A gene-sized DNA molecule encoding the catalytic subunit of DNA polymerase alpha in the macronucleus of Oxytricha nova
We have isolated a gene-sized molecule encoding the catalytic subunit of DNA polymerase alpha from a macronuclear genomic library of Oxytricha nova, by using a 0.7-kb fragment of the corresponding human gene as a hybridization probe. Two different versions of the gene are present in the macronucleus...
Gespeichert in:
| Veröffentlicht in: | Gene 1994-07, Vol.144 (2), p.155-161 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 161 |
|---|---|
| container_issue | 2 |
| container_start_page | 155 |
| container_title | Gene |
| container_volume | 144 |
| creator | Mansour, S J Hoffman, D C Prescott, D M |
| description | We have isolated a gene-sized molecule encoding the catalytic subunit of DNA polymerase alpha from a macronuclear genomic library of Oxytricha nova, by using a 0.7-kb fragment of the corresponding human gene as a hybridization probe. Two different versions of the gene are present in the macronucleus, one with an EcoRI site (RI+) and one without an EcoRI site (RI-). The cloned RI- version has been characterized. It is 4938 bp in length, excluding telomeres. It consists of a 329-bp 5' leader, a 4479-bp coding region and a 130-bp 3' trailer. The deduced amino-acid sequence shares conserved regions with the yeast and human polypeptides. We also demonstrate by Southern analysis that gene-sized molecules of similar size, homologous to the isolated O. nova gene are present in the mac genome of closely and distantly related hypotrichs. |
| doi_str_mv | 10.1016/0378-1119(94)90373-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_76623279</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76623279</sourcerecordid><originalsourceid>FETCH-LOGICAL-p169t-e8ab0e59ca63a71ff66642bb290707d5a9fbfcf246b270cc5741072c0ab9025b3</originalsourceid><addsrcrecordid>eNqF0D1PwzAQBmAPoFIK_wAkTwiGgO0kdj1W5VOq6AJzZDuXNsixQxxXhF9PgIqVW0736rkbDqEzSq4pofyGpGKeUErlpcyu5DilSX6Apn_xEToO4Y2MledsgiZzkkpByBTtFngDDpJQf0KJb58XuPEWTLSAwRlf1m6D-y1go3plh742OEQdXd1jX_3w1tuhgU4FwMq2W4Vr97PQKNN5F42FGL7t-mPou9qMwPmdOkGHlbIBTvd9hl7v716Wj8lq_fC0XKySlnLZJzBXmkAujeKpErSqOOcZ05pJIogocyUrXZmKZVwzQYzJRUaJYIYoLQnLdTpDF793286_Rwh90dTBgLXKgY-hEJyzlAn5L6Scz6WgbITnexh1A2XRdnWjuqHYfzT9Ank9d3A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16689712</pqid></control><display><type>article</type><title>A gene-sized DNA molecule encoding the catalytic subunit of DNA polymerase alpha in the macronucleus of Oxytricha nova</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Mansour, S J ; Hoffman, D C ; Prescott, D M</creator><creatorcontrib>Mansour, S J ; Hoffman, D C ; Prescott, D M</creatorcontrib><description>We have isolated a gene-sized molecule encoding the catalytic subunit of DNA polymerase alpha from a macronuclear genomic library of Oxytricha nova, by using a 0.7-kb fragment of the corresponding human gene as a hybridization probe. Two different versions of the gene are present in the macronucleus, one with an EcoRI site (RI+) and one without an EcoRI site (RI-). The cloned RI- version has been characterized. It is 4938 bp in length, excluding telomeres. It consists of a 329-bp 5' leader, a 4479-bp coding region and a 130-bp 3' trailer. The deduced amino-acid sequence shares conserved regions with the yeast and human polypeptides. We also demonstrate by Southern analysis that gene-sized molecules of similar size, homologous to the isolated O. nova gene are present in the mac genome of closely and distantly related hypotrichs.</description><identifier>ISSN: 0378-1119</identifier><identifier>DOI: 10.1016/0378-1119(94)90373-5</identifier><identifier>PMID: 8039700</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Base Sequence ; Binding Sites - genetics ; Biological Evolution ; Cell Nucleus - enzymology ; Cloning, Molecular ; Conserved Sequence ; DNA Polymerase II - genetics ; DNA, Protozoan ; Humans ; Molecular Sequence Data ; Oxytricha - enzymology ; Oxytricha nova ; Sequence Homology, Amino Acid</subject><ispartof>Gene, 1994-07, Vol.144 (2), p.155-161</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8039700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mansour, S J</creatorcontrib><creatorcontrib>Hoffman, D C</creatorcontrib><creatorcontrib>Prescott, D M</creatorcontrib><title>A gene-sized DNA molecule encoding the catalytic subunit of DNA polymerase alpha in the macronucleus of Oxytricha nova</title><title>Gene</title><addtitle>Gene</addtitle><description>We have isolated a gene-sized molecule encoding the catalytic subunit of DNA polymerase alpha from a macronuclear genomic library of Oxytricha nova, by using a 0.7-kb fragment of the corresponding human gene as a hybridization probe. Two different versions of the gene are present in the macronucleus, one with an EcoRI site (RI+) and one without an EcoRI site (RI-). The cloned RI- version has been characterized. It is 4938 bp in length, excluding telomeres. It consists of a 329-bp 5' leader, a 4479-bp coding region and a 130-bp 3' trailer. The deduced amino-acid sequence shares conserved regions with the yeast and human polypeptides. We also demonstrate by Southern analysis that gene-sized molecules of similar size, homologous to the isolated O. nova gene are present in the mac genome of closely and distantly related hypotrichs.