Determination of the HNK-1 epitope (3-sulphated glucuronic acid) in intact chondroitin sulphates by ELISA. Application to squid skin proteoglycans and their oversulphated carbohydrate structures
The reactivity of the HNK-1 monoclonal antibody to chondroitin sulphates and derived disaccharides was studied using an ELISA inhibition test. The antibody readily reacted with its specific epitope (3-sulphated glucuronic acid) in intact chondroitin sulphates as well as with the equivalent oversulph...
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| Veröffentlicht in: | Biochimie 1994, Vol.76 (1), p.79-82 |
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| creator | Karamanos, N.K. Aletras, A.J. Antonopoulos, C.A. Hjerpe, A. |
| description | The reactivity of the HNK-1 monoclonal antibody to chondroitin sulphates and derived disaccharides was studied using an ELISA inhibition test. The antibody readily reacted with its specific epitope (3-sulphated glucuronic acid) in intact chondroitin sulphates as well as with the equivalent oversulphated
Δ
4-disaccharides obtained by chondroitinase digestion and identified as sulphated at C-3 of the hexuronate. It is showed that by using the oversulphated
Δ
4-disaccharides as standards in an ELISA inhibition test, the amount of 3-sulphated glucuronic acid can be estimated also in the polymer preparations. When applying this ELISA test to the PG populations isolated from squid skin, most of the oversulphation seen in HPLC analyses of these preparations was found to be associated with 3-sulphation of the glucuronic acid. |
| doi_str_mv | 10.1016/0300-9084(94)90066-3 |
| format | Article |
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Δ
4-disaccharides obtained by chondroitinase digestion and identified as sulphated at C-3 of the hexuronate. It is showed that by using the oversulphated
Δ
4-disaccharides as standards in an ELISA inhibition test, the amount of 3-sulphated glucuronic acid can be estimated also in the polymer preparations. When applying this ELISA test to the PG populations isolated from squid skin, most of the oversulphation seen in HPLC analyses of these preparations was found to be associated with 3-sulphation of the glucuronic acid.</description><identifier>ISSN: 0300-9084</identifier><identifier>EISSN: 1638-6183</identifier><identifier>DOI: 10.1016/0300-9084(94)90066-3</identifier><identifier>PMID: 7518256</identifier><identifier>CODEN: BICMBE</identifier><language>eng</language><publisher>Paris: Elsevier Masson SAS</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Antibodies, Monoclonal - immunology ; Antibody Specificity ; Biological and medical sciences ; Carbohydrates ; Chondroitin Sulfates - chemistry ; Chondroitin Sulfates - immunology ; chondroitin sulphates ; Chondroitinases and Chondroitin Lyases - metabolism ; Chromatography, High Pressure Liquid ; Decapodiformes ; Disaccharides - analysis ; Disaccharides - metabolism ; Enzyme-Linked Immunosorbent Assay ; Epitopes ; Fundamental and applied biological sciences. Psychology ; Glucuronates - analysis ; Glucuronates - immunology ; Glucuronic Acid ; HNK-1 monoclonal antibody ; Holosides ; Marine ; Other biological molecules ; proteoglycans ; Skin - chemistry ; Skin - immunology ; squid skin</subject><ispartof>Biochimie, 1994, Vol.76 (1), p.79-82</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-426edbebbda3f525f44ac069c825ba53897266ee019c0dd1a4e9931b38987f273</citedby><cites>FETCH-LOGICAL-c417t-426edbebbda3f525f44ac069c825ba53897266ee019c0dd1a4e9931b38987f273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0300-9084(94)90066-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,4010,27904,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4126332$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7518256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karamanos, N.K.</creatorcontrib><creatorcontrib>Aletras, A.J.</creatorcontrib><creatorcontrib>Antonopoulos, C.A.</creatorcontrib><creatorcontrib>Hjerpe, A.</creatorcontrib><title>Determination of the HNK-1 epitope (3-sulphated glucuronic acid) in intact chondroitin sulphates by ELISA. Application to squid skin proteoglycans and their oversulphated carbohydrate structures</title><title>Biochimie</title><addtitle>Biochimie</addtitle><description>The reactivity of the HNK-1 monoclonal antibody to chondroitin sulphates and derived disaccharides was studied using an ELISA inhibition test. The antibody readily reacted with its specific epitope (3-sulphated glucuronic acid) in intact chondroitin sulphates as well as with the equivalent oversulphated
Δ
4-disaccharides obtained by chondroitinase digestion and identified as sulphated at C-3 of the hexuronate. It is showed that by using the oversulphated
Δ
