Cytochemical features common to nucleoli and cytoplasmic nucleoloids of Olea europaea meiocytes : detection of rRNA by in situ hybridization
We used light and electron microscopic techniques to study the composition of cytoplasmic nucleoloids during meiotic division in Olea europaea. Nucleoloids were found in two clearly distinguishable morphological varieties: one similar in morphology to the nucleolus, and composed mainly of dense fibr...
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| Veröffentlicht in: | Journal of cell science 1994-02, Vol.107 (2), p.621-629 |
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| container_title | Journal of cell science |
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| creator | ALCHE, J. D FERNANDEZ, M. C RODRIGUEZ-GARCIA, M. I |
| description | We used light and electron microscopic techniques to study the composition of cytoplasmic nucleoloids during meiotic division in Olea europaea. Nucleoloids were found in two clearly distinguishable morphological varieties: one similar in morphology to the nucleolus, and composed mainly of dense fibrillar component, and another surrounded by many ribosome-like particles. Cytochemical and immunocytochemical techniques showed similar reactivities in nucleoloids and the nucleolus: both are ribonucleoproteic in nature, and possess argyrophillic, argentaffinic and highly phosphorylated proteins. Immunohistochemical techniques failed to detect DNA in either structure. In situ hybridization to a 18 S rRNA probe demonstrated the presence of ribosomal transcripts in both the nucleolus and nucleoloids. These similarities in morphology and composition may reflect similar functionalities. |
| doi_str_mv | 10.1242/jcs.107.2.621 |
| format | Article |
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D</creatorcontrib><creatorcontrib>FERNANDEZ, M. C</creatorcontrib><creatorcontrib>RODRIGUEZ-GARCIA, M. I</creatorcontrib><title>Cytochemical features common to nucleoli and cytoplasmic nucleoloids of Olea europaea meiocytes : detection of rRNA by in situ hybridization</title><title>Journal of cell science</title><addtitle>J Cell Sci</addtitle><description>We used light and electron microscopic techniques to study the composition of cytoplasmic nucleoloids during meiotic division in Olea europaea. Nucleoloids were found in two clearly distinguishable morphological varieties: one similar in morphology to the nucleolus, and composed mainly of dense fibrillar component, and another surrounded by many ribosome-like particles. Cytochemical and immunocytochemical techniques showed similar reactivities in nucleoloids and the nucleolus: both are ribonucleoproteic in nature, and possess argyrophillic, argentaffinic and highly phosphorylated proteins. Immunohistochemical techniques failed to detect DNA in either structure. In situ hybridization to a 18 S rRNA probe demonstrated the presence of ribosomal transcripts in both the nucleolus and nucleoloids. These similarities in morphology and composition may reflect similar functionalities.</description><subject>Biological and medical sciences</subject><subject>Cell Nucleolus - metabolism</subject><subject>Cell Nucleolus - ultrastructure</subject><subject>Cell structures and functions</subject><subject>Cytoplasm - metabolism</subject><subject>Cytoplasm - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Inclusion Bodies - metabolism</subject><subject>Inclusion Bodies - ultrastructure</subject><subject>Meiosis</subject><subject>Microscopy, Electron</subject><subject>Miscellaneous</subject><subject>Molecular and cellular biology</subject><subject>Olea europaea</subject><subject>Plant Proteins - metabolism</subject><subject>RNA, Ribosomal, 18S - genetics</subject><subject>RNA, Ribosomal, 18S - metabolism</subject><subject>Trees - genetics</subject><subject>Trees - metabolism</subject><subject>Trees - ultrastructure</subject><issn>0021-9533</issn><issn>1477-9137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UFL5TAUBeAgir5xXM5yIAtx1ze5SdPbupOHzgiiMMysS5rcYqRtnkm7eP6G-dFGfLqdVS6cj0PgMPYNxBpkKX882bQGgWu5riQcsBWUiEUDCg_ZSggJRaOVOmFfUnoSQqBs8Jgd11KgqMsV-7fZzcE-0uitGXhPZl4iJW7DOIaJz4FPix0oDJ6byXGb8XYwKeuPIHiXeOj5w0CG0xLD1uRjJB8yzk2X3NFMdva5LrP4-_6KdzvuJ578vPDHXRe98y_mDXxlR70ZEp3t31P29-b6z-ZXcffw83ZzdVdYpRAKK0pBGklVyvVCNz24TpVliUYjOqM7aJBkV2ujBVithFOytlVt0UKldadO2cV77zaG54XS3I4-WRoGM1FYUouVLiWA-i-EqhKoKplh8Q5tDClF6ttt9KOJuxZE-zZTm2fKN7ayzTNl_31fvHQjuU-93yXn5_vcpDxMH81kffpkqqnz70C9Auu5nCQ</recordid><startdate>199402</startdate><enddate>199402</enddate><creator>ALCHE, J. 