High density cultivation of hybridoma in charged porous carriers

A porous carrier whose surface is charged with polyethyleneimine (PEI) was evaluated for high-density cultivation of immobilized hybridoma cells. The carrier was used either in suspension or in fixed bed. For suspended carriers, the effect of carrier pore size, ion-exchange capacity (IEC), mixing in...

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Veröffentlicht in:	Journal of biotechnology 1994-05, Vol.34 (3), p.259-268
Hauptverfasser:	Ong, Celerina P., Pörtner, Ralf, Märkl, Herbert, Yamazaki, Yoshinori, Yasuda, Kimiaki, Matsumura, Masatoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Animals Antibodies, Monoclonal - biosynthesis Biological and medical sciences Biotechnology Biotechnology - instrumentation Cell Adhesion Cell Count Cell Division Cell hybridization, cell fusion, hybridomas Charged porous carrier Cytological Techniques - instrumentation Eukaryotic cell cultures Evaluation Studies as Topic Fixed bed reactor Fundamental and applied biological sciences. Psychology Glucose - metabolism Hybridoma Hybridomas - cytology Hybridomas - immunology Hybridomas - metabolism Ion Exchange Resins Methods. Procedures. Technologies Mice Particle Size Polyethyleneimine
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