Evaluation of allergenic potency by REAST inhibition. A new tool for the standardization of allergenic extracts
The potency of allergenic extracts can be determined in vitro by RAST inhibition, and this has become the preferred method for the standardization of allergens. A disadvantage of this technique is the impossibility of obtaining data about allergens bound to the solid phase, i.e., the counterpart of...
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| Veröffentlicht in: | Journal of immunological methods 1994-08, Vol.173 (2), p.181-190 |
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| container_end_page | 190 |
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| container_title | Journal of immunological methods |
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| creator | Falagiani, Paolo Mistrello, Gianni Rapisarda, Giuseppina Festa, Adalgisa Cislaghi, Cinzia Zanoni, Dario |
| description | The potency of allergenic extracts can be determined in vitro by RAST inhibition, and this has become the preferred method for the standardization of allergens. A disadvantage of this technique is the impossibility of obtaining data about allergens bound to the solid phase, i.e., the counterpart of the inhibiting extract. The REAST (reverse the reaction of captured IgE with biotinylated allergen and the development of a colour reaction by subsequent addition of streptavidin-peroxidase and chromogenic substrate. The addition of an allergen extract in a dose-response fashion competes with the biotinylated allergen and inhibits the test. In the present study REAST inhibition has been evaluated with
Dermatophagoides pternyssinus, Parietaria judaica and mixed grass pollen extracts. The correlation of REAST inhibition with RAST inhibition and both intra-assay and inter-assay reproducibility have been evaluated. REAST inhibition is a potentially valuable new tool for the standardization of allergenic extracts. |
| doi_str_mv | 10.1016/0022-1759(94)90298-4 |
| format | Article |
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Dermatophagoides pternyssinus, Parietaria judaica and mixed grass pollen extracts. The correlation of REAST inhibition with RAST inhibition and both intra-assay and inter-assay reproducibility have been evaluated. REAST inhibition is a potentially valuable new tool for the standardization of allergenic extracts.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(94)90298-4</identifier><identifier>PMID: 8046253</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Allergen ; Allergenic potency ; Allergens - immunology ; Animals ; Binding, Competitive ; Dose-Response Relationship, Immunologic ; Humans ; IgE ; Immunoenzyme Techniques ; Immunoglobulin E - blood ; Immunoglobulin E - immunology ; Mites - immunology ; Poaceae - immunology ; Pollen - immunology ; Radio-allergosorbent test inhibition ; Radioallergosorbent Test ; Reference Standards ; Reproducibility of Results ; Reverse enzyme allergosorbent test inhibition</subject><ispartof>Journal of immunological methods, 1994-08, Vol.173 (2), p.181-190</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-9fd63fba200b1662b80fdf8596702627b310733e2ed376b896d92b1bb7f29bfd3</citedby><cites>FETCH-LOGICAL-c454t-9fd63fba200b1662b80fdf8596702627b310733e2ed376b896d92b1bb7f29bfd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-1759(94)90298-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8046253$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Falagiani, Paolo</creatorcontrib><creatorcontrib>Mistrello, Gianni</creatorcontrib><creatorcontrib>Rapisarda, Giuseppina</creatorcontrib><creatorcontrib>Festa, Adalgisa</creatorcontrib><creatorcontrib>Cislaghi, Cinzia</creatorcontrib><creatorcontrib>Zanoni, Dario</creatorcontrib><title>Evaluation of allergenic potency by REAST inhibition. A new tool for the standardization of allergenic extracts</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>The potency of allergenic extracts can be determined in vitro by RAST inhibition, and this has become the preferred method for the standardization of allergens. A disadvantage of this technique is the impossibility of obtaining data about allergens bound to the solid phase, i.e., the counterpart of the inhibiting extract. The REAST (reverse the reaction of captured IgE with biotinylated allergen and the development of a colour reaction by subsequent addition of streptavidin-peroxidase and chromogenic substrate. The addition of an allergen extract in a dose-response fashion competes with the biotinylated allergen and inhibits the test. In the present study REAST inhibition has been evaluated with