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I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3371-c040e57e363df059f1db34447a577da5b197e2b85a501c530d328c68c7c1655b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Biological and medical sciences</topic><topic>Cell Nucleolus - metabolism</topic><topic>Cell Nucleolus - ultrastructure</topic><topic>Cell structures and functions</topic><topic>Cytoplasm - metabolism</topic><topic>Cytoplasm - ultrastructure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Inclusion Bodies - metabolism</topic><topic>Inclusion Bodies - ultrastructure</topic><topic>Meiosis</topic><topic>Microscopy, Electron</topic><topic>Miscellaneous</topic><topic>Molecular and cellular biology</topic><topic>Olea europaea</topic><topic>Plant Proteins - metabolism</topic><topic>RNA, Ribosomal, 18S - genetics</topic><topic>RNA, Ribosomal, 18S - metabolism</topic><topic>Trees - genetics</topic><topic>Trees - metabolism</topic><topic>Trees - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ALCHE, J. D</creatorcontrib><creatorcontrib>FERNANDEZ, M. C</creatorcontrib><creatorcontrib>RODRIGUEZ-GARCIA, M. 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I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytochemical features common to nucleoli and cytoplasmic nucleoloids of Olea europaea meiocytes : detection of rRNA by in situ hybridization</atitle><jtitle>Journal of cell science</jtitle><addtitle>J Cell Sci</addtitle><date>1994-02</date><risdate>1994</risdate><volume>107</volume><issue>2</issue><spage>621</spage><epage>629</epage><pages>621-629</pages><issn>0021-9533</issn><eissn>1477-9137</eissn><coden>JNCSAI</coden><abstract>We used light and electron microscopic techniques to study the composition of cytoplasmic nucleoloids during meiotic division in Olea europaea. Nucleoloids were found in two clearly distinguishable morphological varieties: one similar in morphology to the nucleolus, and composed mainly of dense fibrillar component, and another surrounded by many ribosome-like particles. Cytochemical and immunocytochemical techniques showed similar reactivities in nucleoloids and the nucleolus: both are ribonucleoproteic in nature, and possess argyrophillic, argentaffinic and highly phosphorylated proteins. Immunohistochemical techniques failed to detect DNA in either structure. In situ hybridization to a 18 S rRNA probe demonstrated the presence of ribosomal transcripts in both the nucleolus and nucleoloids. These similarities in morphology and composition may reflect similar functionalities.</abstract><cop>Cambridge</cop><pub>Company of Biologists</pub><pmid>8207084</pmid><doi>10.1242/jcs.107.2.621</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of cell science, 1994-02, Vol.107 (2), p.621-629 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Company of Biologists |
| subjects | Biological and medical sciences Cell Nucleolus - metabolism Cell Nucleolus - ultrastructure Cell structures and functions Cytoplasm - metabolism Cytoplasm - ultrastructure Fundamental and applied biological sciences. Psychology Immunohistochemistry In Situ Hybridization Inclusion Bodies - metabolism Inclusion Bodies - ultrastructure Meiosis Microscopy, Electron Miscellaneous Molecular and cellular biology Olea europaea Plant Proteins - metabolism RNA, Ribosomal, 18S - genetics RNA, Ribosomal, 18S - metabolism Trees - genetics Trees - metabolism Trees - ultrastructure |
| title | Cytochemical features common to nucleoli and cytoplasmic nucleoloids of Olea europaea meiocytes : detection of rRNA by in situ hybridization |
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