Intraspecific molecular variation among Trichoderma harzianum isolates colonizing mushroom compost in the British Isles
Department of Applied lant Science, The Queen's University of Belfast, 1 and lant Pathology Research Division Department of Agriculture for Northern ieland, 2 Newforge Lane, belfast, BT9 5PX, UK ABSTRACT The genetic diversity in Trichoderma harzianum isolates from mushroom compost was assessed...
Gespeichert in:
| Veröffentlicht in: | Microbiology (Society for General Microbiology) 1994-04, Vol.140 (4), p.769-777 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 777 |
|---|---|
| container_issue | 4 |
| container_start_page | 769 |
| container_title | Microbiology (Society for General Microbiology) |
| container_volume | 140 |
| creator | Muthumeenakshi, S Mills, P. R Brownd, Averil E Seaby, D. A |
| description | Department of Applied lant Science, The Queen's University of Belfast, 1 and lant Pathology Research Division
Department of Agriculture for Northern ieland, 2 Newforge Lane, belfast, BT9 5PX, UK
ABSTRACT
The genetic diversity in Trichoderma harzianum isolates from mushroom compost was assessed using various molecular techniques. Restriction fragment length polymorphism (RFLP) analysis of ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) divided the 81 isolates into three major groups, 1, 2 and 3. There was no variation within a group in rDNA, while a low degree of polymorphism was detected in mtDNA. Random amplified polymorphic DNA (RAPD) analysis of 30 randomly chosen isolates, with six primers, in general confirmed the RFLP groups. Nucleotide sequence determination of rDNA internal transcribed spacer (ITS) 1 revealed three distinct ITS types, 1, 2 and 3, possessed by isolates from the respective groups 1, 2 and 3. Based on these molecular data, group 2 isolates, which are aggressive colonizers of mushroom compost, could be clearly distinguished from the isolates belonging to the other two groups.
1 Author for correspondence: P. R. Mills. Tel: +44 903 716123 ext. 2368. Fax: +44 903 726780.
Keywords: Trichoderma harzianum , mushroom compost, molecular variation, systematics
Present address: Horticulture Research International, Wellesbourne, Warwick CV35 9EF, UK. |
| doi_str_mv | 10.1099/00221287-140-4-769 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76570473</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16948245</sourcerecordid><originalsourceid>FETCH-LOGICAL-c435t-1a234835b9d72c45fcbabbec314ce530561224c40241823592380036ac08d0e43</originalsourceid><addsrcrecordid>eNqFkU1vFDEMhkcIVErhDyAh5YBQL9M6iTMfR1rxsVKlXso5ymQzO0bJZElmWtFfT9Au5diTLb-P_Vp6q-o9hwsOfX8JIAQXXVtzhBrrtulfVKccG1UL6OBl6aWCGrpWvK7e5PwToIjAT6qTtueCI55WD5t5SSbvnaWRLAvRO7t6k9i9SWQWijMzIc47dpfITnHrUjBsMumRzLwGRjl6s7jMbPRxpkcqZFjzlGIMZRb2MS-MZrZMjl0lWihPbJO9y2-rV6Px2b071rPqx9cvd9ff65vbb5vrzze1RamWmhshsZNq6LetsKhGO5hhcFZytE5JUA0XAi2CQN4JqXohOwDZGAvdFhzKs-rT4e4-xV-ry4sOlK3z3swurlm3jWoBW_ksyJseO4GqgOIA2hRzTm7U-0TBpN-ag_4bi_4Xiy6xaCwWfVn6cLy-DsFtn1aOORT941E32Ro_JjNbyk8YgkTkvGDnB2yi3fRAyemdmwOVTwaK5WH73_EPqlCkCw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16948245</pqid></control><display><type>article</type><title>Intraspecific molecular variation among Trichoderma harzianum isolates colonizing mushroom compost in the British Isles</title><source>MEDLINE</source><source>PubMed Central</source><creator>Muthumeenakshi, S ; Mills, P. R ; Brownd, Averil E ; Seaby, D. A</creator><creatorcontrib>Muthumeenakshi, S ; Mills, P. R ; Brownd, Averil E ; Seaby, D. A</creatorcontrib><description>Department of Applied lant Science, The Queen's University of Belfast, 1 and lant Pathology Research Division
Department of Agriculture for Northern ieland, 2 Newforge Lane, belfast, BT9 5PX, UK
ABSTRACT
The genetic diversity in Trichoderma harzianum isolates from mushroom compost was assessed using various molecular techniques. Restriction fragment length polymorphism (RFLP) analysis of ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) divided the 81 isolates into three major groups, 1, 2 and 3. There was no variation within a group in rDNA, while a low degree of polymorphism was detected in mtDNA. Random amplified polymorphic DNA (RAPD) analysis of 30 randomly chosen isolates, with six primers, in general confirmed the RFLP groups. Nucleotide sequence determination of rDNA internal transcribed spacer (ITS) 1 revealed three distinct ITS types, 1, 2 and 3, possessed by isolates from the respective groups 1, 2 and 3. Based on these molecular data, group 2 isolates, which are aggressive colonizers of mushroom compost, could be clearly distinguished from the isolates belonging to the other two groups.
