Growth of wool follicles in culture

A procedure for the culture of isolated wool follicles from Merino sheep is described. Follicles were microdissected from midside skin samples of 2-yr-old wethers and transferred, individually, to 24-well tissue culture plates. When maintained in supplemented Williams' E medium containing 5 to...

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Veröffentlicht in:In vitro cellular & developmental biology. Animal 1994-02, Vol.30A (2), p.90-98
Hauptverfasser: Bond, J.J, Wynn, P.C, Brown, G.N, Moore, G.P.M
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container_title In vitro cellular & developmental biology. Animal
container_volume 30A
creator Bond, J.J
Wynn, P.C
Brown, G.N
Moore, G.P.M
description A procedure for the culture of isolated wool follicles from Merino sheep is described. Follicles were microdissected from midside skin samples of 2-yr-old wethers and transferred, individually, to 24-well tissue culture plates. When maintained in supplemented Williams' E medium containing 5 to 10% fetal bovine serum (FBS), insulin, hydrocortisone, and a trace element mixture, fiber growth rates of 40 to$80 \mu m/day$were observed. Follicles maintained their morphologic integrity for up to 7 days, incorporated$[methyl-^{3}H]thymidine$into DNA and [35S]methionine into intermediate-filament keratins of the growing fiber. Insulin and hydrocortisone stimulated fiber growth at concentrations of$10 \mu g/ml$and 50 ng/ml, respectively, but higher doses were inhibitory. The growth of fibers in response to hydrocortisone and the changes in follicle morphology was similar to those induced in skin after systemic administration of cortisol in vivo. A positive interaction between hydrocortisone and trace elements for follicle survival and hydrocortisone, insulin, and FBS for fiber growth was also found. The successful culture of Merino sheep follicles provides a model with which to study the direct influence of endocrine, nutritional and local factors on wool keratin synthesis independently of systemic shifts in the animals' metabolism.
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Follicles were microdissected from midside skin samples of 2-yr-old wethers and transferred, individually, to 24-well tissue culture plates. When maintained in supplemented Williams' E medium containing 5 to 10% fetal bovine serum (FBS), insulin, hydrocortisone, and a trace element mixture, fiber growth rates of 40 to$80 \mu m/day$were observed. Follicles maintained their morphologic integrity for up to 7 days, incorporated$[methyl-^{3}H]thymidine$into DNA and [35S]methionine into intermediate-filament keratins of the growing fiber. Insulin and hydrocortisone stimulated fiber growth at concentrations of$10 \mu g/ml$and 50 ng/ml, respectively, but higher doses were inhibitory. The growth of fibers in response to hydrocortisone and the changes in follicle morphology was similar to those induced in skin after systemic administration of cortisol in vivo. A positive interaction between hydrocortisone and trace elements for follicle survival and hydrocortisone, insulin, and FBS for fiber growth was also found. 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Psychology ; Hair ; Hair - drug effects ; Hair - growth &amp; development ; Hair - metabolism ; Hair follicles ; Human growth ; Hydrocortisone - pharmacology ; Insulin ; Insulin - pharmacology ; Keratins ; Keratins - biosynthesis ; Methods. Procedures. Technologies ; Models, Biological ; Papillae ; Sheep ; Skin ; Wool</subject><ispartof>In vitro cellular &amp; developmental biology. 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Animal</title><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>A procedure for the culture of isolated wool follicles from Merino sheep is described. Follicles were microdissected from midside skin samples of 2-yr-old wethers and transferred, individually, to 24-well tissue culture plates. When maintained in supplemented Williams' E medium containing 5 to 10% fetal bovine serum (FBS), insulin, hydrocortisone, and a trace element mixture, fiber growth rates of 40 to$80 \mu m/day$were observed. Follicles maintained their morphologic integrity for up to 7 days, incorporated$[methyl-^{3}H]thymidine$into DNA and [35S]methionine into intermediate-filament keratins of the growing fiber. Insulin and hydrocortisone stimulated fiber growth at concentrations of$10 \mu g/ml$and 50 ng/ml, respectively, but higher doses were inhibitory. 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The successful culture of Merino sheep follicles provides a model with which to study the direct influence of endocrine, nutritional and local factors on wool keratin synthesis independently of systemic shifts in the animals' metabolism.</description><subject>Aging</subject><subject>Animal cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cell culture</subject><subject>Cell Division</subject><subject>Cellular Models</subject><subject>Culture Media</subject><subject>Culture Techniques</subject><subject>DNA - biosynthesis</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Female</subject><subject>Fetal Blood</subject><subject>Follicles</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Hair</topic><topic>Hair - drug effects</topic><topic>Hair - growth &amp; development</topic><topic>Hair - metabolism</topic><topic>Hair follicles</topic><topic>Human growth</topic><topic>Hydrocortisone - pharmacology</topic><topic>Insulin</topic><topic>Insulin - pharmacology</topic><topic>Keratins</topic><topic>Keratins - biosynthesis</topic><topic>Methods. Procedures. Technologies</topic><topic>Models, Biological</topic><topic>Papillae</topic><topic>Sheep</topic><topic>Skin</topic><topic>Wool</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bond, J.J</creatorcontrib><creatorcontrib>Wynn, P.C</creatorcontrib><creatorcontrib>Brown, G.N</creatorcontrib><creatorcontrib>Moore, G.P.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>In vitro cellular &amp; developmental biology. 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When maintained in supplemented Williams' E medium containing 5 to 10% fetal bovine serum (FBS), insulin, hydrocortisone, and a trace element mixture, fiber growth rates of 40 to$80 \mu m/day$were observed. Follicles maintained their morphologic integrity for up to 7 days, incorporated$[methyl-^{3}H]thymidine$into DNA and [35S]methionine into intermediate-filament keratins of the growing fiber. Insulin and hydrocortisone stimulated fiber growth at concentrations of$10 \mu g/ml$and 50 ng/ml, respectively, but higher doses were inhibitory. The growth of fibers in response to hydrocortisone and the changes in follicle morphology was similar to those induced in skin after systemic administration of cortisol in vivo. A positive interaction between hydrocortisone and trace elements for follicle survival and hydrocortisone, insulin, and FBS for fiber growth was also found. The successful culture of Merino sheep follicles provides a model with which to study the direct influence of endocrine, nutritional and local factors on wool keratin synthesis independently of systemic shifts in the animals' metabolism.</abstract><cop>Largo, MD</cop><pub>Tissue Culture Association, Inc</pub><pmid>7516798</pmid><doi>10.1007/BF02631399</doi><tpages>9</tpages></addata></record>
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source MEDLINE; JSTOR Archive Collection A-Z Listing; SpringerLink Journals - AutoHoldings
subjects Aging
Animal cells
Animals
Biological and medical sciences
Biotechnology
cell culture
Cell Division
Cellular Models
Culture Media
Culture Techniques
DNA - biosynthesis
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Female
Fetal Blood
Follicles
Fundamental and applied biological sciences. Psychology
Hair
Hair - drug effects
Hair - growth & development
Hair - metabolism
Hair follicles
Human growth
Hydrocortisone - pharmacology
Insulin
Insulin - pharmacology
Keratins
Keratins - biosynthesis
Methods. Procedures. Technologies
Models, Biological
Papillae
Sheep
Skin
Wool
title Growth of wool follicles in culture
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