cDNA-Derived Hypovirus RNA in Transformed Chestnut Blight Fungus Is Spliced and Trimmed of Vector Nucleotides
Unencapsidated double-stranded viral RNAs belonging to the genus Hypovirus attenuate virulence of the chestnut blight fungus, Cryphonectria parasitica . A full-length cDNA clone of hypovirus CHV1-713 double-stranded RNA was recently shown to be infectious when introduced into the C. parasitica genom...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1994-07, Vol.202 (1), p.441-448 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Chen, Baoshan Craven, Mark G. Choi, Gil H. Nuss, Donald L. |
| description | Unencapsidated double-stranded viral RNAs belonging to the genus
Hypovirus attenuate virulence of the chestnut blight fungus,
Cryphonectria parasitica . A full-length cDNA clone of hypovirus CHV1-713 double-stranded RNA was recently shown to be infectious when introduced into the
C. parasitica genome by DNA-mediated transformation. In this study, we show that the viral RNA derived from the chromosomally integrated cDNA copy is trimmed of extraneous vector nucleotide sequences. The cDNA-derived viral RNA was also found to contain a 73-bp deletion located within the 5′-noncoding leader sequence as a result of a pre-mRNA splicing event. Implications of these results are discussed in terms of hypovirus RNA replication and anticipated field studies involving engineered hypovirulent
C. parasitica strains. |
| doi_str_mv | 10.1006/viro.1994.1360 |
| format | Article |
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Hypovirus attenuate virulence of the chestnut blight fungus,
Cryphonectria parasitica . A full-length cDNA clone of hypovirus CHV1-713 double-stranded RNA was recently shown to be infectious when introduced into the
C. parasitica genome by DNA-mediated transformation. In this study, we show that the viral RNA derived from the chromosomally integrated cDNA copy is trimmed of extraneous vector nucleotide sequences. The cDNA-derived viral RNA was also found to contain a 73-bp deletion located within the 5′-noncoding leader sequence as a result of a pre-mRNA splicing event. Implications of these results are discussed in terms of hypovirus RNA replication and anticipated field studies involving engineered hypovirulent
C. parasitica strains.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1006/viro.1994.1360</identifier><identifier>PMID: 8009855</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Agronomy. Soil science and plant productions ; arn ; Base Sequence ; Biological and medical sciences ; Cryphonectria parasitica ; DNA, Complementary - genetics ; DNA, Viral - genetics ; Fundamental and applied biological sciences. Psychology ; genetic transformation ; Genetic Vectors ; Genetics ; hypovirus ; Microbiology ; Molecular Sequence Data ; nucleotide sequence ; pathogenicity ; plant viruses ; poder patogeno ; pouvoir pathogene ; rna ; RNA Splicing ; RNA Viruses - genetics ; RNA, Viral - genetics ; RNA, Viral - metabolism ; secuencia nucleica ; sequence nucleique ; transformacion genetica ; transformation genetique ; Transformation, Genetic ; Virology ; Virulence - genetics ; virus de las plantas ; virus des vegetaux ; Xylariales - genetics ; Xylariales - pathogenicity</subject><ispartof>Virology (New York, N.Y.), 1994-07, Vol.202 (1), p.441-448</ispartof><rights>1994 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-a9ec88614df4288bcd0adeb35017cf2d108cf92bc06183b3060be9172bd53cd43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/viro.1994.1360$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4199759$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8009855$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Baoshan</creatorcontrib><creatorcontrib>Craven, Mark G.</creatorcontrib><creatorcontrib>Choi, Gil H.</creatorcontrib><creatorcontrib>Nuss, Donald L.</creatorcontrib><creatorcontrib>FAO, Rome (Italy). Div. de la Production Vegetale et de la Protection des Plantes</creatorcontrib><title>cDNA-Derived Hypovirus RNA in Transformed Chestnut Blight Fungus Is Spliced and Trimmed of Vector Nucleotides</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Unencapsidated double-stranded viral RNAs belonging to the genus
Hypovirus attenuate virulence of the chestnut blight fungus,
Cryphonectria parasitica . A full-length cDNA clone of hypovirus CHV1-713 double-stranded RNA was recently shown to be infectious when introduced into the
C. parasitica genome by DNA-mediated transformation. In this study, we show that the viral RNA derived from the chromosomally integrated cDNA copy is trimmed of extraneous vector nucleotide sequences. The cDNA-derived viral RNA was also found to contain a 73-bp deletion located within the 5′-noncoding leader sequence as a result of a pre-mRNA splicing event. Implications of these results are discussed in terms of hypovirus RNA replication and anticipated field studies involving engineered hypovirulent
