Analysis of the Schizosaccharomyces pombe cyclin puc1: evidence for a role in cell cycle exit
The puc1+ gene, encoding a G1-type cyclin from the fission yeast Schizosaccharomyces pombe, was originally isolated by complementation in the budding yeast Saccharomyces cerevisiae. Here, we report the molecular characterization of this gene and analyse its role in S. pombe. We fail to identify any...
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| Veröffentlicht in: | Journal of cell science 1994-03, Vol.107 (3), p.601-613 |
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| creator | Forsburg, S.L Nurse, P |
| description | The puc1+ gene, encoding a G1-type cyclin from the fission yeast Schizosaccharomyces pombe, was originally isolated by complementation in the budding yeast Saccharomyces cerevisiae. Here, we report the molecular characterization of this gene and analyse its role in S. pombe. We fail to identify any function of this cyclin at the mitotic G1/S transition in S. pombe, but demonstrate that it does function in exit from the mitotic cycle. Expression of the puc1+ gene is increased during nitrogen starvation, and puc1 affects the timing of sexual development in response to starvation. Overexpression of the puc1 protein blocks sexual development, and rescues pat1ts cells, which would otherwise undergo a lethal meiosis. We conclude that puc1 contributes to negative regulation of the timing of sexual development in fission yeast, and functions at the transition between cycling and non-cycling cells. |
| doi_str_mv | 10.1242/jcs.107.3.601 |
| format | Article |
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Here, we report the molecular characterization of this gene and analyse its role in S. pombe. We fail to identify any function of this cyclin at the mitotic G1/S transition in S. pombe, but demonstrate that it does function in exit from the mitotic cycle. Expression of the puc1+ gene is increased during nitrogen starvation, and puc1 affects the timing of sexual development in response to starvation. Overexpression of the puc1 protein blocks sexual development, and rescues pat1ts cells, which would otherwise undergo a lethal meiosis. We conclude that puc1 contributes to negative regulation of the timing of sexual development in fission yeast, and functions at the transition between cycling and non-cycling cells.</description><identifier>ISSN: 0021-9533</identifier><identifier>EISSN: 1477-9137</identifier><identifier>DOI: 10.1242/jcs.107.3.601</identifier><identifier>PMID: 8006074</identifier><identifier>CODEN: JNCSAI</identifier><language>eng</language><publisher>Cambridge: Company of Biologists</publisher><subject>Alleles ; Amino Acid Sequence ; amino acid sequences ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Cell Cycle - genetics ; Cell cycle, cell proliferation ; Cell physiology ; Cloning, Molecular ; complementary DNA ; Cyclins - analysis ; Cyclins - genetics ; DNA, Fungal ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - analysis ; Fungal Proteins - genetics ; genbank/x74451 ; gene expression ; Genes, Fungal ; Immunoblotting ; meiosis ; messenger RNA ; mitosis ; Molecular and cellular biology ; Molecular Sequence Data ; nucleotide sequences ; Phenotype ; proteins ; puc1 gene ; Reproduction - genetics ; Saccharomycetales ; Schizosaccharomyces - chemistry ; Schizosaccharomyces - cytology ; Schizosaccharomyces - genetics ; Schizosaccharomyces - growth & development ; Schizosaccharomyces pombe ; Schizosaccharomyces pombe Proteins ; sexual development ; structural genes</subject><ispartof>Journal of cell science, 1994-03, Vol.107 (3), p.601-613</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-7f68384a63d6e2f5e4aff4dece9f939720f3f9e6a66b11d98219ac36210dce783</citedby><cites>FETCH-LOGICAL-c407t-7f68384a63d6e2f5e4aff4dece9f939720f3f9e6a66b11d98219ac36210dce783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,3679,27928,27929</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4010445$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8006074$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Forsburg, S.L</creatorcontrib><creatorcontrib>Nurse, P</creatorcontrib><title>Analysis of the Schizosaccharomyces pombe cyclin puc1: evidence for a role in cell cycle exit</title><title>Journal of cell science</title><addtitle>J Cell Sci</addtitle><description>The puc1+ gene, encoding a G1-type cyclin from the fission yeast Schizosaccharomyces pombe, was originally isolated by complementation in the budding yeast Saccharomyces cerevisiae. Here, we report the molecular characterization of this gene and analyse its role in S. pombe. We fail to identify any function of this cyclin at the mitotic G1/S transition in S. pombe, but demonstrate that it does function in exit from the mitotic cycle. Expression of the puc1+ gene is increased during nitrogen starvation, and puc1 affects the timing of sexual development in response to starvation. Overexpression of the puc1 protein blocks sexual development, and rescues pat1ts cells, which would otherwise undergo a lethal meiosis. We conclude that puc1 contributes to negative regulation of the timing of sexual development in fission yeast, and functions at the transition between cycling and non-cycling cells.