ; Leishmania donovani ; Leishmania donovani - enzymology ; Life cycle. Host-agent relationship. 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JOSEPH</creatorcontrib><creatorcontrib>OPPERDOES, FRED R.</creatorcontrib><title>Secretion of Sucrase by Leishmania donovani</title><title>The Journal of eukaryotic microbiology</title><addtitle>J Eukaryot Microbiol</addtitle><description>Leishmania donovani promastigotes were collected, washed, resuspended in buffer, and assayed for sucrase activity. No activity was observed in the intact washed cells, but activity was measurable when the cells were permeabilized with Triton X‐100. Intracellular sucrase activity was highest in promastigotes grown at pH 7.4, somewhat lower in promastigotes grown at pH 5.5, and significantly lower in “amastigotes” grown at pH 5.5. No trehalase, lactase, or maltase activities were observed. Assay of the medium in which the cells had grown showed that most the sucrase activity was extracellular, i.e. was secreted into the medium during growth.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Culture Media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Invertebrates</subject><subject>Leishmania donovani</subject><subject>Leishmania donovani - enzymology</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Octoxynol</subject><subject>Parasite cultures</subject><subject>Protozoa</subject><subject>Sucrase - secretion</subject><issn>1066-5234</issn><issn>1550-7408</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkFtLxDAQhYMo3n-CUER8kdYkzeTig6CLusqiiIqPIU1T7NptNdnV3X9v65Z9FfMyE86ZM8OH0CHBCWnf6TghADgWDMuEKMWSaYYJYJrM19D2Slpve8x5DDRlW2gnhDHGhFNCNtGmxExxybfRyZOz3k3Lpo6aInqaWW-Ci7JFNHJleJuYujRR3tTNV9vtoY3CVMHt93UXvVxfPQ-G8ejh5nZwMYot40LGFAOWaS4wYzID4Hn7FYZIRpiVloESQDOpCsgVVgzAFgYks1ymvDCuLbvoeJn74ZvPmQtTPSmDdVVlatfMghacpxIT-qeRcBBYAbTGs6XR-iYE7wr94cuJ8QtNsO6Q6rHuuOmOm-6Q6h6pnrfDB_2WWTZx-Wq0Z9jqR71ugjVV4U1ty7CyMUok-b3hfGn7Liu3-McB-u7qhdIOS7wMKMPUzVcBxr9rLlIB-vX-Rg-H6pI83j_rQfoDrrWe2w</recordid><startdate>199405</startdate><enddate>199405</enddate><creator>BLUM, J. JOSEPH</creator><creator>OPPERDOES, FRED R.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>199405</creationdate><title>Secretion of Sucrase by Leishmania donovani</title><author>BLUM, J. JOSEPH ; OPPERDOES, FRED R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4678-205083d70448b556d5087a18414c8c459752b89f5d909455cfa584c6836fae683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Culture Media</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Invertebrates</topic><topic>Leishmania donovani</topic><topic>Leishmania donovani - enzymology</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Octoxynol</topic><topic>Parasite cultures</topic><topic>Protozoa</topic><topic>Sucrase - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BLUM, J. JOSEPH</creatorcontrib><creatorcontrib>OPPERDOES, FRED R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of eukaryotic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BLUM, J. JOSEPH</au><au>OPPERDOES, FRED R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Secretion of Sucrase by Leishmania donovani</atitle><jtitle>The Journal of eukaryotic microbiology</jtitle><addtitle>J Eukaryot Microbiol</addtitle><date>1994-05</date><risdate>1994</risdate><volume>41</volume><issue>3</issue><spage>228</spage><epage>231</epage><pages>228-231</pages><issn>1066-5234</issn><eissn>1550-7408</eissn><coden>JEMIED</coden><abstract>Leishmania donovani promastigotes were collected, washed, resuspended in buffer, and assayed for sucrase activity. No activity was observed in the intact washed cells, but activity was measurable when the cells were permeabilized with Triton X‐100. Intracellular sucrase activity was highest in promastigotes grown at pH 7.4, somewhat lower in promastigotes grown at pH 5.5, and significantly lower in “amastigotes” grown at pH 5.5. No trehalase, lactase, or maltase activities were observed. Assay of the medium in which the cells had grown showed that most the sucrase activity was extracellular, i.e. was secreted into the medium during growth.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8049686</pmid><doi>10.1111/j.1550-7408.1994.tb01502.x</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 1066-5234 |
| ispartof | The Journal of eukaryotic microbiology, 1994-05, Vol.41 (3), p.228-231 |
| issn | 1066-5234 1550-7408 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biological and medical sciences Culture Media Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Invertebrates Leishmania donovani Leishmania donovani - enzymology Life cycle. Host-agent relationship. Pathogenesis Octoxynol Parasite cultures Protozoa Sucrase - secretion |
| title | Secretion of Sucrase by Leishmania donovani |
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