Comparative development of Cryptosporidium parvum (Apicomplexa) in 11 continuous host cell lines
Using standardized media, incubation, and parasite inoculating procedures, we compared development of Cryptosporidium parvum between Madin-Darby bovine kidney (MDBK) cells and 10 additional host cell lines available through the American Type Culture Collection. Parasite development was assessed by c...
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| Veröffentlicht in: | FEMS microbiology letters 1994-05, Vol.118 (3), p.233-236 |
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| container_title | FEMS microbiology letters |
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| creator | UPTON, S. J TILLEY, M BRILLHART, D. B |
| description | Using standardized media, incubation, and parasite inoculating procedures, we compared development of Cryptosporidium parvum between Madin-Darby bovine kidney (MDBK) cells and 10 additional host cell lines available through the American Type Culture Collection. Parasite development was assessed by counting parasite numbers atop monolayers in 25 random oil fields 68 h post-infection using Nomarski interference-contrast optics. Results revealed that the human ileocecal adenocarcinoma (HCT-8) cell line supported nearly twice the number of parasite developmental stages than MDBK cells or any of the other host cell types. |
| doi_str_mv | 10.1016/0378-1097(94)90509-6 |
| format | Article |
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Results revealed that the human ileocecal adenocarcinoma (HCT-8) cell line supported nearly twice the number of parasite developmental stages than MDBK cells or any of the other host cell types.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/0378-1097(94)90509-6</identifier><identifier>PMID: 8020747</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; Cell Line ; Cells, Cultured ; Cryptosporidium parvum - growth & development ; Dogs ; Fundamental and applied biological sciences. Psychology ; Invertebrates ; Kidney - cytology ; Life cycle. Host-agent relationship. 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Results revealed that the human ileocecal adenocarcinoma (HCT-8) cell line supported nearly twice the number of parasite developmental stages than MDBK cells or any of the other host cell types.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Cryptosporidium parvum - growth & development</subject><subject>Dogs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Invertebrates</subject><subject>Kidney - cytology</subject><subject>Life cycle. Host-agent relationship. 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Psychology</topic><topic>Invertebrates</topic><topic>Kidney - cytology</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Mice</topic><topic>Protozoa</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>UPTON, S. J</creatorcontrib><creatorcontrib>TILLEY, M</creatorcontrib><creatorcontrib>BRILLHART, D. B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>UPTON, S. J</au><au>TILLEY, M</au><au>BRILLHART, D. 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| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1994-05, Vol.118 (3), p.233-236 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals Digital Archive Legacy; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Cattle Cell Line Cells, Cultured Cryptosporidium parvum - growth & development Dogs Fundamental and applied biological sciences. Psychology Invertebrates Kidney - cytology Life cycle. Host-agent relationship. Pathogenesis Mice Protozoa Species Specificity |
| title | Comparative development of Cryptosporidium parvum (Apicomplexa) in 11 continuous host cell lines |
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