Subtle differences in human pregnancy-specific glycoprotein gene promoters allow for differential expression
Eleven pregnancy-specific glycoprotein (PSG) genes reside on human chromosome 19. The sequence of these genes is extremely similar and that similarity extends to their putative control regions. However, the expression pattern of each PSG gene differs in the placenta, the primary site of PSG synthesi...
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| Veröffentlicht in: | The Journal of biological chemistry 1994-06, Vol.269 (25), p.17152-17159 |
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| creator | CHAMBERLIN, M. E KE-JIAN LEI YANG CHOU, J |
| description | Eleven pregnancy-specific glycoprotein (PSG) genes reside on human chromosome 19. The sequence of these genes is extremely
similar and that similarity extends to their putative control regions. However, the expression pattern of each PSG gene differs
in the placenta, the primary site of PSG synthesis. To understand the molecular mechanisms underlying differential PSG expression,
we characterized promoter elements of six PSG genes. We have shown previously that nucleotides -172 to -34 with respect to
the translation start site constitute a minimal promoter in the PSG12 gene (class 1). We now show that PSG1-I and PSG3 are
also members of class 1 genes. In contrast, only nucleotides -172 to -80 are necessary for promoter activity in PSG5, PSG6,
and PSG11 genes (class 2). Class 2 genes contain a perfect Sp1 recognition sequence (CCCCGCCC) at nucleotides -148 to -141
which is necessary for promoter activity. Placental cell extracts formed three protein-DNA complexes with nucleotides -172
to -80 of all six PSG genes. One of the components of these complexes is an Sp1-like molecule. We have previously reported
activator sequences within nucleotides -83 to -34 in PSG12. We now show that a 50-kDa protein binds to this region of PSG12,
and the resultant complex can be supershifted by a monoclonal antibody to PEA3. |
| doi_str_mv | 10.1016/S0021-9258(17)32534-6 |
| format | Article |
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similar and that similarity extends to their putative control regions. However, the expression pattern of each PSG gene differs
in the placenta, the primary site of PSG synthesis. To understand the molecular mechanisms underlying differential PSG expression,
we characterized promoter elements of six PSG genes. We have shown previously that nucleotides -172 to -34 with respect to
the translation start site constitute a minimal promoter in the PSG12 gene (class 1). We now show that PSG1-I and PSG3 are
also members of class 1 genes. In contrast, only nucleotides -172 to -80 are necessary for promoter activity in PSG5, PSG6,
and PSG11 genes (class 2). Class 2 genes contain a perfect Sp1 recognition sequence (CCCCGCCC) at nucleotides -148 to -141
which is necessary for promoter activity. Placental cell extracts formed three protein-DNA complexes with nucleotides -172
to -80 of all six PSG genes. One of the components of these complexes is an Sp1-like molecule. We have previously reported
activator sequences within nucleotides -83 to -34 in PSG12. We now show that a 50-kDa protein binds to this region of PSG12,
and the resultant complex can be supershifted by a monoclonal antibody to PEA3.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(17)32534-6</identifier><identifier>PMID: 8006022</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Base Sequence ; Biological and medical sciences ; Chromosomes, Human, Pair 19 ; Cross-Linking Reagents ; DNA-Binding Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Glycoproteins - genetics ; Humans ; Macromolecular Substances ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Oligonucleotide Probes - chemistry ; Pregnancy Proteins - genetics ; Pregnancy-Specific beta 1-Glycoproteins ; Promoter Regions, Genetic ; RNA, Messenger - genetics ; Sequence Alignment ; Sequence Homology, Nucleic Acid ; Sp1 Transcription Factor - metabolism ; Transcription Factors - metabolism ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>The Journal of biological chemistry, 1994-06, Vol.269 (25), p.17152-17159</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-a36b1b43e943bc303dae17de1e7f407455136402a56881921ff5be7f853176523</citedby><cites>FETCH-LOGICAL-c440t-a36b1b43e943bc303dae17de1e7f407455136402a56881921ff5be7f853176523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4207526$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8006022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHAMBERLIN, M. E</creatorcontrib><creatorcontrib>KE-JIAN LEI</creatorcontrib><creatorcontrib>YANG CHOU, J</creatorcontrib><title>Subtle differences in human pregnancy-specific glycoprotein gene promoters allow for differential expression</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Eleven pregnancy-specific glycoprotein (PSG) genes reside on human chromosome 19. The sequence of these genes is extremely
similar and that similarity extends to their putative control regions. However, the expression pattern of each PSG gene differs
in the placenta, the primary site of PSG synthesis. To understand the molecular mechanisms underlying differential PSG expression,
we characterized promoter elements of six PSG genes. We have shown previously that nucleotides -172 to -34 with respect to
the translation start site constitute a minimal promoter in the PSG12 gene (class 1). We now show that PSG1-I and PSG3 are
