Sequence and expression of a baculovirus protein with antigenic similarity to telokin
A protein from baculovirus-infected cells reacted with an antibody against the smooth muscle protein telokin. Because of this unusual similarity, the protein, termed telokin-like protein-20 (TLP20), was isolated and characterized. Its Mr on denaturing polyacrylamide gels was 28K and the protein cont...
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| Veröffentlicht in: | Journal of general virology 1994-07, Vol.75 (7), p.1807-1809 |
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| creator | Raynes, D.A Hartshorne, D.J Guerriero, V. Jr |
| description | A protein from baculovirus-infected cells reacted with an antibody against the smooth muscle protein telokin. Because of this unusual similarity, the protein, termed telokin-like protein-20 (TLP20), was isolated and characterized. Its Mr on denaturing polyacrylamide gels was 28K and the protein contained a high proportion of beta structure. A cDNA for TLP20 was isolated and sequenced. The 3' non-coding sequence contained a region of high identity with the 5' end of two other baculovirus genes. The 5' non-coding region contains several baculovirus regulatory elements. Surprisingly, the derived amino acid sequence showed no homologies to telokin. The cDNA was cloned into a bacterial expression vector and the subsequently expressed protein had a slightly lower Mr than the native protein, but cross-reacted with telokin antibody. This paper reports the characterization of a new baculovirus protein that shares some antigenic similarities to the smooth muscle protein telokin. |
| doi_str_mv | 10.1099/0022-1317-75-7-1807 |
| format | Article |
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This paper reports the characterization of a new baculovirus protein that shares some antigenic similarities to the smooth muscle protein telokin.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-75-7-1807</identifier><identifier>PMID: 7517434</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Antibodies ; antigens ; Autographa californica ; Baculoviridae ; baculovirus ; Base Sequence ; Biological and medical sciences ; cell culture ; Cross Reactions ; DNA, Complementary ; Epitopes - analysis ; Fundamental and applied biological sciences. 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Jr</creatorcontrib><title>Sequence and expression of a baculovirus protein with antigenic similarity to telokin</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>A protein from baculovirus-infected cells reacted with an antibody against the smooth muscle protein telokin. Because of this unusual similarity, the protein, termed telokin-like protein-20 (TLP20), was isolated and characterized. Its Mr on denaturing polyacrylamide gels was 28K and the protein contained a high proportion of beta structure. A cDNA for TLP20 was isolated and sequenced. The 3' non-coding sequence contained a region of high identity with the 5' end of two other baculovirus genes. The 5' non-coding region contains several baculovirus regulatory elements. Surprisingly, the derived amino acid sequence showed no homologies to telokin. The cDNA was cloned into a bacterial expression vector and the subsequently expressed protein had a slightly lower Mr than the native protein, but cross-reacted with telokin antibody. This paper reports the characterization of a new baculovirus protein that shares some antigenic similarities to the smooth muscle protein telokin.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Antibodies</subject><subject>antigens</subject><subject>Autographa californica</subject><subject>Baculoviridae</subject><subject>baculovirus</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cell culture</subject><subject>Cross Reactions</subject><subject>DNA, Complementary</subject><subject>Epitopes - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genbank/u06931</subject><subject>Genes, Viral - genetics</subject><subject>Isoelectric Point</subject><subject>Manduca sexta</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Muscle Proteins - chemistry</subject><subject>Muscle Proteins - genetics</subject><subject>Muscle Proteins - immunology</subject><subject>Myosin-Light-Chain Kinase</subject><subject>Nucleopolyhedrovirus</subject><subject>Nucleopolyhedrovirus - chemistry</subject><subject>Nucleopolyhedrovirus - genetics</subject><subject>nucleotide sequences</subject><subject>Open Reading Frames</subject><subject>Peptide Fragments</subject><subject>Peptides</subject><subject>Protein Structure, Secondary</subject><subject>proteins</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Viral Proteins - chemistry</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - immunology</subject><subject>Viral Structural Proteins - genetics</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1P3DAQhq0KBNttf0FV4UNVcUnrr3iSI0LQIiH1QPdsOc5k120SL3ZS4N_Xq12tuHGaw_vMePwMIZ84-8ZZXX9nTIiCSw4FlAUUvGLwjiy40mUhcn5CFkfinLxP6Q9jXKkSzsgZlByUVAuyesDHGUeH1I4txedtxJR8GGnoqKWNdXMf_vk4J7qNYUI_0ic_bTI8-TWO3tHkB9_b6KcXOgU6YR_--vEDOe1sn_DjoS7J6vbm9_XP4v7Xj7vrq_vCKS2mAkCBbkpkQsoakZWNcpVyrIVGMN5Aq1jFtMVcoZaWa97KJnOqtrVznZRL8nU_Ny-Xv5EmM_jksO_tiGFOBnRZ1ULCmyDXWqiKqwzKPehiSCliZ7bRDza-GM7MzrrZOTU7pwZKA2ZnPXd9PoyfmwHbY89Bc86_HHKbnO27aEfn0xFTHITMey7J5R7b-PXmyUc02fHg8yqNDyaf4fWLF3u0s8HYdczTVg9ZmswnriXXUv4HrQShRw</recordid><startdate>19940701</startdate><enddate>19940701</enddate><creator>Raynes, D.A</creator><creator>Hartshorne, D.J</creator><creator>Guerriero, V. Jr</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19940701</creationdate><title>Sequence and expression of a baculovirus protein with antigenic similarity to telokin</title><author>Raynes, D.A ; Hartshorne, D.J ; Guerriero, V. Jr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-77476b5e02339ee05b4c84c0d7b201b7d40806aed40793a161d3bee049a9ccf33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Antibodies</topic><topic>antigens</topic><topic>Autographa californica</topic><topic>Baculoviridae</topic><topic>baculovirus</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cell culture</topic><topic>Cross Reactions</topic><topic>DNA, Complementary</topic><topic>Epitopes - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genbank/u06931</topic><topic>Genes, Viral - genetics</topic><topic>Isoelectric Point</topic><topic>Manduca sexta</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Muscle Proteins - chemistry</topic><topic>Muscle Proteins - genetics</topic><topic>Muscle Proteins - immunology</topic><topic>Myosin-Light-Chain Kinase</topic><topic>Nucleopolyhedrovirus</topic><topic>Nucleopolyhedrovirus - chemistry</topic><topic>Nucleopolyhedrovirus - genetics</topic><topic>nucleotide sequences</topic><topic>Open Reading Frames</topic><topic>Peptide Fragments</topic><topic>Peptides</topic><topic>Protein Structure, Secondary</topic><topic>proteins</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Viral Proteins - chemistry</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - immunology</topic><topic>Viral Structural Proteins - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raynes, D.A</creatorcontrib><creatorcontrib>Hartshorne, D.J</creatorcontrib><creatorcontrib>Guerriero, V. 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Jr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence and expression of a baculovirus protein with antigenic similarity to telokin</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1994-07-01</date><risdate>1994</risdate><volume>75</volume><issue>7</issue><spage>1807</spage><epage>1809</epage><pages>1807-1809</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>A protein from baculovirus-infected cells reacted with an antibody against the smooth muscle protein telokin. Because of this unusual similarity, the protein, termed telokin-like protein-20 (TLP20), was isolated and characterized. Its Mr on denaturing polyacrylamide gels was 28K and the protein contained a high proportion of beta structure. A cDNA for TLP20 was isolated and sequenced. The 3' non-coding sequence contained a region of high identity with the 5' end of two other baculovirus genes. The 5' non-coding region contains several baculovirus regulatory elements. Surprisingly, the derived amino acid sequence showed no homologies to telokin. The cDNA was cloned into a bacterial expression vector and the subsequently expressed protein had a slightly lower Mr than the native protein, but cross-reacted with telokin antibody. This paper reports the characterization of a new baculovirus protein that shares some antigenic similarities to the smooth muscle protein telokin.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>7517434</pmid><doi>10.1099/0022-1317-75-7-1807</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1994-07, Vol.75 (7), p.1807-1809 |
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| source | MEDLINE; Microbiology Society; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Amino Acid Sequence amino acid sequences Antibodies antigens Autographa californica Baculoviridae baculovirus Base Sequence Biological and medical sciences cell culture Cross Reactions DNA, Complementary Epitopes - analysis Fundamental and applied biological sciences. Psychology genbank/u06931 Genes, Viral - genetics Isoelectric Point Manduca sexta Microbiology Molecular Sequence Data Molecular Weight Muscle Proteins - chemistry Muscle Proteins - genetics Muscle Proteins - immunology Myosin-Light-Chain Kinase Nucleopolyhedrovirus Nucleopolyhedrovirus - chemistry Nucleopolyhedrovirus - genetics nucleotide sequences Open Reading Frames Peptide Fragments Peptides Protein Structure, Secondary proteins Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Sequence Analysis, DNA Sequence Homology, Amino Acid Viral Proteins - chemistry Viral Proteins - genetics Viral Proteins - immunology Viral Structural Proteins - genetics Virology |
| title | Sequence and expression of a baculovirus protein with antigenic similarity to telokin |
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