C. parasitica strains.</description><subject>Agronomy. Soil science and plant productions</subject><subject>arn</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cryphonectria parasitica</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Viral - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetic transformation</subject><subject>Genetic Vectors</subject><subject>Genetics</subject><subject>hypovirus</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequence</subject><subject>pathogenicity</subject><subject>plant viruses</subject><subject>poder patogeno</subject><subject>pouvoir pathogene</subject><subject>rna</subject><subject>RNA Splicing</subject><subject>RNA Viruses - genetics</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - metabolism</subject><subject>secuencia nucleica</subject><subject>sequence nucleique</subject><subject>transformacion genetica</subject><subject>transformation genetique</subject><subject>Transformation, Genetic</subject><subject>Virology</subject><subject>Virulence - genetics</subject><subject>virus de las plantas</subject><subject>virus des vegetaux</subject><subject>Xylariales - genetics</subject><subject>Xylariales - pathogenicity</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EKkvhyg2UA-KWZZzEX8dlSz-kapFoy9VybGdrlI0XO1mp_z0T7aq3ipNlvd88zbxHyEcKSwrAvx1CikuqVLOkNYdXZEFB8RLqhr4mC4CmKrmsqrfkXc5_AP9CwBk5kwBKMrYgO3uxWZUXPoWDd8X10z6i4ZSLX5tVEYbiPpkhdzHtUFw_-jwO01h878P2cSwup2GL5E0u7vZ9sEiYweFE2M107Irf3o4xFZvJ9j6Owfn8nrzpTJ_9h9N7Th4uf9yvr8vbn1c369VtaZuqGkujvJWS08Z1TSVlax0Y59uaARW2qxwFaTtVtRY4lXVbA4fWKyqq1rHauqY-J1-PvvsU_064tt6FbH3fm8HHKWvBGaNMiP-ClCtKJQUEl0fQpphz8p3e46EmPWkKei5Cz0XouQg9F4EDn07OU4t5POOn5FH_ctJNtqbvMGgb8jPWoJNgCrHPR6wzUZttQuThDjUGICQuh4A8Ah7zPASfdLbBD1hHSJi_djG8tOI_Lc2tLw</recordid><startdate>19940701</startdate><enddate>19940701</enddate><creator>Chen, Baoshan</creator><creator>Craven, Mark G.</creator><creator>Choi, Gil H.</creator><creator>Nuss, Donald L.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19940701</creationdate><title>cDNA-Derived Hypovirus RNA in Transformed Chestnut Blight Fungus Is Spliced and Trimmed of Vector Nucleotides</title><author>Chen, Baoshan ; Craven, Mark G. ; Choi, Gil H. ; Nuss, Donald L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-a9ec88614df4288bcd0adeb35017cf2d108cf92bc06183b3060be9172bd53cd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>arn</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cryphonectria parasitica</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Viral - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetic transformation</topic><topic>Genetic Vectors</topic><topic>Genetics</topic><topic>hypovirus</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequence</topic><topic>pathogenicity</topic><topic>plant viruses</topic><topic>poder patogeno</topic><topic>pouvoir pathogene</topic><topic>rna</topic><topic>RNA Splicing</topic><topic>RNA Viruses - genetics</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - metabolism</topic><topic>secuencia nucleica</topic><topic>sequence nucleique</topic><topic>transformacion genetica</topic><topic>transformation genetique</topic><topic>Transformation, Genetic</topic><topic>Virology</topic><topic>Virulence - genetics</topic><topic>virus de las plantas</topic><topic>virus des vegetaux</topic><topic>Xylariales - genetics</topic><topic>Xylariales - pathogenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Baoshan</creatorcontrib><creatorcontrib>Craven, Mark G.</creatorcontrib><creatorcontrib>Choi, Gil H.</creatorcontrib><creatorcontrib>Nuss, Donald L.</creatorcontrib><creatorcontrib>FAO, Rome (Italy). 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Div. de la Production Vegetale et de la Protection des Plantes</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cDNA-Derived Hypovirus RNA in Transformed Chestnut Blight Fungus Is Spliced and Trimmed of Vector Nucleotides</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1994-07-01</date><risdate>1994</risdate><volume>202</volume><issue>1</issue><spage>441</spage><epage>448</epage><pages>441-448</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>Unencapsidated double-stranded viral RNAs belonging to the genus
Hypovirus attenuate virulence of the chestnut blight fungus,
Cryphonectria parasitica . A full-length cDNA clone of hypovirus CHV1-713 double-stranded RNA was recently shown to be infectious when introduced into the
C. parasitica genome by DNA-mediated transformation. In this study, we show that the viral RNA derived from the chromosomally integrated cDNA copy is trimmed of extraneous vector nucleotide sequences. The cDNA-derived viral RNA was also found to contain a 73-bp deletion located within the 5′-noncoding leader sequence as a result of a pre-mRNA splicing event. Implications of these results are discussed in terms of hypovirus RNA replication and anticipated field studies involving engineered hypovirulent
C. parasitica strains.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8009855</pmid><doi>10.1006/viro.1994.1360</doi><tpages>8</tpages></addata></record> |
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| ispartof | Virology (New York, N.Y.), 1994-07, Vol.202 (1), p.441-448 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals |
| subjects | Agronomy. Soil science and plant productions arn Base Sequence Biological and medical sciences Cryphonectria parasitica DNA, Complementary - genetics DNA, Viral - genetics Fundamental and applied biological sciences. Psychology genetic transformation Genetic Vectors Genetics hypovirus Microbiology Molecular Sequence Data nucleotide sequence pathogenicity plant viruses poder patogeno pouvoir pathogene rna RNA Splicing RNA Viruses - genetics RNA, Viral - genetics RNA, Viral - metabolism secuencia nucleica sequence nucleique transformacion genetica transformation genetique Transformation, Genetic Virology Virulence - genetics virus de las plantas virus des vegetaux Xylariales - genetics Xylariales - pathogenicity |
| title | cDNA-Derived Hypovirus RNA in Transformed Chestnut Blight Fungus Is Spliced and Trimmed of Vector Nucleotides |
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