Dermatophagoides pternyssinus, Parietaria judaica and mixed grass pollen extracts. The correlation of REAST inhibition with RAST inhibition and both intra-assay and inter-assay reproducibility have been evaluated. REAST inhibition is a potentially valuable new tool for the standardization of allergenic extracts.</description><subject>Allergen</subject><subject>Allergenic potency</subject><subject>Allergens - immunology</subject><subject>Animals</subject><subject>Binding, Competitive</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Humans</subject><subject>IgE</subject><subject>Immunoenzyme Techniques</subject><subject>Immunoglobulin E - blood</subject><subject>Immunoglobulin E - immunology</subject><subject>Mites - immunology</subject><subject>Poaceae - immunology</subject><subject>Pollen - immunology</subject><subject>Radio-allergosorbent test inhibition</subject><subject>Radioallergosorbent Test</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Reverse enzyme allergosorbent test inhibition</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9vEzEUxC1EVULhG4DkE4LDlmd7115fkKIq_JEqVSrlbNnrZ2q0WQfbaQmfvhsS9YTg9A7zm3nSDCGvGJwzYPI9AOcNU51-q9t3Grjum_YJWbBe8UZp6J6SxSPyjDwv5QcAMJBwSk57aCXvxIKk1Z0dt7bGNNEUqB1HzN9xigPdpIrTsKNuR69Xy683NE630cU9eU6XdMJ7WlMaaUiZ1lukpdrJ2-zj77-l4a-a7VDLC3IS7Fjw5fGekW8fVzcXn5vLq09fLpaXzdB2bW108FIEZzmAY1Jy10Pwoe-0VMAlV04wUEIgRy-UdL2WXnPHnFOBaxe8OCNvDrmbnH5usVSzjmXAcbQTpm0xSkrRSqX-CzKplegVm8H2AA45lZIxmE2Oa5t3hoHZD2L2bZt920a35s8gpp1tr4_5W7dG_2g6LjDrHw46zm3cRcymDHEuHn3MOFTjU_z3gwdCfJqJ</recordid><startdate>19940801</startdate><enddate>19940801</enddate><creator>Falagiani, Paolo</creator><creator>Mistrello, Gianni</creator><creator>Rapisarda, Giuseppina</creator><creator>Festa, Adalgisa</creator><creator>Cislaghi, Cinzia</creator><creator>Zanoni, Dario</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19940801</creationdate><title>Evaluation of allergenic potency by REAST inhibition. A new tool for the standardization of allergenic extracts</title><author>Falagiani, Paolo ; Mistrello, Gianni ; Rapisarda, Giuseppina ; Festa, Adalgisa ; Cislaghi, Cinzia ; Zanoni, Dario</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-9fd63fba200b1662b80fdf8596702627b310733e2ed376b896d92b1bb7f29bfd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Allergen</topic><topic>Allergenic potency</topic><topic>Allergens - immunology</topic><topic>Animals</topic><topic>Binding, Competitive</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>Humans</topic><topic>IgE</topic><topic>Immunoenzyme Techniques</topic><topic>Immunoglobulin E - blood</topic><topic>Immunoglobulin E - immunology</topic><topic>Mites - immunology</topic><topic>Poaceae - immunology</topic><topic>Pollen - immunology</topic><topic>Radio-allergosorbent test inhibition</topic><topic>Radioallergosorbent Test</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Reverse enzyme allergosorbent test inhibition</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Falagiani, Paolo</creatorcontrib><creatorcontrib>Mistrello, Gianni</creatorcontrib><creatorcontrib>Rapisarda, Giuseppina</creatorcontrib><creatorcontrib>Festa, Adalgisa</creatorcontrib><creatorcontrib>Cislaghi, Cinzia</creatorcontrib><creatorcontrib>Zanoni, Dario</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Falagiani, Paolo</au><au>Mistrello, Gianni</au><au>Rapisarda, Giuseppina</au><au>Festa, Adalgisa</au><au>Cislaghi, Cinzia</au><au>Zanoni, Dario</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of allergenic potency by REAST inhibition. A new tool for the standardization of allergenic extracts</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1994-08-01</date><risdate>1994</risdate><volume>173</volume><issue>2</issue><spage>181</spage><epage>190</epage><pages>181-190</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>The potency of allergenic extracts can be determined in vitro by RAST inhibition, and this has become the preferred method for the standardization of allergens. A disadvantage of this technique is the impossibility of obtaining data about allergens bound to the solid phase, i.e., the counterpart of the inhibiting extract. The REAST (reverse the reaction of captured IgE with biotinylated allergen and the development of a colour reaction by subsequent addition of streptavidin-peroxidase and chromogenic substrate. The addition of an allergen extract in a dose-response fashion competes with the biotinylated allergen and inhibits the test. In the present study REAST inhibition has been evaluated with
Dermatophagoides pternyssinus, Parietaria judaica and mixed grass pollen extracts. The correlation of REAST inhibition with RAST inhibition and both intra-assay and inter-assay reproducibility have been evaluated. REAST inhibition is a potentially valuable new tool for the standardization of allergenic extracts.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8046253</pmid><doi>10.1016/0022-1759(94)90298-4</doi><tpages>10</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1994-08, Vol.173 (2), p.181-190 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Allergen Allergenic potency Allergens - immunology Animals Binding, Competitive Dose-Response Relationship, Immunologic Humans IgE Immunoenzyme Techniques Immunoglobulin E - blood Immunoglobulin E - immunology Mites - immunology Poaceae - immunology Pollen - immunology Radio-allergosorbent test inhibition Radioallergosorbent Test Reference Standards Reproducibility of Results Reverse enzyme allergosorbent test inhibition |
| title | Evaluation of allergenic potency by REAST inhibition. A new tool for the standardization of allergenic extracts |
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