The herpes simplex virus type 1 UL37 gene product is a component of virus particles
1 MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, U.K. and 2 International Agricultural College Larenstein, Marijkeweg 5, 6709 PE Wageningen, The Netherlands The herpes simplex virus type 1 UL37 gene encodes a protein with an M r of 120K that is produced at late times after...
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| Veröffentlicht in: | Journal of general virology 1994-08, Vol.75 (8), p.2047-2052 |
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| container_title | Journal of general virology |
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| creator | McLauchlan, John Liefkens, Karin Stow, Nigel D |
| description | 1 MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, U.K.
and 2 International Agricultural College Larenstein, Marijkeweg 5, 6709 PE Wageningen, The Netherlands
The herpes simplex virus type 1 UL37 gene encodes a protein with an M r of 120K that is produced at late times after infection. To study the properties of this protein we have linked a 10 amino acid epitope derived from a human cytomegalovirus protein to the UL37 polypeptide coding sequences by inserting an oligonucleotide at a Spe I site that is unique in the virus genome and lies close to the 3' end of the open reading frame. From studies on the resultant virus recombinant using a monoclonal antibody that recognizes the inserted epitope we find that, contrary to a previous report, the UL37 protein is a structural component of both virions and L particles and is present in the tegument of virus particles. Indirect immunofluorescence analysis revealed that the protein is distributed throughout infected cells but is more abundant in the cytoplasm than the nucleus.
Received 5 January 1994;
accepted 17 February 1994. |
| doi_str_mv | 10.1099/0022-1317-75-8-2047 |
| format | Article |
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and 2 International Agricultural College Larenstein, Marijkeweg 5, 6709 PE Wageningen, The Netherlands
The herpes simplex virus type 1 UL37 gene encodes a protein with an M r of 120K that is produced at late times after infection. To study the properties of this protein we have linked a 10 amino acid epitope derived from a human cytomegalovirus protein to the UL37 polypeptide coding sequences by inserting an oligonucleotide at a Spe I site that is unique in the virus genome and lies close to the 3' end of the open reading frame. From studies on the resultant virus recombinant using a monoclonal antibody that recognizes the inserted epitope we find that, contrary to a previous report, the UL37 protein is a structural component of both virions and L particles and is present in the tegument of virus particles. Indirect immunofluorescence analysis revealed that the protein is distributed throughout infected cells but is more abundant in the cytoplasm than the nucleus.
Received 5 January 1994;
accepted 17 February 1994.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-75-8-2047</identifier><identifier>PMID: 8046407</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Cell Compartmentation ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Genetics ; herpes simplex virus 1 ; Herpesvirus 1, Human - chemistry ; Microbiology ; Molecular Sequence Data ; Recombinant Fusion Proteins - analysis ; Viral Structural Proteins - analysis ; Viral Structural Proteins - genetics ; Virion - chemistry ; Virology</subject><ispartof>Journal of general virology, 1994-08, Vol.75 (8), p.2047-2052</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3537-2f029fc72d0bb61a109c15403398bd1c04e3bbe4b6c280c21dd06ec1f8c1e5bd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3744,3745,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4217794$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8046407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McLauchlan, John</creatorcontrib><creatorcontrib>Liefkens, Karin</creatorcontrib><creatorcontrib>Stow, Nigel D</creatorcontrib><title>The herpes simplex virus type 1 UL37 gene product is a component of virus particles</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, U.K.
and 2 International Agricultural College Larenstein, Marijkeweg 5, 6709 PE Wageningen, The Netherlands
The herpes simplex virus type 1 UL37 gene encodes a protein with an M r of 120K that is produced at late times after infection. To study the properties of this protein we have linked a 10 amino acid epitope derived from a human cytomegalovirus protein to the UL37 polypeptide coding sequences by inserting an oligonucleotide at a Spe I site that is unique in the virus genome and lies close to the 3' end of the open reading frame. From studies on the resultant virus recombinant using a monoclonal antibody that recognizes the inserted epitope we find that, contrary to a previous report, the UL37 protein is a structural component of both virions and L particles and is present in the tegument of virus particles. Indirect immunofluorescence analysis revealed that the protein is distributed throughout infected cells but is more abundant in the cytoplasm than the nucleus.
