Monoclonal IgM in a patient with paraproteinemic polyneuropathy binds to gangliosides containing disialosyl groups
Monoclonal IgM K from a patient with polyneuropathy associated with paraproteinemia was found to bind to several polysialogangliosides. Binding of IgM to gangliosides was shown by enzyme‐linked immunosorbent assay (ELISA) and by overlaying thin‐layer chromatograms of human brain and peripheral nerve...
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| Veröffentlicht in: | Annals of neurology 1985-12, Vol.18 (6), p.655-659 |
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| container_end_page | 659 |
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| container_issue | 6 |
| container_start_page | 655 |
| container_title | Annals of neurology |
| container_volume | 18 |
| creator | Ilyas, Amjad A. Quarles, Richard H. Dalakas, Marinos C. Fishman, Peter H. Brady, Roscoe O. |
| description | Monoclonal IgM K from a patient with polyneuropathy associated with paraproteinemia was found to bind to several polysialogangliosides. Binding of IgM to gangliosides was shown by enzyme‐linked immunosorbent assay (ELISA) and by overlaying thin‐layer chromatograms of human brain and peripheral nerve gangliosides with the patient's serum followed by radioiodinated goat antihuman IgM. The latter technique showed that the IgM paraprotein reacted with a number of gangliosides. In an ELISA the IgM paraprotein reacted strongly with GD2, GD3, GD1b, and GT1b, but not with GM1, GM3, and GD1a. Thus, the epitope for the patient's IgM paraprotein appears to involve the disialosyl configurations. |
| doi_str_mv | 10.1002/ana.410180605 |
| format | Article |
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Binding of IgM to gangliosides was shown by enzyme‐linked immunosorbent assay (ELISA) and by overlaying thin‐layer chromatograms of human brain and peripheral nerve gangliosides with the patient's serum followed by radioiodinated goat antihuman IgM. The latter technique showed that the IgM paraprotein reacted with a number of gangliosides. In an ELISA the IgM paraprotein reacted strongly with GD2, GD3, GD1b, and GT1b, but not with GM1, GM3, and GD1a. 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Binding of IgM to gangliosides was shown by enzyme‐linked immunosorbent assay (ELISA) and by overlaying thin‐layer chromatograms of human brain and peripheral nerve gangliosides with the patient's serum followed by radioiodinated goat antihuman IgM. The latter technique showed that the IgM paraprotein reacted with a number of gangliosides. In an ELISA the IgM paraprotein reacted strongly with GD2, GD3, GD1b, and GT1b, but not with GM1, GM3, and GD1a. Thus, the epitope for the patient's IgM paraprotein appears to involve the disialosyl configurations.</description><subject>Biological and medical sciences</subject><subject>Brain Chemistry</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Epitopes - analysis</subject><subject>Gangliosides - isolation & purification</subject><subject>Humans</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunoglobulin M</subject><subject>Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Paraproteinemias - blood</subject><subject>Paraproteins</subject><subject>Peripheral Nerves - analysis</subject><subject>Peripheral Nervous System Diseases - blood</subject><issn>0364-5134</issn><issn>1531-8249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1v1DAQxS0EKkvhyBHJB8QtxY4_khxXFd1WapcDhXKzJsns1uC1g52o5L_HaKMVJ06j0fvNzJtHyFvOLjhj5UfwcCE54zXTTD0jK64EL-pSNs_JigktC8WFfElepfSDMdZozs7IWSl5paRYkXgXfOhc8ODozf6OWk-BDjBa9CN9suNjbiIMMYxoPR5sR4fgZo9TDJl6nGlrfZ_oGOge_N7ZkGyPiXbBj2C99Xva22TBhTQ7uo9hGtJr8mIHLuGbpZ6Tr1ef7i-vi9vPm5vL9W3RSSZUIXsOvd4xaPpWcc6RQ8uAqRpbRBCdzjK2jdIVQt1IrKGFMn9V9m3XKyzFOflw3Jvd_5owjeZgU4fOgccwJVNpJWtesQwWR7CLIaWIOzNEe4A4G87M34xNzticMs78u2Xx1B6wP9FLqFl_v-iQOnC7CL6z6YTVmkuhm4xVR-zJOpz_f9Ost-t_DSyGbRrx92kS4k-jK1Ep87DdmG9fttff7x825kr8AVPCp0k</recordid><startdate>198512</startdate><enddate>198512</enddate><creator>Ilyas, Amjad A.