Expression of 72-kDa Gelatinase and TIMP-2 in Early and Late Passage Human Fibroblasts

The expression and regulation of 72-kDa gelatinase and TIMP-2 were examined in cultures of early and late passage human fibroblasts. In contrast to collagenase expression, which was low in early passage cells and highly expressed in late passage fibroblasts, 72-kDa gelatinase mRNA expression was enh...

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Veröffentlicht in:	Experimental cell research 1994-07, Vol.213 (1), p.148-155
Hauptverfasser:	Zeng, Guangyuan, Millis, Albert J.T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal Base Sequence Biological and medical sciences Blotting, Northern Cellular Senescence Chromatography, Affinity DNA Primers Electrophoresis, Polyacrylamide Gel Fibroblasts - cytology Fibroblasts - metabolism Fundamental and applied biological sciences. Psychology Gelatinases - biosynthesis Gelatinases - isolation & purification Gene Expression Gene Expression Regulation Humans Metalloendopeptidases - antagonists & inhibitors Molecular and cellular biology Molecular genetics Molecular Sequence Data Molecular Weight Polymerase Chain Reaction Protein Biosynthesis Proteins - isolation & purification RNA, Messenger - analysis RNA, Messenger - biosynthesis Tissue Inhibitor of Metalloproteinase-2
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