Growth of wool follicles in culture
A procedure for the culture of isolated wool follicles from Merino sheep is described. Follicles were microdissected from midside skin samples of 2-yr-old wethers and transferred, individually, to 24-well tissue culture plates. When maintained in supplemented Williams' E medium containing 5 to...
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Veröffentlicht in: | In vitro cellular & developmental biology. Animal 1994-02, Vol.30A (2), p.90-98 |
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description | A procedure for the culture of isolated wool follicles from Merino sheep is described. Follicles were microdissected from midside skin samples of 2-yr-old wethers and transferred, individually, to 24-well tissue culture plates. When maintained in supplemented Williams' E medium containing 5 to 10% fetal bovine serum (FBS), insulin, hydrocortisone, and a trace element mixture, fiber growth rates of 40 to$80 \mu m/day$were observed. Follicles maintained their morphologic integrity for up to 7 days, incorporated$[methyl-^{3}H]thymidine$into DNA and [35S]methionine into intermediate-filament keratins of the growing fiber. Insulin and hydrocortisone stimulated fiber growth at concentrations of$10 \mu g/ml$and 50 ng/ml, respectively, but higher doses were inhibitory. The growth of fibers in response to hydrocortisone and the changes in follicle morphology was similar to those induced in skin after systemic administration of cortisol in vivo. A positive interaction between hydrocortisone and trace elements for follicle survival and hydrocortisone, insulin, and FBS for fiber growth was also found. The successful culture of Merino sheep follicles provides a model with which to study the direct influence of endocrine, nutritional and local factors on wool keratin synthesis independently of systemic shifts in the animals' metabolism. |
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Follicles were microdissected from midside skin samples of 2-yr-old wethers and transferred, individually, to 24-well tissue culture plates. When maintained in supplemented Williams' E medium containing 5 to 10% fetal bovine serum (FBS), insulin, hydrocortisone, and a trace element mixture, fiber growth rates of 40 to$80 \mu m/day$were observed. Follicles maintained their morphologic integrity for up to 7 days, incorporated$[methyl-^{3}H]thymidine$into DNA and [35S]methionine into intermediate-filament keratins of the growing fiber. Insulin and hydrocortisone stimulated fiber growth at concentrations of$10 \mu g/ml$and 50 ng/ml, respectively, but higher doses were inhibitory. The growth of fibers in response to hydrocortisone and the changes in follicle morphology was similar to those induced in skin after systemic administration of cortisol in vivo. A positive interaction between hydrocortisone and trace elements for follicle survival and hydrocortisone, insulin, and FBS for fiber growth was also found. The successful culture of Merino sheep follicles provides a model with which to study the direct influence of endocrine, nutritional and local factors on wool keratin synthesis independently of systemic shifts in the animals' metabolism.</description><identifier>ISSN: 1071-2690</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/BF02631399</identifier><identifier>PMID: 7516798</identifier><language>eng</language><publisher>Largo, MD: Tissue Culture Association, Inc</publisher><subject>Aging ; Animal cells ; Animals ; Biological and medical sciences ; Biotechnology ; cell culture ; Cell Division ; Cellular Models ; Culture Media ; Culture Techniques ; DNA - biosynthesis ; Establishment of new cell lines, improvement of cultural methods, mass cultures ; Eukaryotic cell cultures ; Female ; Fetal Blood ; Follicles ; Fundamental and applied biological sciences. Psychology ; Hair ; Hair - drug effects ; Hair - growth & development ; Hair - metabolism ; Hair follicles ; Human growth ; Hydrocortisone - pharmacology ; Insulin ; Insulin - pharmacology ; Keratins ; Keratins - biosynthesis ; Methods. Procedures. Technologies ; Models, Biological ; Papillae ; Sheep ; Skin ; Wool</subject><ispartof>In vitro cellular & developmental