</creator><creator>Quarles, Richard H.</creator><creator>Dalakas, Marinos C.</creator><creator>Fishman, Peter H.</creator><creator>Brady, Roscoe O.</creator><general>Little, Brown and Company</general><general>Willey-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198512</creationdate><title>Monoclonal IgM in a patient with paraproteinemic polyneuropathy binds to gangliosides containing disialosyl groups</title><author>Ilyas, Amjad A. ; Quarles, Richard H. ; Dalakas, Marinos C. ; Fishman, Peter H. ; Brady, Roscoe O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4035-4d1ad6f0a9db5111e1ab0a058ebeea3c61adeb9567ea894e8aba21752dbcd5e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Biological and medical sciences</topic><topic>Brain Chemistry</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Epitopes - analysis</topic><topic>Gangliosides - isolation & purification</topic><topic>Humans</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunoglobulin M</topic><topic>Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Paraproteinemias - blood</topic><topic>Paraproteins</topic><topic>Peripheral Nerves - analysis</topic><topic>Peripheral Nervous System Diseases - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ilyas, Amjad A.</creatorcontrib><creatorcontrib>Quarles, Richard H.</creatorcontrib><creatorcontrib>Dalakas, Marinos C.</creatorcontrib><creatorcontrib>Fishman, Peter H.</creatorcontrib><creatorcontrib>Brady, Roscoe O.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ilyas, Amjad A.</au><au>Quarles, Richard H.</au><au>Dalakas, Marinos C.</au><au>Fishman, Peter H.</au><au>Brady, Roscoe O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal IgM in a patient with paraproteinemic polyneuropathy binds to gangliosides containing disialosyl groups</atitle><jtitle>Annals of neurology</jtitle><addtitle>Ann Neurol</addtitle><date>1985-12</date><risdate>1985</risdate><volume>18</volume><issue>6</issue><spage>655</spage><epage>659</epage><pages>655-659</pages><issn>0364-5134</issn><eissn>1531-8249</eissn><coden>ANNED3</coden><abstract>Monoclonal IgM K from a patient with polyneuropathy associated with paraproteinemia was found to bind to several polysialogangliosides. Binding of IgM to gangliosides was shown by enzyme‐linked immunosorbent assay (ELISA) and by overlaying thin‐layer chromatograms of human brain and peripheral nerve gangliosides with the patient's serum followed by radioiodinated goat antihuman IgM. The latter technique showed that the IgM paraprotein reacted with a number of gangliosides. In an ELISA the IgM paraprotein reacted strongly with GD2, GD3, GD1b, and GT1b, but not with GM1, GM3, and GD1a. Thus, the epitope for the patient's IgM paraprotein appears to involve the disialosyl configurations.</abstract><cop>Boston</cop><pub>Little, Brown and Company</pub><pmid>2417543</pmid><doi>10.1002/ana.410180605</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0364-5134 |
| ispartof | Annals of neurology, 1985-12, Vol.18 (6), p.655-659 |
| issn | 0364-5134 1531-8249 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Biological and medical sciences Brain Chemistry Chemical Phenomena Chemistry Epitopes - analysis Gangliosides - isolation & purification Humans Immunodeficiencies. Immunoglobulinopathies Immunoglobulin M Immunoglobulinopathies Immunopathology Male Medical sciences Middle Aged Paraproteinemias - blood Paraproteins Peripheral Nerves - analysis Peripheral Nervous System Diseases - blood |
| title | Monoclonal IgM in a patient with paraproteinemic polyneuropathy binds to gangliosides containing disialosyl groups |
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