also members of class 1 genes. In contrast, only nucleotides -172 to -80 are necessary for promoter activity in PSG5, PSG6,
and PSG11 genes (class 2). Class 2 genes contain a perfect Sp1 recognition sequence (CCCCGCCC) at nucleotides -148 to -141
which is necessary for promoter activity. Placental cell extracts formed three protein-DNA complexes with nucleotides -172
to -80 of all six PSG genes. One of the components of these complexes is an Sp1-like molecule. We have previously reported
activator sequences within nucleotides -83 to -34 in PSG12. We now show that a 50-kDa protein binds to this region of PSG12,
and the resultant complex can be supershifted by a monoclonal antibody to PEA3.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosomes, Human, Pair 19</subject><subject>Cross-Linking Reagents</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Glycoproteins - genetics</subject><subject>Humans</subject><subject>Macromolecular Substances</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotide Probes - chemistry</subject><subject>Pregnancy Proteins - genetics</subject><subject>Pregnancy-Specific beta 1-Glycoproteins</subject><subject>Promoter Regions, Genetic</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Sp1 Transcription Factor - metabolism</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription. Transcription factor. Splicing. 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E</creator><creator>KE-JIAN LEI</creator><creator>YANG CHOU, J</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19940624</creationdate><title>Subtle differences in human pregnancy-specific glycoprotein gene promoters allow for differential expression</title><author>CHAMBERLIN, M. E ; KE-JIAN LEI ; YANG CHOU, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-a36b1b43e943bc303dae17de1e7f407455136402a56881921ff5be7f853176523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosomes, Human, Pair 19</topic><topic>Cross-Linking Reagents</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Glycoproteins - genetics</topic><topic>Humans</topic><topic>Macromolecular Substances</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotide Probes - chemistry</topic><topic>Pregnancy Proteins - genetics</topic><topic>Pregnancy-Specific beta 1-Glycoproteins</topic><topic>Promoter Regions, Genetic</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Sp1 Transcription Factor - metabolism</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHAMBERLIN, M. E</creatorcontrib><creatorcontrib>KE-JIAN LEI</creatorcontrib><creatorcontrib>YANG CHOU, J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHAMBERLIN, M. E</au><au>KE-JIAN LEI</au><au>YANG CHOU, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Subtle differences in human pregnancy-specific glycoprotein gene promoters allow for differential expression</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1994-06-24</date><risdate>1994</risdate><volume>269</volume><issue>25</issue><spage>17152</spage><epage>17159</epage><pages>17152-17159</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Eleven pregnancy-specific glycoprotein (PSG) genes reside on human chromosome 19. The sequence of these genes is extremely
similar and that similarity extends to their putative control regions. However, the expression pattern of each PSG gene differs
in the placenta, the primary site of PSG synthesis. To understand the molecular mechanisms underlying differential PSG expression,
we characterized promoter elements of six PSG genes. We have shown previously that nucleotides -172 to -34 with respect to
the translation start site constitute a minimal promoter in the PSG12 gene (class 1). We now show that PSG1-I and PSG3 are
also members of class 1 genes. In contrast, only nucleotides -172 to -80 are necessary for promoter activity in PSG5, PSG6,
and PSG11 genes (class 2). Class 2 genes contain a perfect Sp1 recognition sequence (CCCCGCCC) at nucleotides -148 to -141
which is necessary for promoter activity. Placental cell extracts formed three protein-DNA complexes with nucleotides -172
to -80 of all six PSG genes. One of the components of these complexes is an Sp1-like molecule. We have previously reported
activator sequences within nucleotides -83 to -34 in PSG12. We now show that a 50-kDa protein binds to this region of PSG12,
and the resultant complex can be supershifted by a monoclonal antibody to PEA3.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8006022</pmid><doi>10.1016/S0021-9258(17)32534-6</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Base Sequence Biological and medical sciences Chromosomes, Human, Pair 19 Cross-Linking Reagents DNA-Binding Proteins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation Glycoproteins - genetics Humans Macromolecular Substances Molecular and cellular biology Molecular genetics Molecular Sequence Data Oligonucleotide Probes - chemistry Pregnancy Proteins - genetics Pregnancy-Specific beta 1-Glycoproteins Promoter Regions, Genetic RNA, Messenger - genetics Sequence Alignment Sequence Homology, Nucleic Acid Sp1 Transcription Factor - metabolism Transcription Factors - metabolism Transcription. Transcription factor. Splicing. Rna processing |
| title | Subtle differences in human pregnancy-specific glycoprotein gene promoters allow for differential expression |
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