biology. Animal, 1994-02, Vol.30A (2), p.90-98</ispartof><rights>Copyright 1993 Tissue Culture Association</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-d560d4a81a25984a8daecd1bb0fc2954e9d484621da4ea7b8a0beaf913738b73</citedby><cites>FETCH-LOGICAL-c356t-d560d4a81a25984a8daecd1bb0fc2954e9d484621da4ea7b8a0beaf913738b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4294193$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4294193$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4271709$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7516798$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bond, J.J</creatorcontrib><creatorcontrib>Wynn, P.C</creatorcontrib><creatorcontrib>Brown, G.N</creatorcontrib><creatorcontrib>Moore, G.P.M</creatorcontrib><title>Growth of wool follicles in culture</title><title>In vitro cellular & developmental biology. Animal</title><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>A procedure for the culture of isolated wool follicles from Merino sheep is described. Follicles were microdissected from midside skin samples of 2-yr-old wethers and transferred, individually, to 24-well tissue culture plates. When maintained in supplemented Williams' E medium containing 5 to 10% fetal bovine serum (FBS), insulin, hydrocortisone, and a trace element mixture, fiber growth rates of 40 to$80 \mu m/day$were observed. Follicles maintained their morphologic integrity for up to 7 days, incorporated$[methyl-^{3}H]thymidine$into DNA and [35S]methionine into intermediate-filament keratins of the growing fiber. Insulin and hydrocortisone stimulated fiber growth at concentrations of$10 \mu g/ml$and 50 ng/ml, respectively, but higher doses were inhibitory. The growth of fibers in response to hydrocortisone and the changes in follicle morphology was similar to those induced in skin after systemic administration of cortisol in vivo. A positive interaction between hydrocortisone and trace elements for follicle survival and hydrocortisone, insulin, and FBS for fiber growth was also found. The successful culture of Merino sheep follicles provides a model with which to study the direct influence of endocrine, nutritional and local factors on wool keratin synthesis independently of systemic shifts in the animals' metabolism.</description><subject>Aging</subject><subject>Animal cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cell culture</subject><subject>Cell Division</subject><subject>Cellular Models</subject><subject>Culture Media</subject><subject>Culture Techniques</subject><subject>DNA - biosynthesis</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Female</subject><subject>Fetal Blood</subject><subject>Follicles</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hair</subject><subject>Hair - drug effects</subject><subject>Hair - growth & development</subject><subject>Hair - metabolism</subject><subject>Hair follicles</subject><subject>Human growth</subject><subject>Hydrocortisone - pharmacology</subject><subject>Insulin</subject><subject>Insulin - pharmacology</subject><subject>Keratins</subject><subject>Keratins - biosynthesis</subject><subject>Methods. Procedures. Technologies</subject><subject>Models, Biological</subject><subject>Papillae</subject><subject>Sheep</subject><subject>Skin</subject><subject>Wool</subject><issn>1071-2690</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkMFLwzAUh4MoU6cXz4oFxYNQzUvSpDnqcFMYeHCCt5KmqXZky0xahv-9GR3Td3kPvo_fgx9CZ4DvAGNx_zjGhFOgUu6hI8gYTQXmH_vxxgJSwiU-RMchzHEcCXyABiIDLmR-hK4m3q3br8TVydo5m9TO2kZbE5JmmejOtp03J-igVjaY0-0eotn4aTZ6Tqevk5fRwzTVNONtWmUcV0zloEgm83hUyugKyhLXmsiMGVmxnHEClWJGiTJXuDSqlkAFzUtBh-imj115992Z0BaLJmhjrVoa14VC8IwDxySKt72ovQvBm7pY-Wah_E8BuNgUUvwVEuWLbWpXLky1U7cNRH695SpoZWuvlroJO40RAQJvYs57bR5a5_9hyUDSiC97XCtXqE8fE97fCAaKgeXxFaG_H1N4bQ</recordid><startdate>19940201</startdate><enddate>19940201</enddate><creator>Bond, J.J</creator><creator>Wynn, P.C</creator><creator>Brown, G.N</creator><creator>Moore, G.P.M</creator><general>Tissue Culture Association, Inc</general><general>Society for In Vitro Biology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940201</creationdate><title>Growth of wool follicles in culture</title><author>Bond, J.J ; Wynn, P.C ; Brown, G.N ; Moore, G.P.