Generation of Anaphylatoxin Activity Related to C3a, by Treatment of Human Serum with the Nitrogen Nucleophile N2H4 or the Chaotrope KSCN

The present study is concerned with the proteolytic processing of complement component C3 in normal human serum treated with N2M4 or KSCN in the presence of EDTA. Upon incubation with these agents. C3 is first converted to the thiolester‐cleaved form (C3i) and thereafter fragmented by factor I. In c...

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Veröffentlicht in:	Scandinavian journal of immunology 1985-12, Vol.22 (6), p.639-651
Hauptverfasser:	ZABERN, I., DAMERAU, B., NOLTE, R., GRÜNEFELD, E., VOGT, W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Anaphylatoxins - biosynthesis Applied sciences Complement C3 - immunology Complement C3 - metabolism Complement C3a Edetic Acid - pharmacology Exact sciences and technology Humans Hydrazines - pharmacology Ileum Immunoelectrophoresis Molecular Weight Muscle Contraction - drug effects Other techniques and industries Peptide Biosynthesis Peptide Hydrolases - metabolism Platelet Aggregation - drug effects Thiocyanates - pharmacology
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