D-Ribose and Deoxy-D-Ribose Induce Apoptosis in Human Quiescent Peripheral Blood Mononuclear Cells
In previous papers we reported that D(−)-ribose and 2-deoxy-D-ribose, which rank at the top among reducing sugars, kill a variety of human and animal cells and cell lines [1, 2]. Here we demonstrate that these two sugars induce apoptosis in human quiescent peripheral blood mononuclear cells which ar...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1994-06, Vol.201 (3), p.1109-1116 |
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| creator | Barbieri, D. Grassilli, E. Monti, D. Salvioli, S. Franceschini, M.G. Franchini, A. Bellesia, E. Salomoni, P. Negro, P. Capri, M. Troiano, L. Cossarizza, A. Franceschi, C. |
| description | In previous papers we reported that D(−)-ribose and 2-deoxy-D-ribose, which rank at the top among reducing sugars, kill a variety of human and animal cells and cell lines [1, 2]. Here we demonstrate that these two sugars induce apoptosis in human quiescent peripheral blood mononuclear cells which are relatively insensitive to apoptosis. Apoptosis was assessed by morphological changes, DNA fragmentation by agarose gel electrophoresis and the appearance of an hypodiploid peak by flow cytometry. 2-deoxy-D-ribose was more potent than D(−)-ribose and apoptosis was evident from 48 hours of culture onwards. 2-deoxy-D-ribose-induced apoptosis was inibited by N-acetyl-L-cysteine, suggesting that glutathione metabolism and/or oxidative stress are involved in this type of apoptosis. Thus, D(−)-ribose and 2-deoxy-D-ribose can be useful tools to study the cellular and molecular events of apoptosis in human quiescent lymphocytes. |
| doi_str_mv | 10.1006/bbrc.1994.1820 |
| format | Article |
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Here we demonstrate that these two sugars induce apoptosis in human quiescent peripheral blood mononuclear cells which are relatively insensitive to apoptosis. Apoptosis was assessed by morphological changes, DNA fragmentation by agarose gel electrophoresis and the appearance of an hypodiploid peak by flow cytometry. 2-deoxy-D-ribose was more potent than D(−)-ribose and apoptosis was evident from 48 hours of culture onwards. 2-deoxy-D-ribose-induced apoptosis was inibited by N-acetyl-L-cysteine, suggesting that glutathione metabolism and/or oxidative stress are involved in this type of apoptosis. Thus, D(−)-ribose and 2-deoxy-D-ribose can be useful tools to study the cellular and molecular events of apoptosis in human quiescent lymphocytes.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1994.1820</identifier><identifier>PMID: 8024552</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetylcysteine - pharmacology ; Apoptosis - drug effects ; Deoxyribose - pharmacology ; DNA Damage ; Humans ; Leukocytes, Mononuclear - cytology ; Leukocytes, Mononuclear - drug effects ; Ribose - pharmacology</subject><ispartof>Biochemical and biophysical research communications, 1994-06, Vol.201 (3), p.1109-1116</ispartof><rights>1994 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-6b567521a3686e209ee3a6480389bfeb275243461d40822a6e3c0f3820e0dbb23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1994.1820$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8024552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barbieri, D.</creatorcontrib><creatorcontrib>Grassilli, E.</creatorcontrib><creatorcontrib>Monti, D.</creatorcontrib><creatorcontrib>Salvioli, S.</creatorcontrib><creatorcontrib>Franceschini, M.G.</creatorcontrib><creatorcontrib>Franchini, A.</creatorcontrib><creatorcontrib>Bellesia, E.</creatorcontrib><creatorcontrib>Salomoni, P.</creatorcontrib><creatorcontrib>Negro, P.</creatorcontrib><creatorcontrib>Capri, M.</creatorcontrib><creatorcontrib>Troiano, L.</creatorcontrib><creatorcontrib>Cossarizza, A.</creatorcontrib><creatorcontrib>Franceschi, C.</creatorcontrib><title>D-Ribose and Deoxy-D-Ribose Induce Apoptosis in Human Quiescent Peripheral Blood Mononuclear Cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>In previous papers we reported that D(−)-ribose and 2-deoxy-D-ribose, which rank at the top among reducing sugars, kill a variety of human and animal cells and cell lines [1, 2]. Here we demonstrate that these two sugars induce apoptosis in human quiescent peripheral blood mononuclear cells which are relatively insensitive to apoptosis. Apoptosis was assessed by morphological changes, DNA fragmentation by agarose gel electrophoresis and the appearance of an hypodiploid peak by flow cytometry. 2-deoxy-D-ribose was more potent than D(−)-ribose and apoptosis was evident from 48 hours of culture onwards. 2-deoxy-D-ribose-induced apoptosis was inibited by N-acetyl-L-cysteine, suggesting that glutathione metabolism and/or oxidative stress are involved in this type of apoptosis. Thus, D(−)-ribose and 2-deoxy-D-ribose can be useful tools to study the cellular and molecular events of apoptosis in human quiescent lymphocytes.