Hepatitis C: Description of a highly sensitive method for clinical detection of viral RNA

To improve the sensitivity of hepatitis C virus RNA (HCV RNA) detection in serum by ‘nested’ polymerase chain reaction (PCR), primers belonging to 5' non-coding (5'NC) regions were used to compare the classical phenol/chloroform technique by using the proteinase K and silica gel technique...

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Veröffentlicht in:	Journal of virological methods 1994-04, Vol.47 (1), p.83-94
Hauptverfasser:	Parvaz, Parviz, Guichard, Engracia, Chevallier, Philippe, Ritter, Jacques, Trepo, christian, Sepetjan, Maurice
Format:	Artikel
Sprache:	eng
Schlagworte:	5' non-coding primers Adult Base Sequence Biological and medical sciences Child Diagnostic Fundamental and applied biological sciences. Psychology Hepacivirus - isolation & purification Hepatitis C - blood Hepatitis C - microbiology hepatitis C virus Hepatitis C virus RNA detection Humans Microbiology Molecular Sequence Data Phenol/chloroform extraction Polymerase Chain Reaction - methods RNA, Viral - blood Sensitivity and Specificity Silica gel extraction Techniques used in virology Virology Virology - methods ‘Nested’ PCR
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