Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus
A restriction fragment containing the granulin gene from the Trichoplusia ni granulosis virus was located in a blot of viral genomic DNA using a cloned polyhedrin gene as a probe. This fragment was cloned, mapped, subcloned, and the sequence of the coding region and 5′ and 3′ flanking regions was de...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1985-03, Vol.141 (2), p.328-332 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Akiyoshi, D. Chakerian, R. Rohrmann, G.F. Nesson, M.H. Beaudreau, G.S. |
| description | A restriction fragment containing the granulin gene from the
Trichoplusia ni granulosis virus was located in a blot of viral genomic DNA using a cloned polyhedrin gene as a probe. This fragment was cloned, mapped, subcloned, and the sequence of the coding region and 5′ and 3′ flanking regions was determined. Although granulin is very similar in size to nuclear polyhedrosis virus polyhedrins, the N-terminal region of granulin demonstrated a high degree of variability with the first 60 amino acids only 28% homologous to the
Autographa californica nuclear polyhedrosis virus (AcMNPV) polyhedrin sequence. Between amino acid 60 and the carboxyterminus at amino acid 248, the sequence was very similar (64%) to polyhedrin sequences. Overall the nucleotide and amino acid sequences were 58 and 53%, respectively, related to those of AcMNPV. No introns were evident in the gene. |
| doi_str_mv | 10.1016/0042-6822(85)90267-3 |
| format | Article |
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Trichoplusia ni granulosis virus was located in a blot of viral genomic DNA using a cloned polyhedrin gene as a probe. This fragment was cloned, mapped, subcloned, and the sequence of the coding region and 5′ and 3′ flanking regions was determined. Although granulin is very similar in size to nuclear polyhedrosis virus polyhedrins, the N-terminal region of granulin demonstrated a high degree of variability with the first 60 amino acids only 28% homologous to the
Autographa californica nuclear polyhedrosis virus (AcMNPV) polyhedrin sequence. Between amino acid 60 and the carboxyterminus at amino acid 248, the sequence was very similar (64%) to polyhedrin sequences. Overall the nucleotide and amino acid sequences were 58 and 53%, respectively, related to those of AcMNPV. No introns were evident in the gene.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(85)90267-3</identifier><identifier>PMID: 4082501</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; DNA, Viral ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Viral ; Genetics ; granulosis virus ; Insect Viruses - genetics ; Microbiology ; Viral Matrix Proteins ; Viral Proteins - genetics ; Virology</subject><ispartof>Virology (New York, N.Y.), 1985-03, Vol.141 (2), p.328-332</ispartof><rights>1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-a95389007359bf5c7ba129eeb8fb42e756f4793d9d3de90e627fbd8886ef2e3e3</citedby><cites>FETCH-LOGICAL-c483t-a95389007359bf5c7ba129eeb8fb42e756f4793d9d3de90e627fbd8886ef2e3e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0042682285902673$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8420203$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4082501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akiyoshi, D.</creatorcontrib><creatorcontrib>Chakerian, R.</creatorcontrib><creatorcontrib>Rohrmann, G.F.</creatorcontrib><creatorcontrib>Nesson, M.H.</creatorcontrib><creatorcontrib>Beaudreau, G.S.</creatorcontrib><title>Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>A restriction fragment containing the granulin gene from the
Trichoplusia ni granulosis virus was located in a blot of viral genomic DNA using a cloned polyhedrin gene as a probe. This fragment was cloned, mapped, subcloned, and the sequence of the coding region and 5′ and 3′ flanking regions was determined. Although granulin is very similar in size to nuclear polyhedrosis virus polyhedrins, the N-terminal region of granulin demonstrated a high degree of variability with the first 60 amino acids only 28% homologous to the
