Developmentally regulated expression during gametogenesis of the murine gene meg1 suggests a role in meiosis

Previous studies have shown that in adult male mice, expression of the meg1 gene is restricted to meiotic and early postmeiotic testicular germ cells. We have now analyzed the expression of meg1 during postnatal testicular development and the comparable meiotic stages in the female. The 0.75 kb tran...

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Veröffentlicht in:Molecular reproduction and development 1994-05, Vol.38 (1), p.16-23
Hauptverfasser: Don, Jeremy, Winer, Martin A., Wolgemuth, Debra J.
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Wolgemuth, Debra J.
description Previous studies have shown that in adult male mice, expression of the meg1 gene is restricted to meiotic and early postmeiotic testicular germ cells. We have now analyzed the expression of meg1 during postnatal testicular development and the comparable meiotic stages in the female. The 0.75 kb transcript for meg1 begins to accumulate in testes at d8‐9 of postnatal (pn) development, coincident with the entry of germ cells into meiosis, and is expressed most abundantly at pn d14 and subsequent stages, when the spermatocytes have entered pachytene. In situ hybridization analysis shows that meg1 is expressed at very low levels in leptotene cells and increases as the cells progress through zygotene and pachytene stages. In the embryonic ovary, meg1 is not detected until after day 15 of gestation when the cells have entered the pachytene stage of meiosis I. In situ hybridization analysis suggests that meg1 transcripts are expressed at higher levels in degenerating rather than in healthy pachytene stage oocytes; meg1 is not expressed in any cells of the adult ovary, regardless of the stage of follicular development. These results suggest that meg1 is indeed a meiosis‐associated gene in both male and female germ cells through the pachytene stage of meiosis I and appears to exhibit sex‐specific differences in its expression thereafter. © 1994 Wiley‐Liss, Inc.
doi_str_mv 10.1002/mrd.1080380104
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We have now analyzed the expression of meg1 during postnatal testicular development and the comparable meiotic stages in the female. The 0.75 kb transcript for meg1 begins to accumulate in testes at d8‐9 of postnatal (pn) development, coincident with the entry of germ cells into meiosis, and is expressed most abundantly at pn d14 and subsequent stages, when the spermatocytes have entered pachytene. In situ hybridization analysis shows that meg1 is expressed at very low levels in leptotene cells and increases as the cells progress through zygotene and pachytene stages. In the embryonic ovary, meg1 is not detected until after day 15 of gestation when the cells have entered the pachytene stage of meiosis I. In situ hybridization analysis suggests that meg1 transcripts are expressed at higher levels in degenerating rather than in healthy pachytene stage oocytes; meg1 is not expressed in any cells of the adult ovary, regardless of the stage of follicular development. 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Psychology ; Gene expression ; Gene Expression Regulation ; Gestational Age ; GRB10 Adaptor Protein ; In Situ Hybridization ; Male ; Mammalian germ cell differentiation ; Meiosis ; Meiosis - genetics ; Mice ; Nuclear Proteins ; Ovary - embryology ; Ovary - metabolism ; Phosphoproteins ; Proteins - genetics ; Sex Characteristics ; Sexual differentiation and maturation. Puberty. 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Reprod. Dev</addtitle><description>Previous studies have shown that in adult male mice, expression of the meg1 gene is restricted to meiotic and early postmeiotic testicular germ cells. We have now analyzed the expression of meg1 during postnatal testicular development and the comparable meiotic stages in the female. The 0.75 kb transcript for meg1 begins to accumulate in testes at d8‐9 of postnatal (pn) development, coincident with the entry of germ cells into meiosis, and is expressed most abundantly at pn d14 and subsequent stages, when the spermatocytes have entered pachytene. In situ hybridization analysis shows that meg1 is expressed at very low levels in leptotene cells and increases as the cells progress through zygotene and pachytene stages. In the embryonic ovary, meg1 is not detected until after day 15 of gestation when the cells have entered the pachytene stage of meiosis I. In situ hybridization analysis suggests that meg1 transcripts are expressed at higher levels in degenerating rather than in healthy pachytene stage oocytes; meg1 is not expressed in any cells of the adult ovary, regardless of the stage of follicular development. These results suggest that meg1 is indeed a meiosis‐associated gene in both male and female germ cells through the pachytene stage of meiosis I and appears to exhibit sex‐specific differences in its expression thereafter. © 1994 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Cycle Proteins</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Gestational Age</subject><subject>GRB10 Adaptor Protein</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Mammalian germ cell differentiation</subject><subject>Meiosis</subject><subject>Meiosis - genetics</subject><subject>Mice</subject><subject>Nuclear