</description><subject>Alleles</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cell Cycle - genetics</subject><subject>Cell cycle, cell proliferation</subject><subject>Cell physiology</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>Cyclins - analysis</subject><subject>Cyclins - genetics</subject><subject>DNA, Fungal</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - analysis</subject><subject>Fungal Proteins - genetics</subject><subject>genbank/x74451</subject><subject>gene expression</subject><subject>Genes, Fungal</subject><subject>Immunoblotting</subject><subject>meiosis</subject><subject>messenger RNA</subject><subject>mitosis</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Phenotype</subject><subject>proteins</subject><subject>puc1 gene</subject><subject>Reproduction - genetics</subject><subject>Saccharomycetales</subject><subject>Schizosaccharomyces - chemistry</subject><subject>Schizosaccharomyces - cytology</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces - growth & development</subject><subject>Schizosaccharomyces pombe</subject><subject>Schizosaccharomyces pombe Proteins</subject><subject>sexual development</subject><subject>structural genes</subject><issn>0021-9533</issn><issn>1477-9137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0M1LHDEYBvBQWnTVHj2KORRvs775mHz0JqJtQehBPZaQzbxxR2Yma7Jbuv3rjd3Fa0954fnxEB5CThnMGZf88jmUOQM9F3MF7AOZMal1Y5nQH8kMgLPGtkIckqNSngFAc6sPyIEBUKDljPy6mvywLX2hKdL1Eul9WPZ_U_EhLH1O4zZgoas0LpCGbRj6ia42gX2l-LvvcApIY8rU05wGpDUMOAz_IFL8069PyKfoh4Kf9-8xeby9ebj-3tz9_Pbj-uquCRL0utFRGWGkV6JTyGOL0scoOwxooxVWc4giWlReqQVjnTWcWR-E4gy6gNqIY3Kx613l9LLBsnZjX97-4idMm-K0alvTGv5fyJSRrNW6wmYHQ06lZIxulfvR561j4N52d3X3emsnXN29-rN98WYxYveu90PX_Ms-9yX4IWY_hb68MwkMpGwrO9-x6JPzT7mSx3te-4FJIy0I8QpUiJPZ</recordid><startdate>19940301</startdate><enddate>19940301</enddate><creator>Forsburg, S.L</creator><creator>Nurse, P</creator><general>Company of Biologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19940301</creationdate><title>Analysis of the Schizosaccharomyces pombe cyclin puc1: evidence for a role in cell cycle exit</title><author>Forsburg, S.L ; Nurse, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-7f68384a63d6e2f5e4aff4dece9f939720f3f9e6a66b11d98219ac36210dce783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Alleles</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cell Cycle - genetics</topic><topic>Cell cycle, cell proliferation</topic><topic>Cell physiology</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>Cyclins - analysis</topic><topic>Cyclins - genetics</topic><topic>DNA, Fungal</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - analysis</topic><topic>Fungal Proteins - genetics</topic><topic>genbank/x74451</topic><topic>gene expression</topic><topic>Genes, Fungal</topic><topic>Immunoblotting</topic><topic>meiosis</topic><topic>messenger RNA</topic><topic>mitosis</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Phenotype</topic><topic>proteins</topic><topic>puc1 gene</topic><topic>Reproduction - genetics</topic><topic>Saccharomycetales</topic><topic>Schizosaccharomyces - chemistry</topic><topic>Schizosaccharomyces - cytology</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces - growth & development</topic><topic>Schizosaccharomyces pombe</topic><topic>Schizosaccharomyces pombe Proteins</topic><topic>sexual development</topic><topic>structural genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Forsburg, S.L</creatorcontrib><creatorcontrib>Nurse, P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cell science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Forsburg, S.L</au><au>Nurse, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the Schizosaccharomyces pombe cyclin puc1: evidence for a role in cell cycle exit</atitle><jtitle>Journal of cell science</jtitle><addtitle>J Cell Sci</addtitle><date>1994-03-01</date><risdate>1994</risdate><volume>107</volume><issue>3</issue><spage>601</spage><epage>613</epage><pages>601-613</pages><issn>0021-9533</issn><eissn>1477-9137</eissn><coden>JNCSAI</coden><abstract>The puc1+ gene, encoding a G1-type cyclin from the fission yeast Schizosaccharomyces pombe, was originally isolated by complementation in the budding yeast Saccharomyces cerevisiae. Here, we report the molecular characterization of this gene and analyse its role in S. pombe. We fail to identify any function of this cyclin at the mitotic G1/S transition in S. pombe, but demonstrate that it does function in exit from the mitotic cycle. Expression of the puc1+ gene is increased during nitrogen starvation, and puc1 affects the timing of sexual development in response to starvation. Overexpression of the puc1 protein blocks sexual development, and rescues pat1ts cells, which would otherwise undergo a lethal meiosis. We conclude that puc1 contributes to negative regulation of the timing of sexual development in fission yeast, and functions at the transition between cycling and non-cycling cells.</abstract><cop>Cambridge</cop><pub>Company of Biologists</pub><pmid>8006074</pmid><doi>10.1242/jcs.107.3.601</doi><tpages>13</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Company of Biologists |
| subjects | Alleles Amino Acid Sequence amino acid sequences Base Sequence Biological and medical sciences Blotting, Northern Cell Cycle - genetics Cell cycle, cell proliferation Cell physiology Cloning, Molecular complementary DNA Cyclins - analysis Cyclins - genetics DNA, Fungal Fundamental and applied biological sciences. Psychology Fungal Proteins - analysis Fungal Proteins - genetics genbank/x74451 gene expression Genes, Fungal Immunoblotting meiosis messenger RNA mitosis Molecular and cellular biology Molecular Sequence Data nucleotide sequences Phenotype proteins puc1 gene Reproduction - genetics Saccharomycetales Schizosaccharomyces - chemistry Schizosaccharomyces - cytology Schizosaccharomyces - genetics Schizosaccharomyces - growth & development Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins sexual development structural genes |
| title | Analysis of the Schizosaccharomyces pombe cyclin puc1: evidence for a role in cell cycle exit |
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