Purification and characterization of the extracellular β-glucosidase produced by Candida wickerhamii

Candida wickerhamii NRRL Y-2563 produced a cell-bound β-glucosidase when grown in complex media containing 50 g of cellobiose per liter. The majority of the enzyme was located on the cell surface and was released into the supernatant upon treatment of intact cells with Zymolyase 60,000. Only about 1...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1985-12, Vol.243 (2), p.515-522
1. Verfasser:	Freer, Shelby N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Applied sciences beta-Glucosidase - isolation & purification Candida - enzymology Carbohydrates - analysis Electrophoresis, Polyacrylamide Gel Exact sciences and technology Glucosidases - isolation & purification Hot Temperature Hydrogen-Ion Concentration Isoelectric Point Kinetics Molecular Weight Other techniques and industries
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