</description><subject>Acetylcysteine - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Deoxyribose - pharmacology</subject><subject>DNA Damage</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Ribose - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFr2zAUh8Xo6NJu190KOvXm9EmWZeuYJVtTyOg2WthNSPIL1XAkT7JL-9_XIaG30tMD_T79eO8j5CuDOQOQV9YmN2dKiTlrOHwgMwYKCs5AnJAZTETBFfv7iZzl_A-AMSHVKTltgIuq4jNiV8Ufb2NGakJLVxifnovXp5vQjg7poo_9ELPP1Ae6Hncm0N-jx-wwDPQXJt8_YDId_dbF2NKfMcQwug5NokvsuvyZfNyaLuOX4zwn9z--3y3Xxeb2-ma52BSurGEopK1kXXFmStlI5KAQSyNFA2Wj7BYtn0JRCslaAQ3nRmLpYFtORyO01vLynFweevsU_4-YB73z045dZwLGMetaVrUStXoXZLKuQVQwgfMD6FLMOeFW98nvTHrWDPTevt7b13v7em9_-nBxbB7tDttX_Kh7yptDjpOHR49JZ-cxOGx9QjfoNvq3ql8A772SHQ</recordid><startdate>19940630</startdate><enddate>19940630</enddate><creator>Barbieri, D.</creator><creator>Grassilli, E.</creator><creator>Monti, D.</creator><creator>Salvioli, S.</creator><creator>Franceschini, M.G.</creator><creator>Franchini, A.</creator><creator>Bellesia, E.</creator><creator>Salomoni, P.</creator><creator>Negro, P.</creator><creator>Capri, M.</creator><creator>Troiano, L.</creator><creator>Cossarizza, A.</creator><creator>Franceschi, C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19940630</creationdate><title>D-Ribose and Deoxy-D-Ribose Induce Apoptosis in Human Quiescent Peripheral Blood Mononuclear Cells</title><author>Barbieri, D. ; Grassilli, E. ; Monti, D. ; Salvioli, S. ; Franceschini, M.G. ; Franchini, A. ; Bellesia, E. ; Salomoni, P. ; Negro, P. ; Capri, M. ; Troiano, L. ; Cossarizza, A. ; Franceschi, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-6b567521a3686e209ee3a6480389bfeb275243461d40822a6e3c0f3820e0dbb23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Acetylcysteine - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Deoxyribose - pharmacology</topic><topic>DNA Damage</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - cytology</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Ribose - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barbieri, D.</creatorcontrib><creatorcontrib>Grassilli, E.</creatorcontrib><creatorcontrib>Monti, D.</creatorcontrib><creatorcontrib>Salvioli, S.</creatorcontrib><creatorcontrib>Franceschini, M.G.</creatorcontrib><creatorcontrib>Franchini, A.</creatorcontrib><creatorcontrib>Bellesia, E.</creatorcontrib><creatorcontrib>Salomoni, P.</creatorcontrib><creatorcontrib>Negro, P.</creatorcontrib><creatorcontrib>Capri, M.</creatorcontrib><creatorcontrib>Troiano, L.</creatorcontrib><creatorcontrib>Cossarizza, A.</creatorcontrib><creatorcontrib>Franceschi, C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barbieri, D.</au><au>Grassilli, E.</au><au>Monti, D.</au><au>Salvioli, S.</au><au>Franceschini, M.G.</au><au>Franchini, A.</au><au>Bellesia, E.</au><au>Salomoni, P.</au><au>Negro, P.</au><au>Capri, M.</au><au>Troiano, L.</au><au>Cossarizza, A.</au><au>Franceschi, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>D-Ribose and Deoxy-D-Ribose Induce Apoptosis in Human Quiescent Peripheral Blood Mononuclear Cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1994-06-30</date><risdate>1994</risdate><volume>201</volume><issue>3</issue><spage>1109</spage><epage>1116</epage><pages>1109-1116</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>In previous papers we reported that D(−)-ribose and 2-deoxy-D-ribose, which rank at the top among reducing sugars, kill a variety of human and animal cells and cell lines [1, 2]. Here we demonstrate that these two sugars induce apoptosis in human quiescent peripheral blood mononuclear cells which are relatively insensitive to apoptosis. Apoptosis was assessed by morphological changes, DNA fragmentation by agarose gel electrophoresis and the appearance of an hypodiploid peak by flow cytometry. 2-deoxy-D-ribose was more potent than D(−)-ribose and apoptosis was evident from 48 hours of culture onwards. 2-deoxy-D-ribose-induced apoptosis was inibited by N-acetyl-L-cysteine, suggesting that glutathione metabolism and/or oxidative stress are involved in this type of apoptosis. Thus, D(−)-ribose and 2-deoxy-D-ribose can be useful tools to study the cellular and molecular events of apoptosis in human quiescent lymphocytes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8024552</pmid><doi>10.1006/bbrc.1994.1820</doi><tpages>8</tpages></addata></record> |
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| subjects | Acetylcysteine - pharmacology Apoptosis - drug effects Deoxyribose - pharmacology DNA Damage Humans Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - drug effects Ribose - pharmacology |
| title | D-Ribose and Deoxy-D-Ribose Induce Apoptosis in Human Quiescent Peripheral Blood Mononuclear Cells |
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