Influence of antibody and complement components on phagocytosis and chemiluminescence of macrophages
Macrophages are known to release reactive oxygen species (O 2 −, 1O 2, H 2O 2, OH·) in response to various membrane stimuliHowever, our studies show that phagocytic stimulation of macrophages is not necessarily accompanied by a stimulation of the oxidative burstWhereas IgG-opsonized erythrocytes wer...
Gespeichert in:
| Veröffentlicht in: | Immunobiology (1979) 1985-12, Vol.170 (5), p.390-401 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 401 |
|---|---|
| container_issue | 5 |
| container_start_page | 390 |
| container_title | Immunobiology (1979) |
| container_volume | 170 |
| creator | Büscher, K.-H. Klimetzek, V. Opferkuch, W. |
| description | Macrophages are known to release reactive oxygen species (O
2
−,
1O
2, H
2O
2, OH·) in response to various membrane stimuliHowever, our studies show that phagocytic stimulation of macrophages is not necessarily accompanied by a stimulation of the oxidative burstWhereas IgG-opsonized erythrocytes were capable to induce phagocytosis and a chemiluminescence response, both being dependent on the number of IgG bound per erythrocyte, C3b-bearing erythrocytes were well ingested but failed to induce any chemiluminescence reactionFurthermore, stimulation of macrophages, via the Fc-receptors, seems to alter their functional state in regard to the activation of a receptor, which enables them to recognize membrane lesions on the target erythrocyteThe presence of IgG and membrane lesions, e.gthe C5b-9-complex of complement, induced a marked increase in chemiluminescence compared with stimulation by IgG-bearing particles aloneThe augmented response of macrophages was at least in part due to an additional release of H
2O
2, which was not liberated in response to IgG-bearing erythrocytesThis «Alesion recognizing receptor» in the macrophage membrane could not be activated by stimulation of C3b-receptors, indicating its functional linkage to the Fc-receptors. |
| doi_str_mv | 10.1016/S0171-2985(85)80063-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76593444</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0171298585800632</els_id><sourcerecordid>76593444</sourcerecordid><originalsourceid>FETCH-LOGICAL-c389t-fd64ce812e670465bd2e2c93320468feb600a8597df1a98015bfbe50a122f3403</originalsourceid><addsrcrecordid>eNqFkE1r3DAQhkVpSDbb_oSAD6WkByf6sPVxCiWkSWAhh7RnIUujRsGWNpZd2H9fObvda0GgEfPM6OVB6ILgK4IJv37GRJCaKtleyvabxJizmn5AKyKFrBkV6iNaHZEzdJ7zK8ZEUSFP0SlVjHPBV8g9Rt_PEC1UyVcmTqFLblcKV9k0bHsYIE7vZYqlylWK1fbF_E52N6Uc8p58gSH08xAiZPtv12DsmBYU8id04k2f4fPhXqNfP-5-3j7Um6f7x9vvm9oyqabaO95YkIQCF7jhbecoUKsYo-UlPXQcYyNbJZwnRklM2s530GJDKPWswWyNvu73bsf0NkOe9BBKoL43EdKcteCtYk3TFLDdgyViziN4vR3DYMadJlgvdvW7Xb2o08tZ7Gpa5i4OH8zdAO44ddBZ-l8OfZOt6f1oog35iElJGspEwW72GBQZfwKMOtuweHNhBDtpl8J_gvwF3uWX2Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76593444</pqid></control><display><type>article</type><title>Influence of antibody and complement components on phagocytosis and chemiluminescence of macrophages</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Büscher, K.-H. ; Klimetzek, V. ; Opferkuch, W.</creator><creatorcontrib>Büscher, K.-H. ; Klimetzek, V. ; Opferkuch, W.</creatorcontrib><description>Macrophages are known to release reactive oxygen species (O
2
−,
1O
2, H
2O
2, OH·) in response to various membrane stimuliHowever, our studies show that phagocytic stimulation of macrophages is not necessarily accompanied by a stimulation of the oxidative burstWhereas IgG-opsonized erythrocytes were capable to induce phagocytosis and a chemiluminescence response, both being dependent on the number of IgG bound per erythrocyte, C3b-bearing erythrocytes were well ingested but failed to induce any chemiluminescence reactionFurthermore, stimulation of macrophages, via the Fc-receptors, seems to alter their functional state in regard to the activation of a receptor, which enables them to recognize membrane lesions on the target erythrocyteThe presence of IgG and membrane lesions, e.gthe C5b-9-complex of complement, induced a marked increase in chemiluminescence compared with stimulation by IgG-bearing particles aloneThe augmented response of macrophages was at least in part due to an additional release of H
2O