Received 5 January 1994;
accepted 17 February 1994.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Compartmentation</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>herpes simplex virus 1</subject><subject>Herpesvirus 1, Human - chemistry</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Recombinant Fusion Proteins - analysis</subject><subject>Viral Structural Proteins - analysis</subject><subject>Viral Structural Proteins - genetics</subject><subject>Virion - chemistry</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVpSLZpf0Ep6FBKL25GH5bsYwhtE1joIclZSPI4q-KvSHab_PtoWbM55jSCed6Z0UPIZwY_GNT1BQDnBRNMF7osqoKD1O_IhklV5nddvyebI3FGPqT0F4BJWepTclqBVBL0htze7ZDuME6YaAr91OET_Rfikuj8PCFl9H4rNH3AAekUx2bxMw2JWurHfhoHHGY6tmtgsnEOvsP0kZy0tkv4aa3n5P7Xz7ur62L75_fN1eW28KIUuuAt8Lr1mjfgnGI2_8mzUoIQdeUa5kGicA6lU55X4DlrGlDoWVt5hqVrxDn5dpibL3tcMM2mD8lj19kBxyUZrZTQIPibIFOKC1mKDIoD6OOYUsTWTDH0Nj4bBmbv3OyNmr1Ro0tTmb3znPqyjl9cj80xs0rO_a9r3yZvuzbawYd0xCRnWtcyY98P2C487P6HiCZr70M-xYXRZMevG18AJr2WKA</recordid><startdate>199408</startdate><enddate>199408</enddate><creator>McLauchlan, John</creator><creator>Liefkens, Karin</creator><creator>Stow, Nigel D</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199408</creationdate><title>The herpes simplex virus type 1 UL37 gene product is a component of virus particles</title><author>McLauchlan, John ; Liefkens, Karin ; Stow, Nigel D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3537-2f029fc72d0bb61a109c15403398bd1c04e3bbe4b6c280c21dd06ec1f8c1e5bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Compartmentation</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>herpes simplex virus 1</topic><topic>Herpesvirus 1, Human - chemistry</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Recombinant Fusion Proteins - analysis</topic><topic>Viral Structural Proteins - analysis</topic><topic>Viral Structural Proteins - genetics</topic><topic>Virion - chemistry</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McLauchlan, John</creatorcontrib><creatorcontrib>Liefkens, Karin</creatorcontrib><creatorcontrib>Stow, Nigel D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McLauchlan, John</au><au>Liefkens, Karin</au><au>Stow, Nigel D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The herpes simplex virus type 1 UL37 gene product is a component of virus particles</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1994-08</date><risdate>1994</risdate><volume>75</volume><issue>8</issue><spage>2047</spage><epage>2052</epage><pages>2047-2052</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>1 MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, U.K.
and 2 International Agricultural College Larenstein, Marijkeweg 5, 6709 PE Wageningen, The Netherlands
The herpes simplex virus type 1 UL37 gene encodes a protein with an M r of 120K that is produced at late times after infection. To study the properties of this protein we have linked a 10 amino acid epitope derived from a human cytomegalovirus protein to the UL37 polypeptide coding sequences by inserting an oligonucleotide at a Spe I site that is unique in the virus genome and lies close to the 3' end of the open reading frame. From studies on the resultant virus recombinant using a monoclonal antibody that recognizes the inserted epitope we find that, contrary to a previous report, the UL37 protein is a structural component of both virions and L particles and is present in the tegument of virus particles. Indirect immunofluorescence analysis revealed that the protein is distributed throughout infected cells but is more abundant in the cytoplasm than the nucleus.
Received 5 January 1994;
accepted 17 February 1994.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>8046407</pmid><doi>10.1099/0022-1317-75-8-2047</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1994-08, Vol.75 (8), p.2047-2052 |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Cell Compartmentation Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Genetics herpes simplex virus 1 Herpesvirus 1, Human - chemistry Microbiology Molecular Sequence Data Recombinant Fusion Proteins - analysis Viral Structural Proteins - analysis Viral Structural Proteins - genetics Virion - chemistry Virology |
| title | The herpes simplex virus type 1 UL37 gene product is a component of virus particles |
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