Wild-type but not mutant APC associates with the microtubule cytoskeleton
The adenomatous polyposis coli protein (APC) is mutated in familial adenomatous polyposis patients as well as in sporadic colorectal tumors. In an attempt to further understand the function of APC, the subcellular localization of APC was examined. Wild-type and mutant forms of APC were expressed in...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1994-07, Vol.54 (14), p.3672-3675 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | SMITH, K. J LEVY, D. B MAUPIN, P POLLARD, T. D VOGELSTEIN, B KINZLER, K. W |
| description | The adenomatous polyposis coli protein (APC) is mutated in familial adenomatous polyposis patients as well as in sporadic colorectal tumors. In an attempt to further understand the function of APC, the subcellular localization of APC was examined. Wild-type and mutant forms of APC were expressed in mammalian cells and protein detected by immunofluorescence using monoclonal and polyclonal antibodies. Staining of wildtype APC protein revealed a filamentous network which extended throughout the cytoplasm and colocalized with microtubules. In striking contrast, mutant APC protein gave a diffuse cytoplasmic staining pattern. Treatment with a microtubule depolymerizing agent, nocodazole, caused APC as well as tubulin to become diffusely cytoplasmic. In addition, immunoperoxidase staining of transfected APC protein followed by transmission electron microscopy revealed staining of microtubules. These results suggest that wild-type but not mutant APC protein may be associated with the microtubule cytoskeleton. |
| format | Article |
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J ; LEVY, D. B ; MAUPIN, P ; POLLARD, T. D ; VOGELSTEIN, B ; KINZLER, K. W</creator><creatorcontrib>SMITH, K. J ; LEVY, D. B ; MAUPIN, P ; POLLARD, T. D ; VOGELSTEIN, B ; KINZLER, K. W</creatorcontrib><description>The adenomatous polyposis coli protein (APC) is mutated in familial adenomatous polyposis patients as well as in sporadic colorectal tumors. In an attempt to further understand the function of APC, the subcellular localization of APC was examined. Wild-type and mutant forms of APC were expressed in mammalian cells and protein detected by immunofluorescence using monoclonal and polyclonal antibodies. Staining of wildtype APC protein revealed a filamentous network which extended throughout the cytoplasm and colocalized with microtubules. In striking contrast, mutant APC protein gave a diffuse cytoplasmic staining pattern. Treatment with a microtubule depolymerizing agent, nocodazole, caused APC as well as tubulin to become diffusely cytoplasmic. In addition, immunoperoxidase staining of transfected APC protein followed by transmission electron microscopy revealed staining of microtubules. These results suggest that wild-type but not mutant APC protein may be associated with the microtubule cytoskeleton.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 8033082</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>3T3 Cells ; Adenomatous Polyposis Coli Protein ; Animals ; Biological and medical sciences ; Cytoskeletal Proteins - analysis ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Medical sciences ; Mice ; Microtubule Proteins - analysis ; Microtubules - chemistry ; Mutation ; Stomach. Duodenum. Small intestine. Colon. Rectum. 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W</creatorcontrib><title>Wild-type but not mutant APC associates with the microtubule cytoskeleton</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>The adenomatous polyposis coli protein (APC) is mutated in familial adenomatous polyposis patients as well as in sporadic colorectal tumors. In an attempt to further understand the function of APC, the subcellular localization of APC was examined. Wild-type and mutant forms of APC were expressed in mammalian cells and protein detected by immunofluorescence using monoclonal and polyclonal antibodies. Staining of wildtype APC protein revealed a filamentous network which extended throughout the cytoplasm and colocalized with microtubules. In striking contrast, mutant APC protein gave a diffuse cytoplasmic staining pattern. Treatment with a microtubule depolymerizing agent, nocodazole, caused APC as well as tubulin to become diffusely cytoplasmic. In addition, immunoperoxidase staining of transfected APC protein followed by transmission electron microscopy revealed staining of microtubules. These results suggest that wild-type but not mutant APC protein may be associated with the microtubule cytoskeleton.</description><subject>3T3 Cells</subject><subject>Adenomatous Polyposis Coli Protein</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cytoskeletal Proteins - analysis</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Microtubule Proteins - analysis</subject><subject>Microtubules - chemistry</subject><subject>Mutation</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. 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B</creatorcontrib><creatorcontrib>MAUPIN, P</creatorcontrib><creatorcontrib>POLLARD, T. D</creatorcontrib><creatorcontrib>VOGELSTEIN, B</creatorcontrib><creatorcontrib>KINZLER, K. W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SMITH, K. J</au><au>LEVY, D. B</au><au>MAUPIN, P</au><au>POLLARD, T. D</au><au>VOGELSTEIN, B</au><au>KINZLER, K. 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Staining of wildtype APC protein revealed a filamentous network which extended throughout the cytoplasm and colocalized with microtubules. In striking contrast, mutant APC protein gave a diffuse cytoplasmic staining pattern. Treatment with a microtubule depolymerizing agent, nocodazole, caused APC as well as tubulin to become diffusely cytoplasmic. In addition, immunoperoxidase staining of transfected APC protein followed by transmission electron microscopy revealed staining of microtubules. These results suggest that wild-type but not mutant APC protein may be associated with the microtubule cytoskeleton.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>8033082</pmid><tpages>4</tpages></addata></record> |
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| ispartof | Cancer research (Chicago, Ill.), 1994-07, Vol.54 (14), p.3672-3675 |
| issn | 0008-5472 1538-7445 |
| language | eng |
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| source | MEDLINE; American Association for Cancer Research; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 3T3 Cells Adenomatous Polyposis Coli Protein Animals Biological and medical sciences Cytoskeletal Proteins - analysis Gastroenterology. Liver. Pancreas. Abdomen Humans Medical sciences Mice Microtubule Proteins - analysis Microtubules - chemistry Mutation Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tumors |
| title | Wild-type but not mutant APC associates with the microtubule cytoskeleton |
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