2, which was not liberated in response to IgG-bearing erythrocytesThis «Alesion recognizing receptor» in the macrophage membrane could not be activated by stimulation of C3b-receptors, indicating its functional linkage to the Fc-receptors.</description><identifier>ISSN: 0171-2985</identifier><identifier>EISSN: 1878-3279</identifier><identifier>DOI: 10.1016/S0171-2985(85)80063-2</identifier><identifier>PMID: 2936676</identifier><identifier>CODEN: IMMND4</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>Animals ; Antibody Specificity ; Biological and medical sciences ; Catalase - pharmacology ; Complement C5 - immunology ; Complement C9 - immunology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Immunobiology ; Immunoglobulin G - immunology ; Luminescent Measurements ; Luminol ; Macrophages - immunology ; Male ; Mice ; Mice, Inbred Strains ; Myeloid cells: ontogeny, maturation, markers, receptors ; Opsonin Proteins ; Phagocytosis ; Receptors, Complement - immunology ; Receptors, Complement 3b ; Receptors, Fc - immunology ; Superoxide Dismutase - pharmacology</subject><ispartof>Immunobiology (1979), 1985-12, Vol.170 (5), p.390-401</ispartof><rights>1985 Gustav Fischer Verlag · Stuttgart · New York</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-fd64ce812e670465bd2e2c93320468feb600a8597df1a98015bfbe50a122f3403</citedby><cites>FETCH-LOGICAL-c389t-fd64ce812e670465bd2e2c93320468feb600a8597df1a98015bfbe50a122f3403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0171298585800632$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8814237$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2936676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Büscher, K.-H.</creatorcontrib><creatorcontrib>Klimetzek, V.</creatorcontrib><creatorcontrib>Opferkuch, W.</creatorcontrib><title>Influence of antibody and complement components on phagocytosis and chemiluminescence of macrophages</title><title>Immunobiology (1979)</title><addtitle>Immunobiology</addtitle><description>Macrophages are known to release reactive oxygen species (O
2
−,
1O
2, H
2O
2, OH·) in response to various membrane stimuliHowever, our studies show that phagocytic stimulation of macrophages is not necessarily accompanied by a stimulation of the oxidative burstWhereas IgG-opsonized erythrocytes were capable to induce phagocytosis and a chemiluminescence response, both being dependent on the number of IgG bound per erythrocyte, C3b-bearing erythrocytes were well ingested but failed to induce any chemiluminescence reactionFurthermore, stimulation of macrophages, via the Fc-receptors, seems to alter their functional state in regard to the activation of a receptor, which enables them to recognize membrane lesions on the target erythrocyteThe presence of IgG and membrane lesions, e.gthe C5b-9-complex of complement, induced a marked increase in chemiluminescence compared with stimulation by IgG-bearing particles aloneThe augmented response of macrophages was at least in part due to an additional release of H
2O
2, which was not liberated in response to IgG-bearing erythrocytesThis «Alesion recognizing receptor» in the macrophage membrane could not be activated by stimulation of C3b-receptors, indicating its functional linkage to the Fc-receptors.</description><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Catalase - pharmacology</subject><subject>Complement C5 - immunology</subject><subject>Complement C9 - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Immunobiology</subject><subject>Immunoglobulin G - immunology</subject><subject>Luminescent Measurements</subject><subject>Luminol</subject><subject>Macrophages - immunology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Myeloid cells: ontogeny, maturation, markers, receptors</subject><subject>Opsonin Proteins</subject><subject>Phagocytosis</subject><subject>Receptors, Complement - immunology</subject><subject>Receptors, Complement 3b</subject><subject>Receptors, Fc - immunology</subject><subject>Superoxide Dismutase - pharmacology</subject><issn>0171-2985</issn><issn>1878-3279</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVpSDbb_oSAD6WkByf6sPVxCiWkSWAhh7RnIUujRsGWNpZd2H9fObvda0GgEfPM6OVB6ILgK4IJv37GRJCaKtleyvabxJizmn5AKyKFrBkV6iNaHZEzdJ7zK8ZEUSFP0SlVjHPBV8g9Rt_PEC1UyVcmTqFLblcKV9k0bHsYIE7vZYqlylWK1fbF_E52N6Uc8p58gSH08xAiZPtv12DsmBYU8id04k2f4fPhXqNfP-5-3j7Um6f7x9vvm9oyqabaO95YkIQCF7jhbecoUKsYo-UlPXQcYyNbJZwnRklM2s530GJDKPWswWyNvu73bsf0NkOe9BBKoL43EdKcteCtYk3TFLDdgyViziN4vR3DYMadJlgvdvW7Xb2o08tZ7Gpa5i4OH8zdAO44ddBZ-l8OfZOt6f1oog35iElJGspEwW72GBQZfwKMOtuweHNhBDtpl8J_gvwF3uWX2Q</recordid><startdate>19851201</startdate><enddate>19851201</enddate><creator>Büscher, K.-H.</creator><creator>Klimetzek, V.</creator><creator>Opferkuch, W.</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19851201</creationdate><title>Influence of antibody and complement components on phagocytosis and chemiluminescence of macrophages</title><author>Büscher, K.