4-disaccharides as standards in an ELISA inhibition test, the amount of 3-sulphated glucuronic acid can be estimated also in the polymer preparations. When applying this ELISA test to the PG populations isolated from squid skin, most of the oversulphation seen in HPLC analyses of these preparations was found to be associated with 3-sulphation of the glucuronic acid.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Carbohydrates</subject><subject>Chondroitin Sulfates - chemistry</subject><subject>Chondroitin Sulfates - immunology</subject><subject>chondroitin sulphates</subject><subject>Chondroitinases and Chondroitin Lyases - metabolism</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Decapodiformes</subject><subject>Disaccharides - analysis</subject><subject>Disaccharides - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucuronates - analysis</subject><subject>Glucuronates - immunology</subject><subject>Glucuronic Acid</subject><subject>HNK-1 monoclonal antibody</subject><subject>Holosides</subject><subject>Marine</subject><subject>Other biological molecules</subject><subject>proteoglycans</subject><subject>Skin - chemistry</subject><subject>Skin - immunology</subject><subject>squid skin</subject><issn>0300-9084</issn><issn>1638-6183</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EKkvhDUDyAaH2kGLHjhNfkFal0IoVHICz5diTriFrp7ZTaV-vT1avdlluII1keeabmV_zI_SakgtKqHhPGCGVJB0_k_xcEiJExZ6gBRWsqwTt2FO0OCLP0YuUfhFCGlLLE3TSNrSrG7FADx8hQ9w4r7MLHocB5zXg669fKophcjlMgM9YleZxWusMFt-Os5lj8M5gbZw9x86XyNpkbNbB2xhcLqk_DQn3W3y1uvm-vMDLaRqd2S_KAae72Vmcfhd6iiFDuB23RvuEtbc7FS7icA_x72qjYx_WWxvLD6ccZ5PnCOklejboMcGrw3uKfn66-nF5Xa2-fb65XK4qw2mbK14LsD30vdVsaOpm4FwbIqQph-h1wzrZ1kIAECoNsZZqDlIy2pdC1w51y07Ru_3covZuhpTVxiUD46g9hDmpVjQF7eh_QSo6wmvaFJDvQRNDShEGNUW30XGrKFE7j9XOQLUzUMkSO48VK21vDvPnfgP22HQwtdTfHuo6GT0OUXvj0hHjtBaM1QX7sMegHO3eQVTJOPAGrItgsrLB_VvHI-D9xl4</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Karamanos, N.K.</creator><creator>Aletras, A.J.</creator><creator>Antonopoulos, C.A.</creator><creator>Hjerpe, A.</creator><general>Elsevier Masson SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>Determination of the HNK-1 epitope (3-sulphated glucuronic acid) in intact chondroitin sulphates by ELISA. Application to squid skin proteoglycans and their oversulphated carbohydrate structures</title><author>Karamanos, N.K. ; Aletras, A.J. ; Antonopoulos, C.A. ; Hjerpe, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-426edbebbda3f525f44ac069c825ba53897266ee019c0dd1a4e9931b38987f273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Carbohydrates</topic><topic>Chondroitin Sulfates - chemistry</topic><topic>Chondroitin Sulfates - immunology</topic><topic>chondroitin sulphates</topic><topic>Chondroitinases and Chondroitin Lyases - metabolism</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Decapodiformes</topic><topic>Disaccharides - analysis</topic><topic>Disaccharides - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucuronates - analysis</topic><topic>Glucuronates - immunology</topic><topic>Glucuronic Acid</topic><topic>HNK-1 monoclonal antibody</topic><topic>Holosides</topic><topic>Marine</topic><topic>Other biological molecules</topic><topic>proteoglycans</topic><topic>Skin - chemistry</topic><topic>Skin - immunology</topic><topic>squid skin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karamanos, N.K.</creatorcontrib><creatorcontrib>Aletras, A.J.</creatorcontrib><creatorcontrib>Antonopoulos, C.A.</creatorcontrib><creatorcontrib>Hjerpe, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Biochimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karamanos, N.K.</au><au>Aletras, A.J.</au><au>Antonopoulos, C.A.</au><au>Hjerpe, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of the HNK-1 epitope (3-sulphated glucuronic acid) in intact chondroitin sulphates by ELISA. Application to squid skin proteoglycans and their oversulphated carbohydrate structures</atitle><jtitle>Biochimie</jtitle><addtitle>Biochimie</addtitle><date>1994</date><risdate>1994</risdate><volume>76</volume><issue>1</issue><spage>79</spage><epage>82</epage><pages>79-82</pages><issn>0300-9084</issn><eissn>1638-6183</eissn><coden>BICMBE</coden><abstract>The reactivity of the HNK-1 monoclonal antibody to chondroitin sulphates and derived disaccharides was studied using an ELISA inhibition test. The antibody readily reacted with its specific epitope (3-sulphated glucuronic acid) in intact chondroitin sulphates as well as with the equivalent oversulphated
Δ
4-disaccharides obtained by chondroitinase digestion and identified as sulphated at C-3 of the hexuronate. It is showed that by using the oversulphated
Δ
4-disaccharides as standards in an ELISA inhibition test, the amount of 3-sulphated glucuronic acid can be estimated also in the polymer preparations. When applying this ELISA test to the PG populations isolated from squid skin, most of the oversulphation seen in HPLC analyses of these preparations was found to be associated with 3-sulphation of the glucuronic acid.</abstract><cop>Paris</cop><pub>Elsevier Masson SAS</pub><pmid>7518256</pmid><doi>10.1016/0300-9084(94)90066-3</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biochimie, 1994, Vol.76 (1), p.79-82 |
| issn | 0300-9084 1638-6183 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical, structural and metabolic biochemistry Animals Antibodies, Monoclonal - immunology Antibody Specificity Biological and medical sciences Carbohydrates Chondroitin Sulfates - chemistry Chondroitin Sulfates - immunology chondroitin sulphates Chondroitinases and Chondroitin Lyases - metabolism Chromatography, High Pressure Liquid Decapodiformes Disaccharides - analysis Disaccharides - metabolism Enzyme-Linked Immunosorbent Assay Epitopes Fundamental and applied biological sciences. Psychology Glucuronates - analysis Glucuronates - immunology Glucuronic Acid HNK-1 monoclonal antibody Holosides Marine Other biological molecules proteoglycans Skin - chemistry Skin - immunology squid skin |
| title | Determination of the HNK-1 epitope (3-sulphated glucuronic acid) in intact chondroitin sulphates by ELISA. Application to squid skin proteoglycans and their oversulphated carbohydrate structures |
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