1 Author for correspondence: P. R. Mills. Tel: +44 903 716123 ext. 2368. Fax: +44 903 726780.
Keywords: Trichoderma harzianum , mushroom compost, molecular variation, systematics
Present address: Horticulture Research International, Wellesbourne, Warwick CV35 9EF, UK.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/00221287-140-4-769</identifier><identifier>PMID: 7912144</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Base Sequence ; Basidiomycota ; Biological and medical sciences ; DNA, Fungal - genetics ; DNA, Mitochondrial - genetics ; DNA, Ribosomal - genetics ; England ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; Genetics of eukaryotes. Biological and molecular evolution ; Ireland ; Molecular Sequence Data ; Northern Ireland ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Population genetics, reproduction patterns ; Sequence Alignment ; Sequence Homology, Nucleic Acid ; Soil Microbiology ; Thallophyta, bryophyta ; Trichoderma - genetics ; Trichoderma - isolation & purification ; Trichoderma harzianum ; Vegetals</subject><ispartof>Microbiology (Society for General Microbiology), 1994-04, Vol.140 (4), p.769-777</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-1a234835b9d72c45fcbabbec314ce530561224c40241823592380036ac08d0e43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4034411$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7912144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Muthumeenakshi, S</creatorcontrib><creatorcontrib>Mills, P. R</creatorcontrib><creatorcontrib>Brownd, Averil E</creatorcontrib><creatorcontrib>Seaby, D. A</creatorcontrib><title>Intraspecific molecular variation among Trichoderma harzianum isolates colonizing mushroom compost in the British Isles</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>Department of Applied lant Science, The Queen's University of Belfast, 1 and lant Pathology Research Division
Department of Agriculture for Northern ieland, 2 Newforge Lane, belfast, BT9 5PX, UK
ABSTRACT
The genetic diversity in Trichoderma harzianum isolates from mushroom compost was assessed using various molecular techniques. Restriction fragment length polymorphism (RFLP) analysis of ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) divided the 81 isolates into three major groups, 1, 2 and 3. There was no variation within a group in rDNA, while a low degree of polymorphism was detected in mtDNA. Random amplified polymorphic DNA (RAPD) analysis of 30 randomly chosen isolates, with six primers, in general confirmed the RFLP groups. Nucleotide sequence determination of rDNA internal transcribed spacer (ITS) 1 revealed three distinct ITS types, 1, 2 and 3, possessed by isolates from the respective groups 1, 2 and 3. Based on these molecular data, group 2 isolates, which are aggressive colonizers of mushroom compost, could be clearly distinguished from the isolates belonging to the other two groups.
1 Author for correspondence: P. R. Mills. Tel: +44 903 716123 ext. 2368. Fax: +44 903 726780.
Keywords: Trichoderma harzianum , mushroom compost, molecular variation, systematics
Present address: Horticulture Research International, Wellesbourne, Warwick CV35 9EF, UK.</description><subject>Base Sequence</subject><subject>Basidiomycota</subject><subject>Biological and medical sciences</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Mitochondrial - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>England</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Ireland</subject><subject>Molecular Sequence Data</subject><subject>Northern Ireland</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Population genetics, reproduction patterns</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Soil Microbiology</subject><subject>Thallophyta, bryophyta</subject><subject>Trichoderma - genetics</subject><subject>Trichoderma - isolation & purification</subject><subject>Trichoderma harzianum</subject><subject>Vegetals</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vFDEMhkcIVErhDyAh5YBQL9M6iTMfR1rxsVKlXso5ymQzO0bJZElmWtFfT9Au5diTLb-P_Vp6q-o9hwsOfX8JIAQXXVtzhBrrtulfVKccG1UL6OBl6aWCGrpWvK7e5PwToIjAT6qTtueCI55WD5t5SSbvnaWRLAvRO7t6k9i9SWQWijMzIc47dpfITnHrUjBsMumRzLwGRjl6s7jMbPRxpkcqZFjzlGIMZRb2MS-MZrZMjl0lWihPbJO9y2-rV6Px2b071rPqx9cvd9ff65vbb5vrzze1RamWmhshsZNq6LetsKhGO5hhcFZytE5JUA0XAi2CQN4JqXohOwDZGAvdFhzKs-rT4e4-xV-ry4sOlK3z3swurlm3jWoBW_ksyJseO4GqgOIA2hRzTm7U-0TBpN-ag_4bi_4Xiy6xaCwWfVn6cLy-DsFtn1aOORT941E32Ro_JjNbyk8YgkTkvGDnB2yi3fRAyemdmwOVTwaK5WH73_EPqlCkCw</recordid><startdate>19940401</startdate><enddate>19940401</enddate><creator>Muthumeenakshi, S</creator><creator>Mills, P. R</creator><creator>Brownd, Averil E</creator><creator>Seaby, D. A</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19940401</creationdate><title>Intraspecific molecular variation among Trichoderma harzianum isolates colonizing mushroom compost in the British Isles</title><author>Muthumeenakshi, S ; Mills, P. R ; Brownd, Averil E ; Seaby, D. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-1a234835b9d72c45fcbabbec314ce530561224c40241823592380036ac08d0e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Base Sequence</topic><topic>Basidiomycota</topic><topic>Biological and medical sciences</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Mitochondrial - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>England</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Ireland</topic><topic>Molecular Sequence Data</topic><topic>Northern Ireland</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Population genetics, reproduction patterns</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Soil Microbiology</topic><topic>Thallophyta, bryophyta</topic><topic>Trichoderma - genetics</topic><topic>Trichoderma - isolation & purification</topic><topic>Trichoderma harzianum</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muthumeenakshi, S</creatorcontrib><creatorcontrib>Mills, P. R</creatorcontrib><creatorcontrib>Brownd, Averil E</creatorcontrib><creatorcontrib>Seaby, D. A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muthumeenakshi, S</au><au>Mills, P. R</au><au>Brownd, Averil E</au><au>Seaby, D. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intraspecific molecular variation among Trichoderma harzianum isolates colonizing mushroom compost in the British Isles</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>1994-04-01</date><risdate>1994</risdate><volume>140</volume><issue>4</issue><spage>769</spage><epage>777</epage><pages>769-777</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>Department of Applied lant Science, The Queen's University of Belfast, 1 and lant Pathology Research Division
Department of Agriculture for Northern ieland, 2 Newforge Lane, belfast, BT9 5PX, UK
ABSTRACT
The genetic diversity in Trichoderma harzianum isolates from mushroom compost was assessed using various molecular techniques. Restriction fragment length polymorphism (RFLP) analysis of ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) divided the 81 isolates into three major groups, 1, 2 and 3. There was no variation within a group in rDNA, while a low degree of polymorphism was detected in mtDNA. Random amplified polymorphic DNA (RAPD) analysis of 30 randomly chosen isolates, with six primers, in general confirmed the RFLP groups. Nucleotide sequence determination of rDNA internal transcribed spacer (ITS) 1 revealed three distinct ITS types, 1, 2 and 3, possessed by isolates from the respective groups 1, 2 and 3. Based on these molecular data, group 2 isolates, which are aggressive colonizers of mushroom compost, could be clearly distinguished from the isolates belonging to the other two groups.
1 Author for correspondence: P. R. Mills. Tel: +44 903 716123 ext. 2368. Fax: +44 903 726780.
Keywords: Trichoderma harzianum , mushroom compost, molecular variation, systematics
Present address: Horticulture Research International, Wellesbourne, Warwick CV35 9EF, UK.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>7912144</pmid><doi>10.1099/00221287-140-4-769</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1350-0872 |
| ispartof | Microbiology (Society for General Microbiology), 1994-04, Vol.140 (4), p.769-777 |
| issn | 1350-0872 1465-2080 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76570473 |
| source | MEDLINE; PubMed Central |
| subjects | Base Sequence Basidiomycota Biological and medical sciences DNA, Fungal - genetics DNA, Mitochondrial - genetics DNA, Ribosomal - genetics England Fundamental and applied biological sciences. Psychology Genetic Variation Genetics of eukaryotes. Biological and molecular evolution Ireland Molecular Sequence Data Northern Ireland Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Population genetics, reproduction patterns Sequence Alignment Sequence Homology, Nucleic Acid Soil Microbiology Thallophyta, bryophyta Trichoderma - genetics Trichoderma - isolation & purification Trichoderma harzianum Vegetals |
| title | Intraspecific molecular variation among Trichoderma harzianum isolates colonizing mushroom compost in the British Isles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T07%3A19%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Intraspecific%20molecular%20variation%20among%20Trichoderma%20harzianum%20isolates%20colonizing%20mushroom%20compost%20in%20the%20British%20Isles&rft.jtitle=Microbiology%20(Society%20for%20General%20Microbiology)&rft.au=Muthumeenakshi,%20S&rft.date=1994-04-01&rft.volume=140&rft.issue=4&rft.spage=769&rft.epage=777&rft.pages=769-777&rft.issn=1350-0872&rft.eissn=1465-2080&rft_id=info:doi/10.1099/00221287-140-4-769&rft_dat=%3Cproquest_cross%3E16948245%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16948245&rft_id=info:pmid/7912144&rfr_iscdi=true |