-H. ; Klimetzek, V. ; Opferkuch, W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-fd64ce812e670465bd2e2c93320468feb600a8597df1a98015bfbe50a122f3403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Catalase - pharmacology</topic><topic>Complement C5 - immunology</topic><topic>Complement C9 - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Immunobiology</topic><topic>Immunoglobulin G - immunology</topic><topic>Luminescent Measurements</topic><topic>Luminol</topic><topic>Macrophages - immunology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Myeloid cells: ontogeny, maturation, markers, receptors</topic><topic>Opsonin Proteins</topic><topic>Phagocytosis</topic><topic>Receptors, Complement - immunology</topic><topic>Receptors, Complement 3b</topic><topic>Receptors, Fc - immunology</topic><topic>Superoxide Dismutase - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Büscher, K.-H.</creatorcontrib><creatorcontrib>Klimetzek, V.</creatorcontrib><creatorcontrib>Opferkuch, W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Immunobiology (1979)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Büscher, K.-H.</au><au>Klimetzek, V.</au><au>Opferkuch, W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of antibody and complement components on phagocytosis and chemiluminescence of macrophages</atitle><jtitle>Immunobiology (1979)</jtitle><addtitle>Immunobiology</addtitle><date>1985-12-01</date><risdate>1985</risdate><volume>170</volume><issue>5</issue><spage>390</spage><epage>401</epage><pages>390-401</pages><issn>0171-2985</issn><eissn>1878-3279</eissn><coden>IMMND4</coden><abstract>Macrophages are known to release reactive oxygen species (O
2
−,
1O
2, H
2O
2, OH·) in response to various membrane stimuliHowever, our studies show that phagocytic stimulation of macrophages is not necessarily accompanied by a stimulation of the oxidative burstWhereas IgG-opsonized erythrocytes were capable to induce phagocytosis and a chemiluminescence response, both being dependent on the number of IgG bound per erythrocyte, C3b-bearing erythrocytes were well ingested but failed to induce any chemiluminescence reactionFurthermore, stimulation of macrophages, via the Fc-receptors, seems to alter their functional state in regard to the activation of a receptor, which enables them to recognize membrane lesions on the target erythrocyteThe presence of IgG and membrane lesions, e.gthe C5b-9-complex of complement, induced a marked increase in chemiluminescence compared with stimulation by IgG-bearing particles aloneThe augmented response of macrophages was at least in part due to an additional release of H
2O
2, which was not liberated in response to IgG-bearing erythrocytesThis «Alesion recognizing receptor» in the macrophage membrane could not be activated by stimulation of C3b-receptors, indicating its functional linkage to the Fc-receptors.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><pmid>2936676</pmid><doi>10.1016/S0171-2985(85)80063-2</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0171-2985 |
| ispartof | Immunobiology (1979), 1985-12, Vol.170 (5), p.390-401 |
| issn | 0171-2985 1878-3279 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76593444 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibody Specificity Biological and medical sciences Catalase - pharmacology Complement C5 - immunology Complement C9 - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology Immunobiology Immunoglobulin G - immunology Luminescent Measurements Luminol Macrophages - immunology Male Mice Mice, Inbred Strains Myeloid cells: ontogeny, maturation, markers, receptors Opsonin Proteins Phagocytosis Receptors, Complement - immunology Receptors, Complement 3b Receptors, Fc - immunology Superoxide Dismutase - pharmacology |
| title | Influence of antibody and complement components on phagocytosis and chemiluminescence of macrophages |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T07%3A04%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Influence%20of%20antibody%20and%20complement%20components%20on%20phagocytosis%20and%20chemiluminescence%20of%20macrophages&rft.jtitle=Immunobiology%20(1979)&rft.au=B%C3%BCscher,%20K.-H.&rft.date=1985-12-01&rft.volume=170&rft.issue=5&rft.spage=390&rft.epage=401&rft.pages=390-401&rft.issn=0171-2985&rft.eissn=1878-3279&rft.coden=IMMND4&rft_id=info:doi/10.1016/S0171-2985(85)80063-2&rft_dat=%3Cproquest_cross%3E76593444%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76593444&rft_id=info:pmid/2936676&rft_els_id=S0171298585800632&rfr_iscdi=true |