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites - genetics</subject><subject>Biological Evolution</subject><subject>Cell Nucleus - enzymology</subject><subject>Cloning, Molecular</subject><subject>Conserved Sequence</subject><subject>DNA Polymerase II - genetics</subject><subject>DNA, Protozoan</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Oxytricha - enzymology</subject><subject>Oxytricha nova</subject><subject>Sequence Homology, Amino Acid</subject><issn>0378-1119</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0D1PwzAQBmAPoFIK_wAkTwiGgO0kdj1W5VOq6AJzZDuXNsixQxxXhF9PgIqVW0736rkbDqEzSq4pofyGpGKeUErlpcyu5DilSX6Apn_xEToO4Y2MledsgiZzkkpByBTtFngDDpJQf0KJb58XuPEWTLSAwRlf1m6D-y1go3plh742OEQdXd1jX_3w1tuhgU4FwMq2W4Vr97PQKNN5F42FGL7t-mPou9qMwPmdOkGHlbIBTvd9hl7v716Wj8lq_fC0XKySlnLZJzBXmkAujeKpErSqOOcZ05pJIogocyUrXZmKZVwzQYzJRUaJYIYoLQnLdTpDF793286_Rwh90dTBgLXKgY-hEJyzlAn5L6Scz6WgbITnexh1A2XRdnWjuqHYfzT9Ank9d3A</recordid><startdate>19940708</startdate><enddate>19940708</enddate><creator>Mansour, S J</creator><creator>Hoffman, D C</creator><creator>Prescott, D M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19940708</creationdate><title>A gene-sized DNA molecule encoding the catalytic subunit of DNA polymerase alpha in the macronucleus of Oxytricha nova</title><author>Mansour, S J ; Hoffman, D C ; Prescott, D M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p169t-e8ab0e59ca63a71ff66642bb290707d5a9fbfcf246b270cc5741072c0ab9025b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Binding Sites - genetics</topic><topic>Biological Evolution</topic><topic>Cell Nucleus - enzymology</topic><topic>Cloning, Molecular</topic><topic>Conserved Sequence</topic><topic>DNA Polymerase II - genetics</topic><topic>DNA, Protozoan</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Oxytricha - enzymology</topic><topic>Oxytricha nova</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mansour, S J</creatorcontrib><creatorcontrib>Hoffman, D C</creatorcontrib><creatorcontrib>Prescott, D M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mansour, S J</au><au>Hoffman, D C</au><au>Prescott, D M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A gene-sized DNA molecule encoding the catalytic subunit of DNA polymerase alpha in the macronucleus of Oxytricha nova</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1994-07-08</date><risdate>1994</risdate><volume>144</volume><issue>2</issue><spage>155</spage><epage>161</epage><pages>155-161</pages><issn>0378-1119</issn><abstract>We have isolated a gene-sized molecule encoding the catalytic subunit of DNA polymerase alpha from a macronuclear genomic library of Oxytricha nova, by using a 0.7-kb fragment of the corresponding human gene as a hybridization probe. Two different versions of the gene are present in the macronucleus, one with an EcoRI site (RI+) and one without an EcoRI site (RI-). The cloned RI- version has been characterized. It is 4938 bp in length, excluding telomeres. It consists of a 329-bp 5' leader, a 4479-bp coding region and a 130-bp 3' trailer. The deduced amino-acid sequence shares conserved regions with the yeast and human polypeptides. We also demonstrate by Southern analysis that gene-sized molecules of similar size, homologous to the isolated O. nova gene are present in the mac genome of closely and distantly related hypotrichs.</abstract><cop>Netherlands</cop><pmid>8039700</pmid><doi>10.1016/0378-1119(94)90373-5</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1994-07, Vol.144 (2), p.155-161 |
| issn | 0378-1119 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76623279 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Base Sequence Binding Sites - genetics Biological Evolution Cell Nucleus - enzymology Cloning, Molecular Conserved Sequence DNA Polymerase II - genetics DNA, Protozoan Humans Molecular Sequence Data Oxytricha - enzymology Oxytricha nova Sequence Homology, Amino Acid |
| title | A gene-sized DNA molecule encoding the catalytic subunit of DNA polymerase alpha in the macronucleus of Oxytricha nova |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T16%3A33%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20gene-sized%20DNA%20molecule%20encoding%20the%20catalytic%20subunit%20of%20DNA%20polymerase%20alpha%20in%20the%20macronucleus%20of%20Oxytricha%20nova&rft.jtitle=Gene&rft.au=Mansour,%20S%20J&rft.date=1994-07-08&rft.volume=144&rft.issue=2&rft.spage=155&rft.epage=161&rft.pages=155-161&rft.issn=0378-1119&rft_id=info:doi/10.1016/0378-1119(94)90373-5&rft_dat=%3Cproquest_pubme%3E76623279%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16689712&rft_id=info:pmid/8039700&rfr_iscdi=true |