Purification of the human anionic polypeptide fraction of the apo‐bile lipoprotein complex by zonal ultracentrifugation
The two main proteic constituents of the human Apo‐bile lipoprotein complex (BLC), i.e., the anionic polypeptide fraction (APF) and the IgA fragments, were separated by preparative zonal ultracentrifugation using a sucrose gradient containing 1.5 mM glycodesoxycholate. The purification of the APF wa...
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| Veröffentlicht in: | Lipids 1985-12, Vol.20 (12), p.884-889 |
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| container_title | Lipids |
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| creator | Martigne, M. Domingo, N. Lafont, H. Nalbone, G. Hauton, J. C. |
| description | The two main proteic constituents of the human Apo‐bile lipoprotein complex (BLC), i.e., the anionic polypeptide fraction (APF) and the IgA fragments, were separated by preparative zonal ultracentrifugation using a sucrose gradient containing 1.5 mM glycodesoxycholate. The purification of the APF was verified by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis and immunology, and its amino acid composition then was determined. This procedure was used to obtain a polyclonal antiserum directed solely against the APF. |
| doi_str_mv | 10.1007/BF02534772 |
| format | Article |
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This procedure was used to obtain a polyclonal antiserum directed solely against the APF.</description><identifier>ISSN: 0024-4201</identifier><identifier>EISSN: 1558-9307</identifier><identifier>DOI: 10.1007/BF02534772</identifier><identifier>PMID: 3937955</identifier><identifier>CODEN: LPDSAP</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer‐Verlag</publisher><subject>Amino Acids - analysis ; Analysis of complex biological substances ; Analytical, structural and metabolic biochemistry ; Apolipoproteins - isolation & purification ; Bile - analysis ; Biological and medical sciences ; Blood and biological fluids ; Centrifugation, Zonal - methods ; Fundamental and applied biological sciences. 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C.</creatorcontrib><title>Purification of the human anionic polypeptide fraction of the apo‐bile lipoprotein complex by zonal ultracentrifugation</title><title>Lipids</title><addtitle>Lipids</addtitle><description>The two main proteic constituents of the human Apo‐bile lipoprotein complex (BLC), i.e., the anionic polypeptide fraction (APF) and the IgA fragments, were separated by preparative zonal ultracentrifugation using a sucrose gradient containing 1.5 mM glycodesoxycholate. The purification of the APF was verified by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis and immunology, and its amino acid composition then was determined. This procedure was used to obtain a polyclonal antiserum directed solely against the APF.</description><subject>Amino Acids - analysis</subject><subject>Analysis of complex biological substances</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Apolipoproteins - isolation & purification</subject><subject>Bile - analysis</subject><subject>Biological and medical sciences</subject><subject>Blood and biological fluids</subject><subject>Centrifugation, Zonal - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immune Sera</subject><subject>Immunodiffusion</subject><subject>Molecular Weight</subject><subject>Peptides - isolation & purification</subject><issn>0024-4201</issn><issn>1558-9307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFO3DAQhi0EgmXphTuSD1UPSEvt2I7jY6HdgrRSOdBzZHvHxZUThzgRTU99BJ6RJ8F0V8CJk2XP5_lnPoSOKTmjhMjP50tSCMalLHbQjApRLRQjchfNCCn4gheEHqDDlH7nK-VK7KN9pphUQszQdD323nmrBx9bHB0ebgHfjo1usW7zk7e4i2HqoBv8GrDrtX1L6i4-_nswPgAOvotdHwfwLbax6QL8wWbCf2OrAx7DkH9CO-Sw8df_sCO053RI8GF7ztHP5bebi8vF6sf3q4svq4VlIk-vNOd5SVEpUZlKGW64FkoBLYxxzAopONdVuVbWWOmoBK5KWhAowFHhiGJz9GnTNw93N0Ia6sYnCyHoFuKYalmWhGQfGTzdgLaPKfXg6q73je6nmpL62XP96jnDJ9uuo2lg_YJuxeb6x21dJ6tD9tZan16wSgomSJkxssHus8LpncB6dXX9lVQVZ0_Bw5WU</recordid><startdate>198512</startdate><enddate>198512</enddate><creator>Martigne, M.</creator><creator>Domingo, N.</creator><creator>Lafont, H.</creator><creator>Nalbone, G.</creator><creator>Hauton, J. 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Psychology</topic><topic>Humans</topic><topic>Immune Sera</topic><topic>Immunodiffusion</topic><topic>Molecular Weight</topic><topic>Peptides - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martigne, M.</creatorcontrib><creatorcontrib>Domingo, N.</creatorcontrib><creatorcontrib>Lafont, H.</creatorcontrib><creatorcontrib>Nalbone, G.</creatorcontrib><creatorcontrib>Hauton, J. 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C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of the human anionic polypeptide fraction of the apo‐bile lipoprotein complex by zonal ultracentrifugation</atitle><jtitle>Lipids</jtitle><addtitle>Lipids</addtitle><date>1985-12</date><risdate>1985</risdate><volume>20</volume><issue>12</issue><spage>884</spage><epage>889</epage><pages>884-889</pages><issn>0024-4201</issn><eissn>1558-9307</eissn><coden>LPDSAP</coden><abstract>The two main proteic constituents of the human Apo‐bile lipoprotein complex (BLC), i.e., the anionic polypeptide fraction (APF) and the IgA fragments, were separated by preparative zonal ultracentrifugation using a sucrose gradient containing 1.5 mM glycodesoxycholate. The purification of the APF was verified by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis and immunology, and its amino acid composition then was determined. This procedure was used to obtain a polyclonal antiserum directed solely against the APF.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer‐Verlag</pub><pmid>3937955</pmid><doi>10.1007/BF02534772</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0024-4201 |
| ispartof | Lipids, 1985-12, Vol.20 (12), p.884-889 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Amino Acids - analysis Analysis of complex biological substances Analytical, structural and metabolic biochemistry Apolipoproteins - isolation & purification Bile - analysis Biological and medical sciences Blood and biological fluids Centrifugation, Zonal - methods Fundamental and applied biological sciences. Psychology Humans Immune Sera Immunodiffusion Molecular Weight Peptides - isolation & purification |
| title | Purification of the human anionic polypeptide fraction of the apo‐bile lipoprotein complex by zonal ultracentrifugation |
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