Construction of novel shuttle vectors and a cosmid vector for the glutamic acid-producing bacteria Brevibacterium lactofermentum and Corynebacterium glutamicum

Novel cloning vectors for glutamic acid-producing bacteria have been constructed. Two cryptic plasmids, pAM330 from Brevibacterium lactofermentum and pHM1519 from Corynebacterium glutamicum, were used as precursors, and recombined with pBR325 or pUB110. Resultant composite plasmids were able to prop...

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Veröffentlicht in:	Gene 1985, Vol.39 (2), p.281-286
Hauptverfasser:	Kiyoshi, Miwa, Kazuhiko, Matsui, Mahito, Terabe, Koichi, Ito, Masaaki, Ishida, Hiroshi, Takagi, Shigeru, Nakamori, Konosuke, Sano
Format:	Artikel
Sprache:	eng
Schlagworte:	bacteriophage flA Biological and medical sciences Biotechnology Brevibacterium - genetics Chromosome Mapping cohesive end Corynebacterium - genetics Cosmids Food industries Food microbiology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Vectors Glutamates - metabolism Methods. Procedures. Technologies pBR325 Plasmids pUB110 Recombinant DNA transduction Transduction, Genetic Vectors (cloning, transfer, expression). Insertion sequences and transposons
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