Proline-Rich Domain and Glycosylation Are Not Essential for the Enzymic Activity of Bile Salt-Activated Lipase. Kinetic Studies of T-BAL, a Truncated Form of the Enzyme, Expressed in Escherichia coli

We have expressed and purified a truncated recombinant human milk bile salt-activated lipase (T-BAL) from the T7 expression system in Escherichia coli. This T-BAL contains the N-terminal 538 residues of the 722-residue native enzyme. The purified T-BAL, when assayed with PANA (p-nitrophenyl acetate)...

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