Efficient interaction of recA protein with fluorescent dye-labeled oligonucleotides
Some fluorescein derivatives attached to the 5′-end of oligonucleotides stimulate recA protein—oligonucleotide binding. The complex formation at near stoichiometric DNA/protein ratios is demonstrated for 18-bases-long oligonucleotides. The complexes with dye-labeled oligonucleotides are shown to be...
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| Veröffentlicht in: | FEBS letters 1994-07, Vol.349 (1), p.65-68 |
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| creator | Volodin, Alexander A. Smirnova, Helen A. Bocharova, Tatjana N. |
| description | Some fluorescein derivatives attached to the 5′-end of oligonucleotides stimulate recA protein—oligonucleotide binding. The complex formation at near stoichiometric DNA/protein ratios is demonstrated for 18-bases-long oligonucleotides. The complexes with dye-labeled oligonucleotides are shown to be active in the reaction of homologous strand exchange. The strand exchange reaction in the presence of adenosine-5′-
O-(3-thiotriphosphate) proceeds with the formation of a stable complex of recA protein with the double stranded oligonucleotide, which is a product of the strand exchange. The displaced single-stranded oligonucleotide is shown to be bound weakly. |
| doi_str_mv | 10.1016/0014-5793(94)00640-7 |
| format | Article |
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O-(3-thiotriphosphate) proceeds with the formation of a stable complex of recA protein with the double stranded oligonucleotide, which is a product of the strand exchange. The displaced single-stranded oligonucleotide is shown to be bound weakly.</description><subject>ATP-S, adenosine-5′-O-(3-thiotriphosphate)</subject><subject>b., base</subject><subject>Base Sequence</subject><subject>bp, base pair</subject><subject>DNA, Single-Stranded - metabolism</subject><subject>ds-, ss-, double- and single stranded dye-labeled oligonucleotides respectively</subject><subject>ds-, ss-, double- and single-stranded unmodified oligonucleotides respectively</subject><subject>Fluoresceins - metabolism</subject><subject>Fluorescent dye-labeled oligonucleotide</subject><subject>Fluorescent Dyes - metabolism</subject><subject>JOE, 4′,5′-dichloro-2′,7′-dimethoxy-6-carboxy-fluorescein</subject><subject>Molecular Sequence Data</subject><subject>Oligodeoxyribonucleotides - metabolism</subject><subject>PAGE, polyacryl-amide gel electrophoresis</subject><subject>Rec A Recombinases - metabolism</subject><subject>recA protein</subject><subject>recA-oligonucleotide complex</subject><subject>Recombination, Genetic</subject><subject>SDS, dodecyl sulfate sodium salt</subject><subject>Strand exchange</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFP3DAQha0KBMvSf1CknFA5BOxkYjsXJIp2aSUkDi1nK3HGxcgbb-2kaP89zu5qj6Uny_PevBl9Q8gXRq8ZZfyGUgZ5Jeryaw1XlHKgufhEZkyKMi-ByyMyO1hOyVmMrzT9JatPyImkUJUUZuTnwhirLfZDZvsBQ6MH6_vMmyygvsvWwQ9o--zNDi-ZcaMPGPVk7jaYu6ZFh13mnf3t-1E79IPtMJ6TY9O4iJ_375w8Lxe_7r_nj08PP-7vHnMNtRC5FHWNXAtjWFNwNKZtqq4AKQrZVLzqqOTAuSxBtNyAAaiMbI1B0IUuipKWc3K5y01b_hkxDmpl03bONT36MSrBOYOClR8aGRdUpGHJCDujDj7GgEatg101YaMYVRN0NRFVE1FVg9pCVyK1Xezzx3aF3aFpTznpy53-Zh1u_itTLRffikmY6jVsq9Og210QJqx_LQYVp9tp7Gw616A6b_-96TsMBqQk</recordid><startdate>19940725</startdate><enddate>19940725</enddate><creator>Volodin, Alexander A.</creator><creator>Smirnova, Helen A.</creator><creator>Bocharova, Tatjana N.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19940725</creationdate><title>Efficient interaction of recA protein with fluorescent dye-labeled oligonucleotides</title><author>Volodin, Alexander A. ; 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The complex formation at near stoichiometric DNA/protein ratios is demonstrated for 18-bases-long oligonucleotides. The complexes with dye-labeled oligonucleotides are shown to be active in the reaction of homologous strand exchange. The strand exchange reaction in the presence of adenosine-5′-
O-(3-thiotriphosphate) proceeds with the formation of a stable complex of recA protein with the double stranded oligonucleotide, which is a product of the strand exchange. The displaced single-stranded oligonucleotide is shown to be bound weakly.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>8045304</pmid><doi>10.1016/0014-5793(94)00640-7</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1994-07, Vol.349 (1), p.65-68 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76614213 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | ATP-S, adenosine-5′-O-(3-thiotriphosphate) b., base Base Sequence bp, base pair DNA, Single-Stranded - metabolism ds-, ss-, double- and single stranded dye-labeled oligonucleotides respectively ds-, ss-, double- and single-stranded unmodified oligonucleotides respectively Fluoresceins - metabolism Fluorescent dye-labeled oligonucleotide Fluorescent Dyes - metabolism JOE, 4′,5′-dichloro-2′,7′-dimethoxy-6-carboxy-fluorescein Molecular Sequence Data Oligodeoxyribonucleotides - metabolism PAGE, polyacryl-amide gel electrophoresis Rec A Recombinases - metabolism recA protein recA-oligonucleotide complex Recombination, Genetic SDS, dodecyl sulfate sodium salt Strand exchange |
| title | Efficient interaction of recA protein with fluorescent dye-labeled oligonucleotides |
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