The effect of egg rotation on the differentiation of primordial germ cells in Xenopus laevis

Eggs of X. laevis were rotated (sperm entrance point downwards) either through 90° (1×90 embryos) or 180° in two 90° steps (2×90 embryos) at approximately 25–30 min postfertilization after cooling to 13°C. The embryos were kept in their off-axis orientation and cooled until the early gastrul...

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Veröffentlicht in:Development (Cambridge) 1985-12, Vol.90 (1), p.79-99
Hauptverfasser: CLEINE, J. H, DIXON, K. E
Format: Artikel
Sprache:eng
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