Evaluation of Hepatic Metabolism of Salicylic Acid in Perfused Rat Liver

Evaluation of Hepatic Metabolism of Salicylic Acid in Perfused Rat Liver

Hepatic metabolism of salicylic acid was studied using a single-pass liver perfusion technique. Livers obtained from male, Sprague-Dawley rats (200–300 g) were perfused with Krebs-Henseleit bicarbonate buffer containing the drug (50–100 μg/mL) and glycine (0.5 or 5 mM) or glucose (5.5 mM) or bovine...

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Veröffentlicht in:Journal of pharmaceutical sciences 1994-04, Vol.83 (4), p.607-608
Hauptverfasser: Shetty, Bhasker V., Badr, Mostafa, Melethil, Srikumaran
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Glycine - metabolism
In Vitro Techniques
Liver - metabolism
Male
Oxygen Consumption - physiology
Perfusion
Rats
Rats, Sprague-Dawley
Salicylates - metabolism
Salicylic Acid
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container_title Journal of pharmaceutical sciences
container_volume 83
creator Shetty, Bhasker V.
Badr, Mostafa
Melethil, Srikumaran
description Hepatic metabolism of salicylic acid was studied using a single-pass liver perfusion technique. Livers obtained from male, Sprague-Dawley rats (200–300 g) were perfused with Krebs-Henseleit bicarbonate buffer containing the drug (50–100 μg/mL) and glycine (0.5 or 5 mM) or glucose (5.5 mM) or bovine serum albumin (0.33%). Effluent samples over a 30–50 min interval were analyzed for salicylic acid and its metabolites (gentisic acid, salicyluric acid, and salicyl acyl and salicyl phenolic glucuronides). Effluent concentrations of salicylic acid did not differ from those in the perfusate, indicating that the liver is not a site for the metabolism of this drug. Hepatic uptake studies are needed to confirm this finding.
doi_str_mv 10.1002/jps.2600830433
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subjects Animals
Glycine - metabolism
In Vitro Techniques
Liver - metabolism
Male
Oxygen Consumption - physiology
Perfusion
Rats
Rats, Sprague-Dawley
Salicylates - metabolism
Salicylic Acid
title Evaluation of Hepatic Metabolism of Salicylic Acid in Perfused Rat Liver
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