Proteins</subject><subject>Ovary - embryology</subject><subject>Ovary - metabolism</subject><subject>Phosphoproteins</subject><subject>Proteins - genetics</subject><subject>Sex Characteristics</subject><subject>Sexual differentiation and maturation. Puberty. Climacterium</subject><subject>Space life sciences</subject><subject>Spermatogenesis - genetics</subject><subject>Testis - embryology</subject><subject>Testis - growth &amp; development</subject><subject>Testis - metabolism</subject><subject>Vertebrates: reproduction</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxSMEKqVw5YbkA-KWYscfsY-oCy1SKRUCgbhYU2cSDE682Ant_vd4tatFnHryaN7vjcd-VfWc0VNGafN6TF0pNOWaMioeVMeMGl03rZEPt7WgtZDNt8fVk5x_UkqN0fSoOtJUGKrocRVW-AdDXI84zRDChiQclgAzdgTv1glz9nEi3ZL8NJABRpzjgBNmn0nsyfwDybjVkGy7ZMSBkbwMA-Y5EyApBiR-Kn0fi-Vp9aiHkPHZ_jypvrx7-_nsor78eP7-7M1l7SQVopZOOHHTNAzAUS0McNao8iJoHYIDzsH1BpTsBXbMSHCGGyY7IZC1nTaOn1SvdnPXKf5eyi529NlhCDBhXLJtlWqYkuZekCmtJWeigKc70KWYc8LerpMfIW0so3abgy052H85FMOL_eTlZsTugO8_vugv9zpkB6FPMDmfD5hgUjScFczssFsfcHPPpfbDp9V_K9Q7r88z3h28kH5Z1fJW2q9X59Zct1dSra7td_4X77yxew</recordid><startdate>199405</startdate><enddate>199405</enddate><creator>Don, Jeremy</creator><creator>Winer, Martin A.</creator><creator>Wolgemuth, Debra J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199405</creationdate><title>Developmentally regulated expression during gametogenesis of the murine gene meg1 suggests a role in meiosis</title><author>Don, Jeremy ; Winer, Martin A. ; Wolgemuth, Debra J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5044-5c4c4b221aac0849a3126279a7ceaca33acf9a65f4ed195ac93915d44e17d89c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Cycle Proteins</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Gestational Age</topic><topic>GRB10 Adaptor Protein</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Mammalian germ cell differentiation</topic><topic>Meiosis</topic><topic>Meiosis - genetics</topic><topic>Mice</topic><topic>Nuclear Proteins</topic><topic>Ovary - embryology</topic><topic>Ovary - metabolism</topic><topic>Phosphoproteins</topic><topic>Proteins - genetics</topic><topic>Sex Characteristics</topic><topic>Sexual differentiation and maturation. Puberty. Climacterium</topic><topic>Space life sciences</topic><topic>Spermatogenesis - genetics</topic><topic>Testis - embryology</topic><topic>Testis - growth &amp; development</topic><topic>Testis - metabolism</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Don, Jeremy</creatorcontrib><creatorcontrib>Winer, Martin A.</creatorcontrib><creatorcontrib>Wolgemuth, Debra J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Don, Jeremy</au><au>Winer, Martin A.</au><au>Wolgemuth, Debra J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmentally regulated expression during gametogenesis of the murine gene meg1 suggests a role in meiosis</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>1994-05</date><risdate>1994</risdate><volume>38</volume><issue>1</issue><spage>16</spage><epage>23</epage><pages>16-23</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>Previous studies have shown that in adult male mice, expression of the meg1 gene is restricted to meiotic and early postmeiotic testicular germ cells. We have now analyzed the expression of meg1 during postnatal testicular development and the comparable meiotic stages in the female. The 0.75 kb transcript for meg1 begins to accumulate in testes at d8‐9 of postnatal (pn) development, coincident with the entry of germ cells into meiosis, and is expressed most abundantly at pn d14 and subsequent stages, when the spermatocytes have entered pachytene. In situ hybridization analysis shows that meg1 is expressed at very low levels in leptotene cells and increases as the cells progress through zygotene and pachytene stages. In the embryonic ovary, meg1 is not detected until after day 15 of gestation when the cells have entered the pachytene stage of meiosis I. In situ hybridization analysis suggests that meg1 transcripts are expressed at higher levels in degenerating rather than in healthy pachytene stage oocytes; meg1 is not expressed in any cells of the adult ovary, regardless of the stage of follicular development. These results suggest that meg1 is indeed a meiosis‐associated gene in both male and female germ cells through the pachytene stage of meiosis I and appears to exhibit sex‐specific differences in its expression thereafter. © 1994 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8049060</pmid><doi>10.1002/mrd.1080380104</doi><tpages>8</tpages></addata></record>
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subjects Animals
Biological and medical sciences
Cell Cycle Proteins
Female
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation
Gestational Age
GRB10 Adaptor Protein
In Situ Hybridization
Male
Mammalian germ cell differentiation
Meiosis
Meiosis - genetics
Mice
Nuclear Proteins
Ovary - embryology
Ovary - metabolism
Phosphoproteins
Proteins - genetics
Sex Characteristics
Sexual differentiation and maturation. Puberty. Climacterium
Space life sciences
Spermatogenesis - genetics
Testis - embryology
Testis - growth & development
Testis - metabolism
Vertebrates: reproduction
title Developmentally regulated expression during gametogenesis of the murine gene meg1 suggests a role in meiosis
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