Construction of multipurpose gene cartridges based on a novel synthetic promoter for high-level gene expression in Gram-negative bacteria

A series of gene cartridges containing a novel synthetic promoter ( P syn) was constructed. The P syn sequence is based on the consensus of a number of naturally occurring promoters and displays strong activity in Escherichia coli and Rhizobium leguminosarum. In a direct comparison, P syn proved to...

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