Autographa californica nuclear polyhedrosis virus (AcMNPV) polyhedrin sequence. Between amino acid 60 and the carboxyterminus at amino acid 248, the sequence was very similar (64%) to polyhedrin sequences. Overall the nucleotide and amino acid sequences were 58 and 53%, respectively, related to those of AcMNPV. No introns were evident in the gene.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA, Viral</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Viral</subject><subject>Genetics</subject><subject>granulosis virus</subject><subject>Insect Viruses - genetics</subject><subject>Microbiology</subject><subject>Viral Matrix Proteins</subject><subject>Viral Proteins - genetics</subject><subject>Virology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo6-zqP1Dog4geWvP9cVmQwVVhwcvuOaTTldlITzIm0wv-e9M7zRz1FCr11EvxFEJvCP5EMJGfMea0l5rSD1p8NJhK1bNnaEOwkT1mnDxHmzPyEl3W-gu3Wil8gS441lRgskH32ymnmHadS2NX4fcMyS9lDt3xAbpdcWmeYup2kKALJe-fvu9K9A_5MM01ui7FFcs11u4xlrm-Qi-Cmyq8Xt8rdH_z9W77vb_9-e3H9stt77lmx94ZwbTBWDFhhiC8GhyhBmDQYeAUlJCBK8NGM7IRDAZJVRhGrbWEQIEBu0LvT7mHktvq9Wj3sXqYJpcgz9UqKSTlgvwXJJw1f4o2kJ9AX3KtBYI9lLh35Y8l2C7a7eLULk6tFvZJu2Vt7O2aPw97GM9Dq-fWf7f2XfVuCs2Xj_WMaU4xxUvM9QmDJu0xQrHVx3YRGGMBf7Rjjv_e4y_DMp4n</recordid><startdate>198503</startdate><enddate>198503</enddate><creator>Akiyoshi, D.</creator><creator>Chakerian, R.</creator><creator>Rohrmann, G.F.</creator><creator>Nesson, M.H.</creator><creator>Beaudreau, G.S.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198503</creationdate><title>Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus</title><author>Akiyoshi, D. ; Chakerian, R. ; Rohrmann, G.F. ; Nesson, M.H. ; Beaudreau, G.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-a95389007359bf5c7ba129eeb8fb42e756f4793d9d3de90e627fbd8886ef2e3e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>DNA, Viral</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Viral</topic><topic>Genetics</topic><topic>granulosis virus</topic><topic>Insect Viruses - genetics</topic><topic>Microbiology</topic><topic>Viral Matrix Proteins</topic><topic>Viral Proteins - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akiyoshi, D.</creatorcontrib><creatorcontrib>Chakerian, R.</creatorcontrib><creatorcontrib>Rohrmann, G.F.</creatorcontrib><creatorcontrib>Nesson, M.H.</creatorcontrib><creatorcontrib>Beaudreau, G.S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akiyoshi, D.</au><au>Chakerian, R.</au><au>Rohrmann, G.F.</au><au>Nesson, M.H.</au><au>Beaudreau, G.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1985-03</date><risdate>1985</risdate><volume>141</volume><issue>2</issue><spage>328</spage><epage>332</epage><pages>328-332</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>A restriction fragment containing the granulin gene from the
Trichoplusia ni granulosis virus was located in a blot of viral genomic DNA using a cloned polyhedrin gene as a probe. This fragment was cloned, mapped, subcloned, and the sequence of the coding region and 5′ and 3′ flanking regions was determined. Although granulin is very similar in size to nuclear polyhedrosis virus polyhedrins, the N-terminal region of granulin demonstrated a high degree of variability with the first 60 amino acids only 28% homologous to the
Autographa californica nuclear polyhedrosis virus (AcMNPV) polyhedrin sequence. Between amino acid 60 and the carboxyterminus at amino acid 248, the sequence was very similar (64%) to polyhedrin sequences. Overall the nucleotide and amino acid sequences were 58 and 53%, respectively, related to those of AcMNPV. No introns were evident in the gene.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>4082501</pmid><doi>10.1016/0042-6822(85)90267-3</doi><tpages>5</tpages></addata></record> |
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| ispartof | Virology (New York, N.Y.), 1985-03, Vol.141 (2), p.328-332 |
| issn | 0042-6822 1096-0341 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB Electronic Journals Library |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Cloning, Molecular DNA, Viral Fundamental and applied biological sciences. Psychology Genes Genes, Viral Genetics granulosis virus Insect Viruses - genetics Microbiology Viral Matrix Proteins Viral Proteins - genetics Virology |
| title | Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus |
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