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-d560d4a81a25984a8daecd1bb0fc2954e9d484621da4ea7b8a0beaf913738b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Aging</topic><topic>Animal cells</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cell culture</topic><topic>Cell Division</topic><topic>Cellular Models</topic><topic>Culture Media</topic><topic>Culture Techniques</topic><topic>DNA - biosynthesis</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass cultures</topic><topic>Eukaryotic cell cultures</topic><topic>Female</topic><topic>Fetal Blood</topic><topic>Follicles</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hair</topic><topic>Hair - drug effects</topic><topic>Hair - growth & development</topic><topic>Hair - metabolism</topic><topic>Hair follicles</topic><topic>Human growth</topic><topic>Hydrocortisone - pharmacology</topic><topic>Insulin</topic><topic>Insulin - pharmacology</topic><topic>Keratins</topic><topic>Keratins - biosynthesis</topic><topic>Methods. Procedures. Technologies</topic><topic>Models, Biological</topic><topic>Papillae</topic><topic>Sheep</topic><topic>Skin</topic><topic>Wool</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bond, J.J</creatorcontrib><creatorcontrib>Wynn, P.C</creatorcontrib><creatorcontrib>Brown, G.N</creatorcontrib><creatorcontrib>Moore, G.P.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>In vitro cellular & developmental biology. Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bond, J.J</au><au>Wynn, P.C</au><au>Brown, G.N</au><au>Moore, G.P.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth of wool follicles in culture</atitle><jtitle>In vitro cellular & developmental biology. Animal</jtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>1994-02-01</date><risdate>1994</risdate><volume>30A</volume><issue>2</issue><spage>90</spage><epage>98</epage><pages>90-98</pages><issn>1071-2690</issn><eissn>1543-706X</eissn><abstract>A procedure for the culture of isolated wool follicles from Merino sheep is described. Follicles were microdissected from midside skin samples of 2-yr-old wethers and transferred, individually, to 24-well tissue culture plates. When maintained in supplemented Williams' E medium containing 5 to 10% fetal bovine serum (FBS), insulin, hydrocortisone, and a trace element mixture, fiber growth rates of 40 to$80 \mu m/day$were observed. Follicles maintained their morphologic integrity for up to 7 days, incorporated$[methyl-^{3}H]thymidine$into DNA and [35S]methionine into intermediate-filament keratins of the growing fiber. Insulin and hydrocortisone stimulated fiber growth at concentrations of$10 \mu g/ml$and 50 ng/ml, respectively, but higher doses were inhibitory. The growth of fibers in response to hydrocortisone and the changes in follicle morphology was similar to those induced in skin after systemic administration of cortisol in vivo. A positive interaction between hydrocortisone and trace elements for follicle survival and hydrocortisone, insulin, and FBS for fiber growth was also found. The successful culture of Merino sheep follicles provides a model with which to study the direct influence of endocrine, nutritional and local factors on wool keratin synthesis independently of systemic shifts in the animals' metabolism.</abstract><cop>Largo, MD</cop><pub>Tissue Culture Association, Inc</pub><pmid>7516798</pmid><doi>10.1007/BF02631399</doi><tpages>9</tpages></addata></record> |
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subjects | Aging Animal cells Animals Biological and medical sciences Biotechnology cell culture Cell Division Cellular Models Culture Media Culture Techniques DNA - biosynthesis Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Female Fetal Blood Follicles Fundamental and applied biological sciences. Psychology Hair Hair - drug effects Hair - growth & development Hair - metabolism Hair follicles Human growth Hydrocortisone - pharmacology Insulin Insulin - pharmacology Keratins Keratins - biosynthesis Methods. Procedures. Technologies Models, Biological Papillae Sheep Skin Wool |
title | Growth of wool follicles in culture |
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